NMR paper Synthesis and interaction studies of 13C labeled lactone derivatives with a model pro

		BioNMR > Educational resources > Journal club
[NMR paper] Synthesis and interaction studies of 13C labeled lactone derivatives with a model pro

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 03:01 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Synthesis and interaction studies of 13C labeled lactone derivatives with a model pro

Synthesis and interaction studies of 13C labeled lactone derivatives with a model protein using 13C NMR.

Related Articles Synthesis and interaction studies of 13C labeled lactone derivatives with a model protein using 13C NMR.

Bioorg Med Chem. 1993 Nov;1(5):389-97

Authors: Franot C, Benezra C, Lepoittevin JP

Two molecules 9 and 14, representatives of two series of electrophilic lactone derivatives, have been synthesized, and labeled with carbon 13 at their reactive sites. The mechanism and the products of the reaction of these two molecules with human serum albumin (HSA) under various reaction conditions have been studied by 13C NMR using DEPT 135 sequences. Results using the protein dissolved in aqueous medium or butylamine (a model nucleophile) dissolved in organic solvent were very similar. These results are entirely consistent with the in vivo allergising activity of these molecules. The validity of the Relative Alkylation Index (RAI) as a predicative model in contact allergy is discussed.

PMID: 8081868 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Cell-free synthesis of 15N-labeled proteins for NMR studies. Cell-free synthesis of 15N-labeled proteins for NMR studies. Related Articles Cell-free synthesis of 15N-labeled proteins for NMR studies. IUBMB Life. 2005 Sep;57(9):615-22 Authors: Ozawa K, Dixon NE, Otting G Modern cell-free in vitro protein synthesis systems present powerful tools for the synthesis of isotope-labeled proteins in high yields. The production of selectively 15 N-labeled proteins from 15 N-labeled amino acids is particularly economic and yields are often sufficient to analyze the proteins very quickly by two-dimensional NMR...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Interaction of the eukaryotic pore-forming cytolysin equinatoxin II with model membra Interaction of the eukaryotic pore-forming cytolysin equinatoxin II with model membranes: 19F NMR studies. Related Articles Interaction of the eukaryotic pore-forming cytolysin equinatoxin II with model membranes: 19F NMR studies. J Mol Biol. 2005 Mar 18;347(1):27-39 Authors: Anderluh G, Razpotnik A, Podlesek Z, Macek P, Separovic F, Norton RS Sea anemones produce a family of 18-20 kDa proteins, the actinoporins, which lyse cells by forming pores in cell membranes. Sphingomyelin plays an important role in their lytic activity, with membranes...	nmrlearner	Journal club	0	11-24-2010 11:14 PM
[NMR paper] A simple efficient synthesis of [23,24]-(13)C(2)-labeled bile salts as NMR probes of A simple efficient synthesis of -(13)C(2)-labeled bile salts as NMR probes of protein-ligand interactions. Related Articles A simple efficient synthesis of -(13)C(2)-labeled bile salts as NMR probes of protein-ligand interactions. Bioorg Med Chem Lett. 2002 Feb 11;12(3):433-5 Authors: Tochtrop GP, DeKoster GT, Cistola DP, Covey DF The synthesis of -(13)C(2)-labeled bile salts is achieved through a steroidal side chain degradation and isotopic regeneration strategy. Three common bile acids were degraded to the corresponding C(22 )aldehyde by an...	nmrlearner	Journal club	0	11-24-2010 08:49 PM
[NMR paper] 1H NMR studies on the interaction of beta-carboline derivatives with human serum albu 1H NMR studies on the interaction of beta-carboline derivatives with human serum albumin. Related Articles 1H NMR studies on the interaction of beta-carboline derivatives with human serum albumin. J Magn Reson. 1998 Feb;130(2):281-6 Authors: Veglia G, Delfini M, Giudice MR, Gaggelli E, Valensin G 1H NMR studies were performed on two beta-carboline derivatives interacting with human serum albumin. The spin-lattice relaxation rates of the two derivatives, having side chains of different length and polarity, were used to demonstrate a diverse...	nmrlearner	Journal club	0	11-17-2010 11:06 PM
[NMR paper] Efficient enzymatic synthesis of 13C,15N-labeled DNA for NMR studies. Efficient enzymatic synthesis of 13C,15N-labeled DNA for NMR studies. Related Articles Efficient enzymatic synthesis of 13C,15N-labeled DNA for NMR studies. J Biomol NMR. 1997 Oct;10(3):245-53 Authors: Smith DE, Su JY, Jucker FM The power of heteronuclear NMR spectroscopy to study macromolecules and their complexes has been amply demonstrated over the last decade. The obstacle to routinely applying these techniques to the study of DNA has been the synthesis of 13C,15N-labeled DNA. Here we present a simple and efficient method to generate...	nmrlearner	Journal club	0	08-22-2010 05:08 PM
[NMR paper] Model of interaction between a cardiotoxin and dimyristoylphosphatidic acid bilayers Model of interaction between a cardiotoxin and dimyristoylphosphatidic acid bilayers determined by solid-state 31P NMR spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Model of interaction between a cardiotoxin and dimyristoylphosphatidic acid bilayers determined by solid-state 31P NMR spectroscopy. Biophys J. 1996 Apr;70(4):1737-44 Authors: Picard F,...	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[NMR paper] Locations of local anesthetic dibucaine in model membranes and the interaction betwee Locations of local anesthetic dibucaine in model membranes and the interaction between dibucaine and a Na+ channel inactivation gate peptide as studied by 2H- and 1H-NMR spectroscopies. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Locations of local anesthetic dibucaine in model membranes and the interaction between dibucaine and a Na+ channel inactivation gate peptide as studied by 2H-...	nmrlearner	Journal club	0	08-22-2010 02:20 PM
[NMR paper] Synthesis of isotope labeled oligonucleotides and their use in an NMR study of a prot Synthesis of isotope labeled oligonucleotides and their use in an NMR study of a protein-DNA complex. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Synthesis of isotope labeled oligonucleotides and their use in an NMR study of a protein-DNA complex. Nucleic Acids Res. 1992 Feb 25;20(4):653-7 Authors: Kellenbach ER, Remerowski ML, Eib D, Boelens R, van der Marel GA, van den Elst H, van Boom JH, Kaptein R The synthesis of an oligonucleotide labeled with 13C...	nmrlearner	Journal club	0	08-21-2010 11:41 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off