Nuclear magnetic resonance (NMR) spectroscopy is a key method for determining the structural dynamics of proteins in their native solution state. However, the low sensitivity of NMR typically necessitates nonphysiologically high sample concentrations, which often limit the relevance of the recorded data. We show how to use hyperpolarized water by dissolution dynamic nuclear polarization (DDNP) to acquire protein spectra at concentrations of 1 ?M within seconds and with a high signal-to-noise...
[NMR paper] High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents.
High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents.
Related Articles High-Resolution NMR of Folded Proteins in Hyperpolarized Physiological Solvents.
Chemistry. 2018 Jul 03;:
Authors: Kaderavek P, Ferrage F, Bodenhausen G, Kurzbach D
Abstract
We report hyperpolarized 2D exchange spectroscopy (HYPEX) to obtain high-resolution nuclear magnetic resonance (NMR) spectra of folded proteins under near-physiological conditions. The technique is based on hyperpolarized water, which is prepared by dissolution...
nmrlearner
Journal club
0
07-06-2018 09:40 AM
Hyperpolarized Water to Study Protein–Ligand Interactions
From The DNP-NMR Blog:
Hyperpolarized Water to Study Protein–Ligand Interactions
Chappuis, Q., et al., Hyperpolarized Water to Study Protein–Ligand Interactions. The Journal of Physical Chemistry Letters, 2015. 6(9): p. 1674-1678.
http://dx.doi.org/10.1021/acs.jpclett.5b00403
nmrlearner
News from NMR blogs
0
06-06-2015 12:01 PM
[NMR paper] Mapping the population of protein conformational energy sub-States from NMR dipolar couplings.
From Mendeley Biomolecular NMR group:
Mapping the population of protein conformational energy sub-States from NMR dipolar couplings.
Angewandte Chemie (International ed. in English) (2013). Volume: 52, Issue: 11. Pages: 3181-5. Paul Guerry, Loïc Salmon, Luca Mollica, Jose-Luis Ortega Roldan, Phineus Markwick, Nico a J van Nuland, J Andrew McCammon, Martin Blackledge et al.
Molecular dynamics: A general method for the statistical mechanical description of conformational energy landscapes of proteins in solution is proposed. This method combines NMR residual dipolar couplings (RDCs),...
nmrlearner
Journal club
0
10-17-2013 12:49 PM
[NMR paper] Mapping Protein Conformational Energy Landscapes Using NMR and Molecular Simulation.
Mapping Protein Conformational Energy Landscapes Using NMR and Molecular Simulation.
Mapping Protein Conformational Energy Landscapes Using NMR and Molecular Simulation.
Chemphyschem. 2013 May 23;
Authors: Guerry P, Mollica L, Blackledge M
Abstract
Nuclear magnetic resonance (NMR) spectroscopy provides detailed understanding of the nature and extent of protein dynamics on physiologically important timescales. We present recent advances in the combination of NMR with state-of-the-art molecular simulation that are providing unique new...
nmrlearner
Journal club
0
05-25-2013 12:05 PM
[NMR paper] Mapping the population of protein conformational energy sub-States from NMR dipolar couplings.
From Mendeley Biomolecular NMR group:
Mapping the population of protein conformational energy sub-States from NMR dipolar couplings.
Angewandte Chemie (International ed. in English) (2013). Volume: 52, Issue: 11. Pages: 3181-5. Paul Guerry, Loïc Salmon, Luca Mollica, Jose-Luis Ortega Roldan, Phineus Markwick, Nico a J van Nuland, J Andrew McCammon, Martin Blackledge et al.
Molecular dynamics: A general method for the statistical mechanical description of conformational energy landscapes of proteins in solution is proposed. This method combines NMR residual dipolar couplings (RDCs),...
nmrlearner
Journal club
0
04-11-2013 03:08 PM
[NMR paper] Mapping the Population of Protein Conformational Energy Sub-States from NMR Dipolar Couplings.
Mapping the Population of Protein Conformational Energy Sub-States from NMR Dipolar Couplings.
Related Articles Mapping the Population of Protein Conformational Energy Sub-States from NMR Dipolar Couplings.
Angew Chem Int Ed Engl. 2013 Feb 1;
Authors: Guerry P, Salmon L, Mollica L, Ortega Roldan JL, Markwick P, van Nuland NA, McCammon JA, Blackledge M
Abstract
Molecular dynamics: A general method for the statistical mechanical description of conformational energy landscapes of proteins in solution is proposed. This method combines NMR...
nmrlearner
Journal club
0
02-03-2013 10:19 AM
Liquid State DNP for Water Accessibility Measurements on Spin-labeled Membrane Proteins at Physiological Temperatures
Liquid State DNP for Water Accessibility Measurements on Spin-labeled Membrane Proteins at Physiological Temperatures
Publication year: 2012
Source:Journal of Magnetic Resonance</br>
Andrin Doll, Enrica Bordignon, Benesh Joseph, René Tschaggelar, Gunnar Jeschke</br>
We demonstrate the application of continuous wave dynamic nuclear polarization (DNP) at 0.35 Tesla for site-specific water accessibility studies on spin-labeled membrane proteins at concentrations in the 10-100 micromolar range. The DNP effects at such low concentrations are weak and the experimentally...
nmrlearner
Journal club
0
06-16-2012 06:01 AM
[Question from NMRWiki Q&A forum] Which are better methods of water suppression at higher salt concentrations?
Which are better methods of water suppression at higher salt concentrations?
Hello, could anyone recommend better technique for water suppression for the samples having higher salt concentration?
At what salt concentration is the method effective?
Dr. Talluri mentioned in his post that water suppression using soft pulses (such as one using excitation sculpting) is not as friendly for high salt samples - what is the mechanism?
Toward protein NMR at physiological concentrations by hyperpolarized water-Finding and mapping uncharted conformational spaces
Linear Mode
