Related ArticlesSynthesis of Isotopically Labelled All-Trans Retinals for DNP-Enhanced Solid State NMR Studies of Retinylidene Proteins.
J Labelled Comp Radiopharm. 2017 Oct 28;:
Authors: Leeder AJ, Brown LJ, Becker-Baldus J, Mehler M, Glaubitz C, Brown RCD
Abstract
Three all-trans retinals containing multiple (13) C labels have been synthesised to enable DNP enhanced solid-state MAS NMR studies of novel microbial retinylidene membrane proteins including proteorhodpsin and channelrhodopsin. The synthetic aproaches allowed specific introduction of (13) C labels in ring substituents and at different positions in the polyene chain to probe structural features such as ring orientation and interaction of the chromophore with the protein in the ground state and in photointermediates. [10-18-(13) C9 ]-All-trans-retinal (1b), [12,15-(13) C2 ]-all-trans-retinal (1c) and [14,15-(13) C2 ]-all-trans-retinal (1d) were synthesised in in twelve, eight and seven linear steps from ethyl 2-oxocyclohexanecarboxylate (5) or ?-ionone (4) respectively.
PMID: 29080288 [PubMed - as supplied by publisher]
DNP-Enhanced Ultrawideline Solid-State NMR Spectroscopy: Studies of Platinum in Metal-Organic Frameworks #DNPNMR
From The DNP-NMR Blog:
DNP-Enhanced Ultrawideline Solid-State NMR Spectroscopy: Studies of Platinum in Metal-Organic Frameworks #DNPNMR
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Kobayashi, T., et al., DNP-Enhanced Ultrawideline Solid-State NMR Spectroscopy: Studies of Platinum in Metal-Organic Frameworks. J Phys Chem Lett, 2016. 7(13): p. 2322-7.
https://www.ncbi.nlm.nih.gov/pubmed/27266444
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01-12-2017 03:48 AM
Solid state NMR of isotope labelled murine fur: a powerful tool to study atomic level keratin structure and treatment effects
Solid state NMR of isotope labelled murine fur: a powerful tool to study atomic level keratin structure and treatment effects
Abstract
We have prepared mouse fur extensively 13C,15N-labelled in all amino acid types enabling application of 2D solid state NMR techniques which establish covalent and spatial proximities within, and in favorable cases between, residues. 13C double quantumâ??single quantum correlation and proton driven spin diffusion techniques are particularly useful for resolving certain amino acid types. Unlike 1D experiments on...
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11-19-2016 08:35 PM
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Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-oup_oa.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural...
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J Biomol NMR. 2013 Dec 13;
Authors: Ward ME, Wang S, Krishnamurthy S, Hutchins H, Fey M, Brown LS, Ladizhansky V
Abstract
Magic angle spinning nuclear magnetic resonance (MAS NMR) is well suited for the study of membrane proteins in membrane mimetic and native membrane...
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Biochemistry. 2003 Apr 1;42(12):3527-35
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In the HIV-1 gp41 and other viral fusion proteins, the minimal oligomerization state is believed to be trimeric with three N-terminal fusion peptides inserting into the membrane in close proximity. Previous studies have demonstrated that the...
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Production of recombinant isotopically labelled peptide by fusion to an insoluble par
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Mol Biosyst. 2010 Oct 18;
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The integrin ?v?6 is up-regulated in several cancers and has clinical potential for both tumour imaging and therapy. Peptide ligands have been developed...
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http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs.
Biochimie. 1997 Jul;79(7):415-22
Authors: Pavlov MY, Freistroffer DV, Ehrenberg M
A method to synthesise region-labelled proteins for structural studies with NMR is suggested. The technique is based on in vitro...
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08-22-2010 03:31 PM
[NMR paper] Synthesis of region-labelled proteins for NMR studies by in vitro translation of colu
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http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs.
Biochimie. 1997 Jul;79(7):415-22
Authors: Pavlov MY, Freistroffer DV, Ehrenberg M
A method to synthesise region-labelled proteins for structural studies with NMR is suggested. The technique is based on in vitro...
Synthesis of Isotopically Labelled All-Trans Retinals for DNP-Enhanced Solid State NMR Studies of Retinylidene Proteins.
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