NMR paper Taylor Dispersion-Induced Phase Separation for the Efficient Characterisation of Protein Condensate Formation

		BioNMR > Educational resources > Journal club
[NMR paper] Taylor Dispersion-Induced Phase Separation for the Efficient Characterisation of Protein Condensate Formation

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

05-15-2024, 08:33 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Taylor Dispersion-Induced Phase Separation for the Efficient Characterisation of Protein Condensate Formation

Taylor Dispersion-Induced Phase Separation for the Efficient Characterisation of Protein Condensate Formation

Angewandte Chemie International Edition, EarlyView.

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy - Nature.com Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy - Nature.com Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy Nature.com Read here	nmrlearner	Online News	0	03-30-2024 09:22 AM
[NMR paper] Fibrils Emerging from Droplets: Molecular Guiding Principles behind Phase Transitions of a Short Peptide-Based Condensate Studied by Solid-State NMR Fibrils Emerging from Droplets: Molecular Guiding Principles behind Phase Transitions of a Short Peptide-Based Condensate Studied by Solid-State NMR Biochemical reactions occurring in highly crowded cellular environments require different means of control to ensure productivity and specificity. Compartmentalization of reagents by liquid-liquid phase separation is one of these means. However, extremely high local protein concentrations of up to 400 mg/ml can result in pathological aggregation into fibrillar amyloid structures, a phenomenon that has been linked to various neurodegenerative...	nmrlearner	Journal club	0	06-14-2023 10:14 PM
Contributions of the N-terminal intrinsically disordered region of the severe acute respiratory syndrome coronavirus 2 nucleocapsid protein to RNA-induced phase separation - Wiley Contributions of the N-terminal intrinsically disordered region of the severe acute respiratory syndrome coronavirus 2 nucleocapsid protein to RNA-induced phase separation - Wiley Contributions of the N-terminal intrinsically disordered region of the severe acute respiratory syndrome coronavirus 2 nucleocapsid protein to RNA-induced phase separation Wiley Read here	nmrlearner	Online News	0	10-20-2022 06:37 PM
Contributions of the N-terminal intrinsically disordered region of the severe acute respiratory syndrome coronavirus 2 nucleocapsid protein to RNA-induced phase separation - Wiley Contributions of the N-terminal intrinsically disordered region of the severe acute respiratory syndrome coronavirus 2 nucleocapsid protein to RNA-induced phase separation - Wiley Contributions of the N-terminal intrinsically disordered region of the severe acute respiratory syndrome coronavirus 2 nucleocapsid protein to RNA-induced phase separation Wiley Read here	nmrlearner	Online News	0	08-19-2022 10:43 PM
[ASAP] Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences https://pubs.acs.org/cms/10.1021/acs.biochem.2c00250/asset/images/medium/bi2c00250_0006.gif Biochemistry DOI: 10.1021/acs.biochem.2c00250 More...	nmrlearner	Journal club	0	08-04-2022 01:03 AM
[NMR paper] Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy Temporal and spatial characterisation of protein liquid-liquid phase separation using NMR spectroscopy Liquid-liquid phase separation (LLPS) of protein solutions is increasingly recognised as an important phenomenon in cell biology and biotechnology. However, opalescence and concentration fluctuations render LLPS difficult to study, particularly when characterising the kinetics of the phase transition and layer separation. Here, we demonstrate the use of a probe molecule trifluoroethanol (TFE) to characterise the kinetics of protein LLPS by NMR spectroscopy. The chemical shift and...	nmrlearner	Journal club	0	04-03-2022 03:57 PM
[NMR paper] Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase The role of biomolecular condensates in regulating biological function and the importance of dynamic interactions involving intrinsically disordered protein regions (IDRs) in their assembly are increasingly appreciated. While computational and theoretical approaches have provided significant insights into IDR phase behavior, establishing the critical interactions that govern condensation with atomic resolution through experiment is more difficult, given the lack of applicability of standard... ...	nmrlearner	Journal club	0	06-02-2021 05:10 PM
Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase [Biophysics and Computational Biology] Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase Tae Hun Kim, Brandon J. Payliss, Michael L. Nosella, Ian T. W. Lee, Yuki Toyama, Julie D. Forman-Kay, Lewis E. Kay... Date: 2021-06-01 The role of biomolecular condensates in regulating biological function and the importance of dynamic interactions involving intrinsically disordered protein regions (IDRs) in their assembly are increasingly appreciated. While computational and theoretical approaches have provided significant insights into IDR phase behavior, establishing the critical...	nmrlearner	Journal club	0	06-01-2021 11:42 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off