Related ArticlesTranscriptional enhancer related DNA sequences: anomalous 1H NMR NOE crosspeaks.
Nucleic Acids Res. 1992 Feb 11;20(3):525-32
Authors: Donlan ME, Lu P
A dynamic heterogeneity which correlates with the function of the operator DNA in the lactose operon of E. coli. was previously observed (1) as a local minimum in the thymine imino proton T1 centered at a GTG/C-CAC sequence. Since this triplet occurs frequently in DNA regulatory regions, it was proposed that these sequences may be part of a structural element for specific protein interaction. We examine here three additional biologically significant 17 base pair duplexes containing GTG/CAC triplets: (1) a sequence from the mouse heavy chain immunoglobulin enhancer, (2) a sequence from the critical core of the Simian Virus 40 (SV40) enhancer, and (3) a sequence from pBR322 plasmid used as control for experiments with the SV40 DNA sequences. The 1H NMR resonance assignment for nearly all the nonexchangeable protons for both eukaryotic enhancer duplexes with the exception of the H5'/H5" protons was accomplished to use for structural analysis of these duplexes. The data presented show several NOE's associated with the GTG/CAC triplets which suggest structural variation from uniform B-DNA. In addition, anomalous broad crosspeaks for the fixed thymine methyl to its own H6 proton in combination with the imino proton kinetics associated with these triplets reinforces the original observation of a sequence dependent dynamic variation.
[NMR Sparky Yahoo group] Re: distance constraints from NOESY crosspeaks
Re: distance constraints from NOESY crosspeaks
Did you do the build up with different mixing time?Jay From: jennig148 To: nmr_sparky@yahoogroups.com Sent: Friday, February 10,
More...
nmrlearner
News from other NMR forums
0
02-10-2012 09:28 PM
[NMR Sparky Yahoo group] distance constraints from NOESY crosspeaks
distance constraints from NOESY crosspeaks
Hello users, I have a NOESY spectrum of a DNA/RNA strand. I try to get the distance constraints by integration of the NOESY crosspeaks and then converting the
More...
nmrlearner
News from other NMR forums
0
02-10-2012 09:28 PM
[NMR paper] Anomalous diffusion in a gel-fluid lipid environment: a combined solid-state NMR and
Anomalous diffusion in a gel-fluid lipid environment: a combined solid-state NMR and obstructed random-walk perspective.
Related Articles Anomalous diffusion in a gel-fluid lipid environment: a combined solid-state NMR and obstructed random-walk perspective.
Biophys J. 2004 Oct;87(4):2456-69
Authors: Arnold A, Paris M, Auger M
Lateral diffusion is an essential process for the functioning of biological membranes. Solid-state nuclear magnetic resonance (NMR) is, a priori, a well-suited technique to study lateral diffusion within a heterogeneous...
nmrlearner
Journal club
0
11-24-2010 10:01 PM
[NMR paper] Conformational characteristics of high affinity Sp1 binding enhancer elements of HIV-
Conformational characteristics of high affinity Sp1 binding enhancer elements of HIV-LTR by high resolution 2D-NMR.
Related Articles Conformational characteristics of high affinity Sp1 binding enhancer elements of HIV-LTR by high resolution 2D-NMR.
J Biomol Struct Dyn. 1995 Dec;13(3):553-64
Authors: Singh MP, Pon RT, Lown JW
The understanding of early events in the expression of genes has vastly improved in recent years with the identification of a variety of gene- and sequence-specific DNA binding transcription factors. One such protein, Sp1,...
nmrlearner
Journal club
0
08-22-2010 03:50 AM
[NMR paper] Studies on phosphorylated transcriptional regulator (NarL) for E. coli nar operon by
Studies on phosphorylated transcriptional regulator (NarL) for E. coli nar operon by 31P-NMR spectroscopy.
Related Articles Studies on phosphorylated transcriptional regulator (NarL) for E. coli nar operon by 31P-NMR spectroscopy.
Biochem Mol Biol Int. 1993 Sep;31(1):161-8
Authors: Takahashi K, Hattori T, Shindo H, Noji S, Nohno T, Taniguchi S
The sequential transphosphorylation from autophosphorylated nitrate-sensing protein (NarX) to the transcriptional regulator protein (NarL), both operating in signal transduction to control the expression...
nmrlearner
Journal club
0
08-22-2010 03:01 AM
[NMR paper] Structural analyses of CREB-CBP transcriptional activator-coactivator complexes by NM
Structural analyses of CREB-CBP transcriptional activator-coactivator complexes by NMR spectroscopy: implications for mapping the boundaries of structural domains.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Structural analyses of CREB-CBP transcriptional activator-coactivator complexes by NMR spectroscopy: implications for mapping the boundaries of structural domains.
J Mol Biol. 1999 Apr 16;287(5):859-65
Authors: Radhakrishnan I, Pérez-Alvarado GC, Parker D, Dyson HJ,...
nmrlearner
Journal club
0
08-21-2010 04:03 PM
[NMR paper] Identification of the single-stranded DNA binding surface of the transcriptional coac
Identification of the single-stranded DNA binding surface of the transcriptional coactivator PC4 by NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-standard-jbc_full_free.gif Related Articles Identification of the single-stranded DNA binding surface of the transcriptional coactivator PC4 by NMR.
J Biol Chem. 1999 Feb 5;274(6):3693-9
Authors: Werten S, Wechselberger R, Boelens R, van der Vliet PC, Kaptein R
The C-terminal domain of the eukaryotic transcriptional cofactor PC4...
Transcriptional enhancer related DNA sequences: anomalous 1H NMR NOE crosspeaks.
Linear Mode
