[NMR paper] A bioresistant nitroxide spin label for in-cell EPR spectroscopy: in vitro and in oocytes protein structural dynamics studies.
A bioresistant nitroxide spin label for in-cell EPR spectroscopy: in vitro and in oocytes protein structural dynamics studies.
Approaching proteins structural dynamics and protein-protein interactions in the cellular environment is a fundamental challenge. Due to its absolute sensitivity and to its selectivity to paramagnetic species, Site-Directed Spin Labeling (SDSL) combined with Electron Paramagnetic Resonance (EPR) has the potential to evolve into an efficient method to follow conformational changes in proteins directly inside cells. Until now, the use of nitroxyde-based spin labels...
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12-12-2017 02:12 AM
[NMR paper] Potent antidiuretic agonists, deamino-vasopressin and desmopressin, and their inverso analogs: NMR structure and interactions with micellar and liposomic models of cell membrane.
Potent antidiuretic agonists, deamino-vasopressin and desmopressin, and their inverso analogs: NMR structure and interactions with micellar and liposomic models of cell membrane.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles Potent antidiuretic agonists, deamino-vasopressin and desmopressin, and their inverso analogs: NMR structure and interactions with micellar and liposomic models of cell membrane.
Biopolymers. 2016 May;106(3):245-59
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[NMR paper] Arginine-, D-arginine-vasopressin, and their inverso analogues in micellar and liposomic models of cell membrane: CD, NMR, and molecular dynamics studies.
Arginine-, D-arginine-vasopressin, and their inverso analogues in micellar and liposomic models of cell membrane: CD, NMR, and molecular dynamics studies.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Arginine-, D-arginine-vasopressin, and their inverso analogues in micellar and liposomic models of cell membrane: CD, NMR, and molecular dynamics...
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08-31-2016 02:34 PM
[NMR paper] Strategy for NMR metabolomic analysis of urine in mouse models of obesity- from sample collection to interpretation of acquired data.
Strategy for NMR metabolomic analysis of urine in mouse models of obesity- from sample collection to interpretation of acquired data.
Strategy for NMR metabolomic analysis of urine in mouse models of obesity- from sample collection to interpretation of acquired data.
J Pharm Biomed Anal. 2015 Jul 17;115:225-235
Authors: Pelantová H, Bugá?ová M, Anýž J, Železná B, Maletínská L, Novák D, Haluzík M, Kuzma M
Abstract
The mouse model of monosodium glutamate induced obesity was used to examine and consequently optimize the strategy...
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[NMR paper] (1)H NMR spectroscopic studies on the characterization of renal cell lines and identification of novel potential markers of in vitro nephrotoxicity.
(1)H NMR spectroscopic studies on the characterization of renal cell lines and identification of novel potential markers of in vitro nephrotoxicity.
Related Articles (1)H NMR spectroscopic studies on the characterization of renal cell lines and identification of novel potential markers of in vitro nephrotoxicity.
Biomarkers. 1996;1(1):35-43
Authors: Anthony ML, McDowell PC, Gray TJ, Blackmore M, Nicholson JK
Abstract
Abstract Cell cultures are increasingly used in the evaluation of chemically-induced nephrotoxicity. The utili of renal cell...
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07-31-2013 12:00 PM
[U. of Ottawa NMR Facility Blog] Thermal Noise in NMR Data
Thermal Noise in NMR Data
The University of Ottawa has recently been funded for a 600 MHz NMR spectrometer with a cryogenetically cooled probe. Cryoprobes differ from conventional NMR probes in that the rf circuits and preamplifiers are cooled with cold helium gas while the sample is maintained at ambient temperature. The benefit of cryogenically cooled electronics compared to room temperature electronics is that the thermal noise in the system is reduced at cryogenic temperatures while the NMR signal remains constant for the sample at ambient temperature. The signal-to-noise ratio in...
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03-08-2013 08:20 AM
[NMR paper] Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Quantitative comparison of protein dynamics in live cells and in vitro by in-cell (19)F-NMR.
Chem Commun (Camb). 2013 Feb 26;
Authors: Takaoka Y, Kioi Y, Morito A, Otani J, Arita K, Ashihara E, Ariyoshi M, Tochio H, Shirakawa M, Hamachi I
Abstract
Here we describe how a (19)F-probe incorporated into an endogenous protein by a chemical biology method revealed protein dynamics. By explicit determination of ligand-bound and unbound structures with...
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02-27-2013 06:47 PM
Evaluation of competing J domain:Hsp70 complex models in light of existing mutational and NMR data [Letters (Online Only)]
Evaluation of competing J domain:Hsp70 complex models in light of existing mutational and NMR data
Sousa, R., Jiang, J., Lafer, E. M., Hinck, A. P., Wang, L., Taylor, A. B., Maes, E. G....
Date: 2012-03-27
The work by Ahmad et al. (1) presented an NMR-based model for a bacterial DnaJ J domain:DnaK(Hsp70):ADP complex that differs from our crystal structure of a disulfide-linked bovine Hsc70:auxilin J domain complex (2). The work by Ahmad et al. (1) claimed that their model can better account for published mutational data, that their model is in better agreement with a previous NMR...
Thermal and pH stabilities of i-DNA: confronting in vitro experiments with models and in-cell NMR data
Linear Mode
