Related ArticlesThree-dimensional electrophoretic NMR correlation spectroscopy.
J Magn Reson. 2000 Dec;147(2):361-5
Authors: He Q, Lin W, Liu Y, Li E
A novel method of three-dimensional electrophoretic NMR correlation spectroscopy (3D EP-COSY) has been proposed, developed, and implemented. It has a demonstrated potential of facilitating simultaneous structural assignments of multiple proteins in mixtures. The principle is to add a pulsed DC electric field that introduces a new dimension of electrophoretic flow, in which resonances of different molecules can be separated by their electrophoretic migration rates without physical separation. As a result, two COSY spectra were simultaneously obtained in a single 3D EP-COSY experiment from a mixture of 150 mM l-aspartic acid and 148 mM 4, 9-dioxa-1,12-dodecanediamine with concurrent resolution of their chemical shifts and J-coupling constants. This approach creates a new horizon of multidimensional electrophoretic NMR. The technical advance opens doors for structure characterization of complex protein systems and protein interactions, which are at the basis of biochemical mechanisms and the phenomena of living systems.
Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins
Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins
Abstract Well-resolved 2Hâ??13C correlation spectra, reminiscent of 1Hâ??13C correlations, are obtained for perdeuterated ubiquitin and for perdeuterated outer-membrane protein G (OmpG) from E. coli by exploiting the favorable lifetime of 2H double-quantum (DQ) states. Sufficient signal-to-noise was achieved due to the short deuterium T 1, allowing for high repetition rates and enabling 3D experiments with a 2Hâ??13C transfer step in a reasonable time....
Characteristics of zero-quantum correlation spectroscopy in MAS NMR experiments.
Characteristics of zero-quantum correlation spectroscopy in MAS NMR experiments.
Characteristics of zero-quantum correlation spectroscopy in MAS NMR experiments.
J Magn Reson. 2010 Dec;207(2):197-205
Authors: Köneke SG, van Beek JD, Ernst M, Meier BH
Zero-quantum coherence generation and reconversion in magic-angle spinning solid-state NMR is analyzed. Two methods are discussed based on implementations using symmetry-based pulse sequences that utilize either isotropic J couplings or dipolar couplings. In either case, the decoupling of abundant...
nmrlearner
Journal club
0
03-18-2011 06:00 PM
Spin Diffusion Driven by R-Symmetry Sequences: Applications to Homonuclear Correlation Spectroscopy in MAS NMR of Biological and Organic Solids
Spin Diffusion Driven by R-Symmetry Sequences: Applications to Homonuclear Correlation Spectroscopy in MAS NMR of Biological and Organic Solids
Guangjin Hou, Si Yan, Shangjin Sun, Yun Han, In-Ja L. Byeon, Jinwoo Ahn, Jason Concel, Ago Samoson, Angela M. Gronenborn and Tatyana Polenova
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja108650x/aop/images/medium/ja-2010-08650x_0001.gif
Journal of the American Chemical Society
DOI: 10.1021/ja108650x
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA...
nmrlearner
Journal club
0
03-02-2011 02:01 AM
[NMR paper] Constant-time multidimensional electrophoretic NMR.
Constant-time multidimensional electrophoretic NMR.
Related Articles Constant-time multidimensional electrophoretic NMR.
J Magn Reson. 2002 Jun;156(2):181-6
Authors: Li E, He Q
Multidimensional electrophoretic NMR (ENMR) has been introduced to determine structures of coexisting proteins and protein conformations in solution. Signals of different proteins are separated in a new dimension of electrophoretic flow according to their characteristic electrophoretic mobilities. The electrophoretic interferograms have been generated in the flow...
nmrlearner
Journal club
0
11-24-2010 08:49 PM
[NMR paper] Convection compensated electrophoretic NMR.
Convection compensated electrophoretic NMR.
Related Articles Convection compensated electrophoretic NMR.
J Magn Reson. 2001 Jun;150(2):126-31
Authors: He Q, Wei Z
A novel method of convection compensated ENMR (CC-ENMR) has been developed to detect electrophoretic motion of ionic species in the presence of bulk solution convection. This was accomplished using a gradient moment nulling technique to remove spectral artifacts from heat-induced convection and using the polarity switch of the applied electric field to retain spin phase modulations...
nmrlearner
Journal club
0
11-19-2010 08:32 PM
[NMR paper] Optimization of three-dimensional TROSY-type HCCH NMR correlation of aromatic (1)H-(1
Optimization of three-dimensional TROSY-type HCCH NMR correlation of aromatic (1)H-(13)C groups in proteins.
Related Articles Optimization of three-dimensional TROSY-type HCCH NMR correlation of aromatic (1)H-(13)C groups in proteins.
J Magn Reson. 1999 Aug;139(2):447-50
Authors: Meissner A, Sorensen OW
Improved methods for three-dimensional TROSY-Type HCCH correlation involving protons of negligible CSA are presented. The TROSY approach differs from the conventional approach of heteronuclear decoupling in evolution and detection periods by...
nmrlearner
Journal club
0
11-18-2010 08:31 PM
Four-dimensional heteronuclear correlation experiments for chemical shift assignment of solid proteins
Four-dimensional heteronuclear correlation experiments for chemical shift assignment of solid proteins
W. Trent Franks, Kathryn D. Kloepper, Benjamin J. Wylie and Chad M. Rienstra
Journal of Biomolecular NMR; 2007; 39(2); pp 107 - 131
Abstract:
Chemical shift assignment is the first step in all established protocols for structure determination of uniformly labeled proteins by NMR. The explosive growth in recent years of magic-angle spinning (MAS) solid-state NMR (SSNMR) applications is largely attributable to improved methods for backbone and side-chain chemical shift correlation...
Three-dimensional electrophoretic NMR correlation spectroscopy.
Linear Mode
