[NMR paper] Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a)pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics.
Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a)pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics.
Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a)pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics.
Aquat Toxicol. 2017 Sep 11;192:48-57
Authors: Song Q, Zhou H, Han Q, Diao X
Abstract
Dichlorodiphenyltrichloroethane (DDT) and benzo(a)pyrene (BaP) are environmental estrogens (EEs) that are ubiquitous in the marine environment. In the present study, we integrated isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic and nuclear magnetic resonance (NMR)-based metabolomic approaches to explore the toxic responses of green mussel hepatopancreas exposed to DDT (10?g/L), BaP (10?g/L) and their mixture. The metabolic responses indicated that BaP primarily disturbed energy metabolism and osmotic regulation in the hepatopancreas of the male green mussel P. viridis. Both DDT and the mixture of DDT and BaP perturbed the energy metabolism and osmotic regulation in P. viridis. The proteomic responses revealed that BaP affected the proteins involved in energy metabolism, material transformation, cytoskeleton, stress responses, reproduction and development in green mussels. DDT exposure could change the proteins involved in primary metabolism, stress responses, cytoskeleton and signal transduction. However, the mixture of DDT and BaP altered proteins associated with material and energy metabolism, stress responses, signal transduction, reproduction and development, cytoskeleton and apoptosis. This study showed that iTRAQ-based proteomic and NMR-based metabolomic approaches could effectively elucidate the essential molecular mechanism of disturbances in hepatopancreas function of green mussels exposed to environmental estrogens.
PMID: 28917945 [PubMed - as supplied by publisher]
[NMR paper] Proteomics and (1)H NMR-based metabolomics analysis of pathogenic Vibrio vulnificus aquacultures isolated from sewage drains.
Proteomics and (1)H NMR-based metabolomics analysis of pathogenic Vibrio vulnificus aquacultures isolated from sewage drains.
Related Articles Proteomics and (1)H NMR-based metabolomics analysis of pathogenic Vibrio vulnificus aquacultures isolated from sewage drains.
Environ Sci Pollut Res Int. 2017 Sep 01;:
Authors: Zhang C, Wang Z, Zhang D, Zhou J, Lu C, Su X, Ding D
Abstract
Vibrio bacteria live in both marine and freshwater habitats and are associated with aquatic animals. Vibrio vulnificus is a pathogenic bacterium that...
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09-04-2017 02:06 AM
[NMR paper] Salecan protected against concanavalin A-induced acute liver injury by modulating T cell immune responses and NMR-based metabolic profiles.
Salecan protected against concanavalin A-induced acute liver injury by modulating T cell immune responses and NMR-based metabolic profiles.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Salecan protected against concanavalin A-induced acute liver injury by modulating T cell immune responses and NMR-based metabolic profiles.
Toxicol Appl Pharmacol. 2017 Feb 15;317:63-72
Authors: Sun Q, Xu X, Yang X, Weng D, Wang J, Zhang J
Abstract
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06-01-2017 03:02 PM
[NMR paper] A fast approach to 3D HSQC-based spectroscopy based on a phase-based Fourier encoding of pre-targeted resonances
A fast approach to 3D HSQC-based spectroscopy based on a phase-based Fourier encoding of pre-targeted resonances
Publication date: Available online 21 November 2016
Source:Journal of Magnetic Resonance</br>
Author(s): Yulan Lin, Pieter E S Smith, Zhiyong Zhang, Lucio Frydman</br>
Multidimensional Nuclear Magnetic Resonance (NMR) provides a unique window into structure and dynamics at an atomic level. Traditionally, given the scan-by-scan time modulation involved in these experiments, the duration of nD NMR increases exponentially with spectral dimensionality....
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11-21-2016 11:02 PM
[NMR paper] NMR-based metabonomic analysis of physiological responses to starvation and refeeding in the rat.
NMR-based metabonomic analysis of physiological responses to starvation and refeeding in the rat.
Related Articles NMR-based metabonomic analysis of physiological responses to starvation and refeeding in the rat.
J Proteome Res. 2016 Aug 12;
Authors: Serrano-Contreras JI, Garcia-Perez I, Meléndez-Camargo ME, Zepeda LG
Abstract
Starvation is a postabsorptive condition derived from a limitation on food resources by external factors. Energy homeostasis is maintained under this condition by using sources other than glucose via...
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08-16-2016 07:38 PM
[NMR paper] (1)H NMR based metabolomics approach to study the toxic effects of dichlorvos on goldfish (Carassius auratus).
(1)H NMR based metabolomics approach to study the toxic effects of dichlorvos on goldfish (Carassius auratus).
(1)H NMR based metabolomics approach to study the toxic effects of dichlorvos on goldfish (Carassius auratus).
Chemosphere. 2015 Jul 22;138:537-545
Authors: Liu Y, Chen T, Li MH, Xu HD, Jia AQ, Zhang JF, Wang JS
Abstract
Dichlorvos (DDVP), one of the most widely used organophosphorus pesticides (OPs), has caused serious pollution in environment. In this study, (1)H nuclear magnetic resonance (NMR) based metabolomics...
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07-27-2015 10:16 AM
[U. of Ottawa NMR Facility Blog] Cross Polarization Based Mixture Resolution in Solids
Cross Polarization Based Mixture Resolution in Solids
One of the most common techniques used to collect solid-state NMR data for spin I = ˝ nuclides is a combination of cross polarization (CP) and magic angle spinning (MAS). CPMAS provides high sensitivity from the CP and high chemical shift resolution from the MAS. Furthermore, the scan repetition rate depends on the shorter relaxation time of the protons rather than the longer relaxation time of the spin I = ˝ nuclide therefore, more scans can be collected per unit time. It must be remembered however, that the success of the CP...
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03-31-2015 07:17 PM
[NMR paper] A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
J Invertebr Pathol. 2014 Oct 14;124C:51-56
Authors: Liu PF, Liu QH, Wu Y, Jie H
Abstract
White spot syndrome virus, which was a pathogen first found in 1992, had emerged globally affecting shrimp populations in aquaculture. Here, we comprehensively...
Toxic responses of Perna viridis hepatopancreas exposed to DDT, benzo(a)pyrene and their mixture uncovered by iTRAQ-based proteomics and NMR-based metabolomics.
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