Related ArticlesTFIIA-TAF regulatory interplay: NMR evidence for overlapping binding sites on TBP.
FEBS Lett. 2000 Feb 25;468(2-3):149-54
Authors: Bagby S, Mal TK, Liu D, Raddatz E, Nakatani Y, Ikura M
TATA box binding protein (TBP)-promoter interaction nucleates assembly of the RNA polymerase II transcription initiation complex. Transcription factor IIA (TFIIA) stabilizes the TBP-promoter complex whereas the N-terminal domain of the largest TAF(II) inhibits TBP-promoter interaction. We have mapped the interaction sites on TBP of Drosophila TAF(II)230 and yeast TFIIA (comprising two subunits, TOA1 and TOA2), using nuclear magnetic resonance (NMR), and also report structural evidence that subdomain II of the TAF(II)230 N-terminal inhibitory domain and TFIIA have overlapping binding sites on the convex surface of TBP. Together with previous mutational and biochemical data, our NMR results indicate that subdomain II augments subdomain I-mediated inhibition of TBP function by blocking TBP-TFIIA interaction.
[NMR paper] Determination of regulatory phosphorylation sites in nanogram amounts of a synthetic fragment of ZAP-70 using microprobe NMR and on-line coupled capillary HPLC-NMR.
Determination of regulatory phosphorylation sites in nanogram amounts of a synthetic fragment of ZAP-70 using microprobe NMR and on-line coupled capillary HPLC-NMR.
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Magn Reson Chem. 2005 Sep;43(9):747-54
Authors: Hentschel P, Krucker M, Grynbaum MD, Putzbach K, Bischoff R, Albert K
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[NMR paper] NMR evidence for similarities between the DNA-binding regions of Drosophila melanogas
NMR evidence for similarities between the DNA-binding regions of Drosophila melanogaster heat shock factor and the helix-turn-helix and HNF-3/forkhead families of transcription factors.
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Biochemistry. 1994 Jan 11;33(1):10-6
Authors: Vuister GW, Kim SJ, Wu C, Bax A
Heteronuclear multidimensional NMR experiments of residues 33-163 of the...
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[NMR paper] 19F NMR evidence for interactions between the c-AMP binding sites on the c-AMP recept
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FEBS Lett. 1991 May 20;283(1):127-30
Authors: Hinds MG, King RW, Feeney J
The 19F NMR spectra of 3-fluorotyrosine containing c-AMP receptor protein (CRP) from E. coli have been recorded in the presence of...
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[NMR paper] The location of the polyphosphate-binding sites on cytochrome c measured by NMR param
The location of the polyphosphate-binding sites on cytochrome c measured by NMR paramagnetic difference spectroscopy.
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Eur J Biochem. 1991 Aug 1;199(3):569-74
Authors: Concar DW, Whitford D, Williams RJ
Analyses of unimolecular electron self-exchange reactions provide...
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[NMR paper] The location of the polyphosphate-binding sites on cytochrome c measured by NMR param
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Eur J Biochem. 1991 Aug 1;199(3):569-74
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[NMR paper] 19F NMR study of the myosin and tropomyosin binding sites on actin.
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Biochemistry. 1990 Feb 6;29(5):1348-54
Authors: Barden JA, Phillips L
Actin was labeled with pentafluorophenyl isothiocyanate at Lys-61. The label was sufficiently small not to affect the rate or extent of actin polymerization unlike the much larger fluorescein 5-isothiocyanate which completely inhibits actin polymerization . Furthermore, the label resonances in the 376.3-MHz 19F NMR spectrum...
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[NMR paper] Identification of the ribosome binding sites of translation initiation factor IF3 by
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RNA. 1999 Jan;5(1):82-92
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Titrations of Escherichia coli translation initiation...
TFIIA-TAF regulatory interplay: NMR evidence for overlapping binding sites on TBP.
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