Related ArticlesTowards an integrative structural biology approach: combining Cryo-TEM, X-ray crystallography, and NMR.
J Struct Funct Genomics. 2014 Apr 20;
Authors: Lengyel J, Hnath E, Storms M, Wohlfarth T
Abstract
Cryo-transmission electron microscopy (Cryo-TEM) and particularly single particle analysis is rapidly becoming the premier method for determining the three-dimensional structure of protein complexes, and viruses. In the last several years there have been dramatic technological improvements in Cryo-TEM, such as advancements in automation and use of improved detectors, as well as improved image processing techniques. While Cryo-TEM was once thought of as a low resolution structural technique, the method is currently capable of generating nearly atomic resolution structures on a routine basis. Moreover, the combination of Cryo-TEM and other methods such as X-ray crystallography, nuclear magnetic resonance spectroscopy, and molecular dynamics modeling are allowing researchers to address scientific questions previously thought intractable. Future technological developments are widely believed to further enhance the method and it is not inconceivable that Cryo-TEM could become as routine as X-ray crystallography for protein structure determination.
PMID: 24748171 [PubMed - as supplied by publisher]
The dynamic duo: Combining NMR and small angle scattering in structural biology
The dynamic duo: Combining NMR and small angle scattering in structural biology
Abstract
Structural biology provides essential information for elucidating molecular mechanisms that underlie biological function. Advances in hardware, sample preparation, experimental methods, and computational approaches now enable structural analysis of protein complexes with increasing complexity that more closely represent biologically entities in the cellular environment. Integrated multidisciplinary approaches are required to overcome limitations of individual methods and take advantage of...
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[NMR paper] The Dynamic Duo: Combining NMR and Small Angle Scattering in Structural Biology.
The Dynamic Duo: Combining NMR and Small Angle Scattering in Structural Biology.
The Dynamic Duo: Combining NMR and Small Angle Scattering in Structural Biology.
Protein Sci. 2014 Mar 29;
Authors: Hennig J, Sattler M
Abstract
Structural biology provides essential information for elucidating molecular mechanisms that underlie biological function. Advances in hardware, sample preparation, experimental methods and computational approaches now enable structural analysis of protein complexes with increasing complexity that more...
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04-02-2014 11:54 PM
The Dynamic Duo: Combining NMR and Small Angle Scattering in Structural Biology
The Dynamic Duo: Combining NMR and Small Angle Scattering in Structural Biology
Abstract
Structural biology provides essential information for elucidating molecular mechanisms that underlie biological function. Advances in hardware, sample preparation, experimental methods and computational approaches now enable structural analysis of protein complexes with increasing complexity that more closely represent biologically entities in the cellular environment. Integrated multi-disciplinary approaches are required to overcome limitations of individual methods and take advantage of...
Scientists at UCSF Set New Cryo EM Resolution Benchmark in Structural Biology - GlobeNewswire (press release)
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Scientists at UCSF Set New Cryo EM Resolution Benchmark in Structural Biology
GlobeNewswire (press release)
Most atomic resolution protein structures come from X-ray crystallography, a technique that is limited to samples which can be crystalized, a challenging task that can sometimes take years of effort, and from nuclear magnetic resonance (NMR ...
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Scientists at UCSF Set New Cryo EM Resolution Benchmark in Structural Biology - GlobeNewswire (press release)
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05-07-2013 12:31 AM
Research Scientist Position in the Cryo-Electron Microscopy Facility at the New York Structural Biol - New York Structural Biology Center - New York, NY, United States
Research Scientist Position in the Cryo-Electron Microscopy Facility at the New York Structural Biol - New York Structural Biology Center - New York, NY, United States
The New York Structural Biology Center (NYSBC) seeks an experienced electron microscopist to join the staff of its Cryo-Electron Microscope Facility (http://cryoem.nysbc.org). The NYSBC is shared center that supports state-of-the-art research in cryo-EM, NMR, and X-ray. Cryo-EM facilities include four transmission electron microscopes and a new d...
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[NMR paper] A novel approach for assessing macromolecular complexes combining soft-docking calcul
A novel approach for assessing macromolecular complexes combining soft-docking calculations with NMR data.
Related Articles A novel approach for assessing macromolecular complexes combining soft-docking calculations with NMR data.
Protein Sci. 2001 Oct;10(10):2131-7
Authors: Morelli XJ, Palma PN, Guerlesquin F, Rigby AC
We present a novel and efficient approach for assessing protein-protein complex formation, which combines ab initio docking calculations performed with the protein docking algorithm BiGGER and chemical shift perturbation data...
Towards an integrative structural biology approach: combining Cryo-TEM, X-ray crystallography, and NMR.
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