BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > Educational resources > Journal club
Advanced Search
Home Forums Wiki NMR feeds Downloads Register Today's Posts



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
 
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Old 08-21-2010, 11:16 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,776
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Three-dimensional correlated NMR study of Megasphaera elsdenii flavodoxin in the oxid

Three-dimensional correlated NMR study of Megasphaera elsdenii flavodoxin in the oxidized state.

Related Articles Three-dimensional correlated NMR study of Megasphaera elsdenii flavodoxin in the oxidized state.

Eur J Biochem. 1991 Feb 14;195(3):807-22

Authors: Wijmenga SS, van Mierlo CP

The value of a three-dimensional (3D) non-selective total correlation/nuclear Overhauser enhancement spectroscopy (TOCSY-NOESY) spectrum for making sequential resonance assignments in proteins is demonstrated using the relatively large Megasphaera elsdenii flavodoxin (molecular mass 15 kDa) in the oxidized state. An easy and concise method for the analysis of 3D-NMR spectra and a strategy for the resonance assignment of 3D-NMR protein spectra is introduced. In this context, non-selective TOCSY-NOESY is compared with selective TOCSY-NOESY and non-selective NOESY-TOCSY. Sequential assignments in various secondary structure elements of flavodoxin are made using the method of analysis introduced. NOEs not previously identified in 2D-NMR spectra due to resonance overlap are found in the 3D Clean-TOCSY-NOESY spectrum. Also additional side-chain assignments could be made.

PMID: 1999199 [PubMed - indexed for MEDLINE]



Source: PubMed
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
How uniform is the peptide plane geometry? A high-accuracy NMR study of dipolar Cαâ??Câ?²/HNâ??N cross-correlated relaxation
How uniform is the peptide plane geometry? A high-accuracy NMR study of dipolar Cαâ??Câ?²/HNâ??N cross-correlated relaxation Abstract Highly precise and accurate measurements of very small NMR cross-correlated relaxation rates, namely those between protein HiNâ??Ni and Ciâ??1αâ??Ciâ??1â?² dipoles, are demonstrated with an error of 0.03 sâ??1 for GB3. Because the projection angles between the two dipole vectors are very close to the magic angle the rates range only from â??0.2 to +0.2 sâ??1. Small changes of the average vector orientations have a dramatic impact on the relative values....
nmrlearner Journal club 0 06-06-2011 12:53 AM
How uniform is the peptide plane geometry? A high-accuracy NMR study of dipolar C(?)-C'/H (N)-N cross-correlated relaxation.
How uniform is the peptide plane geometry? A high-accuracy NMR study of dipolar C(?)-C'/H (N)-N cross-correlated relaxation. How uniform is the peptide plane geometry? A high-accuracy NMR study of dipolar C(?)-C'/H (N)-N cross-correlated relaxation. J Biomol NMR. 2011 Jun 3; Authors: Vögeli B Highly precise and accurate measurements of very small NMR cross-correlated relaxation rates, namely those between protein H (i) (N) -N(i) and C (i-1) (?) -C(i-1)' dipoles, are demonstrated with an error of 0.03*s(-1) for GB3. Because the projection angles...
nmrlearner Journal club 0 06-04-2011 11:26 AM
[NMR paper] Disulfide bond isomerization in BPTI and BPTI(G36S): an NMR study of correlated mobil
Disulfide bond isomerization in BPTI and BPTI(G36S): an NMR study of correlated mobility in proteins. Related Articles Disulfide bond isomerization in BPTI and BPTI(G36S): an NMR study of correlated mobility in proteins. Biochemistry. 1993 Apr 13;32(14):3571-82 Authors: Otting G, Liepinsh E, Wüthrich K Two conformational isomers were observed in the 1H nuclear magnetic resonance (NMR) spectra of the basic pancreatic trypsin inhibitor (BPTI) and of a mutant protein with Gly 36 replaced by Ser, BPTI(G36S). The less abundant isomer differs from...
nmrlearner Journal club 0 08-21-2010 11:53 PM
[NMR paper] NOE and two-dimensional correlated 1H-NMR spectroscopy of cytochrome c' from Chromati
NOE and two-dimensional correlated 1H-NMR spectroscopy of cytochrome c' from Chromatium vinosum. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles NOE and two-dimensional correlated 1H-NMR spectroscopy of cytochrome c' from Chromatium vinosum. Eur J Biochem. 1992 Feb 15;204(1):107-12 Authors: Banci L, Bertini I, Turano P, Vicens Oliver M 1H two-dimensional (nuclear Overhauser effect spectroscopy (NOESY) and two-dimensional...
nmrlearner Journal club 0 08-21-2010 11:41 PM
[NMR paper] A two-dimensional 1H-NMR study on Megasphaera elsdenii flavodoxin in the oxidized sta
A two-dimensional 1H-NMR study on Megasphaera elsdenii flavodoxin in the oxidized state and some comparisons with the two-electron-reduced state. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles A two-dimensional 1H-NMR study on Megasphaera elsdenii flavodoxin in the oxidized state and some comparisons with the two-electron-reduced state. Eur J Biochem. 1990 Nov 26;194(1):199-216 Authors: van Mierlo CP, van der Sanden BP, van Woensel P,...
nmrlearner Journal club 0 08-21-2010 11:04 PM
[NMR paper] Tertiary structure of two-electron reduced Megasphaera elsdenii flavodoxin and some i
Tertiary structure of two-electron reduced Megasphaera elsdenii flavodoxin and some implications, as determined by two-dimensional 1H-NMR and restrained molecular dynamics. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Tertiary structure of two-electron reduced Megasphaera elsdenii flavodoxin and some implications, as determined by two-dimensional 1H-NMR and restrained molecular dynamics. Eur J Biochem. 1990 Nov 26;194(1):185-98 Authors: ...
nmrlearner Journal club 0 08-21-2010 11:04 PM
[NMR paper] Secondary and tertiary structure characteristics of Megasphaera elsdenii flavodoxin i
Secondary and tertiary structure characteristics of Megasphaera elsdenii flavodoxin in the reduced state as determined by two-dimensional 1H NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Secondary and tertiary structure characteristics of Megasphaera elsdenii flavodoxin in the reduced state as determined by two-dimensional 1H NMR. Eur J Biochem. 1990 May 20;189(3):589-600 Authors: van Mierlo CP, Müller F, Vervoort J The...
nmrlearner Journal club 0 08-21-2010 10:48 PM
[NMR paper] A two-dimensional 1H NMR study on Megasphaera elsdenii flavodoxin in the reduced stat
A two-dimensional 1H NMR study on Megasphaera elsdenii flavodoxin in the reduced state. Sequential assignments. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles A two-dimensional 1H NMR study on Megasphaera elsdenii flavodoxin in the reduced state. Sequential assignments. Eur J Biochem. 1990 Feb 14;187(3):521-41 Authors: van Mierlo CP, Vervoort J, Müller F, Bacher A Assignments for the 137 amino acid residues of Megasphaera...
nmrlearner Journal club 0 08-21-2010 10:48 PM



Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 02:06 AM.


Map