[NMR paper] Tandem NMR and mass spectrometry analysis of human nuclear membrane lipids.

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Tandem NMR and mass spectrometry analysis of human nuclear membrane lipids.

Tandem NMR and mass spectrometry analysis of human nuclear membrane lipids.

Anal Chem. 2020 Apr 23;:

Authors: Dazzoni R, Buré C, Morvan E, Grélard A, Gounou C, Schmitter JM, Loquet A, Larijani B, Dufourc EJ

Abstract
The human nuclear membrane is composed of a double bilayer; the inner membrane being linked to the protein lamina net-work and the outer nuclear membrane continuous with the endoplasmic reticulum. Nuclear membranes can form large invag-inations inside the nucleus whose role are poorly known. Although most of the protein identification has been determined, their lipid composition remains largely undetermined. In order to understand the mechanical and dynamic properties of nu-clear membranes we investigated their lipid composition by two quantitative methods, 31P and 1H multidimensional NMR and mass spectrometry using internal standards. We also developed a non-detergent nuclei extraction protocol allowing to produce milligram quantities of nuclear membrane lipids. We found that the nuclear membrane lipid extract is composed of a complex mixture of phospholipids with phosphatidylcholines present in large amounts. Negatively charged lipids, with high amounts of phosphoinositides were also present. Mass spectrometry confirmed the phospholipid composition and provided further information on acyl-chain length and unsaturation. Lipid chain lengths range between 30 to 38 carbon atoms (two chains summed up) with a high proportion of 34 carbon atom length for most species. PI lipids have large amounts of chain length with 36-38 carbons. Independent of the chain length unsaturations were highly elevated with 1 to 2 double bonds per lipid species.


PMID: 32324380 [PubMed - as supplied by publisher]



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