Publication date: Available online 2 July 2015 Source:Progress in Nuclear Magnetic Resonance Spectroscopy
Author(s): Nicola Salvi
Recent decades have witnessed tremendous progress in the development of new experimental methods for studying biomolecules, particularly in the field of NMR relaxation dispersion. Here we review the theoretical frameworks that provided the insights necessary for such progress. The effect of radio-frequency manipulations on spin systems is discussed using Average Hamiltonian Theory (AHT), Average Liouvillian Theory (ALT), and Bloch-Wangsness-Redfield (BWR) relaxation theory. We illustrate these concepts using the case of Heteronuclear Double Resonance (HDR) methods. Graphical abstract
[NMR paper] A 15N CPMG relaxation dispersion experiment more resistant to resonance offset and pulse imperfection
A 15N CPMG relaxation dispersion experiment more resistant to resonance offset and pulse imperfection
Publication date: Available online 21 May 2015
Source:Journal of Magnetic Resonance</br>
Author(s): Bin Jiang , Binhan Yu , Xu Zhang , Maili Liu , Daiwen Yang</br>
Carr-Purcell Meiboom-Gill (CPMG) relaxation dispersion is a powerful NMR method to study protein dynamics on the microsecond-millisecond time scale. J-coupling, resonance offset and pulse imperfection often introduce systematic errors into the measured transverse relaxation rates. Here we proposed a...
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05-21-2015 04:28 PM
[Question from NMRWiki Q&A forum] Software to simulate pulse sequences
Software to simulate pulse sequences
Hi,I'm interested in software for windows or linux that simulate the effect of pulses, delay AND gradients using the product operator formalism.Do you have suggestions?ThanksMarco
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06-18-2014 04:23 AM
[NMR paper] NMR spectroscopy tools for structure-aided drug design.
NMR spectroscopy tools for structure-aided drug design.
Related Articles NMR spectroscopy tools for structure-aided drug design.
Angew Chem Int Ed Engl. 2004 Jan 3;43(3):290-300
Authors: Homans SW
Biomolecular NMR spectroscopy has expanded dramatically in recent years and is now a powerful tool for the study of structure, dynamics, and interactions of biomolecules. Previous limitations with respect to molecular size are no longer a primary barrier, and systems as large as 900 kDa were recently studied. NMR spectroscopy is already...
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Journal club
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11-24-2010 09:25 PM
Oligo design tools for gene synthesis
Oligo design tools for gene synthesis
1 DNAWorks: http://helixweb.nih.gov/dnaworks/
2 TmPrime: http://prime.ibn.a-star.edu.sg/
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Proteins
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08-25-2010 04:37 AM
[Question from NMRWiki Q&A forum] How do you port pulse sequences to different field strength?
How do you port pulse sequences to different field strength?
Hi, when you set up a pulse sequence at a different magnet field strength - starting with an existing set of parameters - what do you look at?
Does length of shaped pulses always need to be changed?
What pulse lenghts need to be adjusted?
When it is necessary to re-build RF waveforms - for what kinds of pulses?
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08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] 2D pulse sequences for NMR of protein/peptide complexes?
2D pulse sequences for NMR of protein/peptide complexes?
Hello, I am looking for some 15N/13C-filtered 2D NOESY and 2D TOCSY pulse sequences to run on a Varian 600. Does anyone have any ideas where I would get some tried and tested pulse sequence input files?
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08-22-2010 02:30 AM
BMRB library of Bruker pulse sequences
The following Bruker pulse sequences can be found on BMRB website.
Shift correlation:
Homonuclear
Three-dimensional TOCSY-HSQC 3D experiment
NOESY-HSQC 3D experiment
Heteronuclear
nmrlearner
NMR pulse sequences
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03-26-2005 06:18 AM
NMR pulse sequences of Danish Instrument Center
The following NMR pulse sequences for biological macromolecules are available on the website of the Danish Instrument Center as of 3/15/05: Coupling Constants in Proteins
- 2D S3CT for measurement of one bond coupling constants (J & RDC) - Varian, Bruker
TROSY experiments
- clean TROSY, 15N-1H correlation, gradient version, water flip back, optional t2 decoupling - Varian, Bruker
- constant-time S3CT TROSY, 13 C-1H correlation, gradient version - Varian, Bruker
- (H)CCH TROSY, 3D TROSY-type HCCH correlation with diagonal peak suppression - Varian
- TROSY-Type NOESY, 2D NOESY with...
Theoretical Tools for the Design of NMR Relaxation Dispersion Pulse Sequences
Linear Mode
