NMR paper Synthesis of 5-methylaminomethyl-2-selenouridine in tRNAs: 31P NMR studies show the l

		BioNMR > Educational resources > Journal club
[NMR paper] Synthesis of 5-methylaminomethyl-2-selenouridine in tRNAs: 31P NMR studies show the l

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 11:41 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Synthesis of 5-methylaminomethyl-2-selenouridine in tRNAs: 31P NMR studies show the l

Synthesis of 5-methylaminomethyl-2-selenouridine in tRNAs: 31P NMR studies show the labile selenium donor synthesized by the selD gene product contains selenium bonded to phosphorus.

Related Articles Synthesis of 5-methylaminomethyl-2-selenouridine in tRNAs: 31P NMR studies show the labile selenium donor synthesized by the selD gene product contains selenium bonded to phosphorus.

Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2975-9

Authors: Veres Z, Tsai L, Scholz TD, Politino M, Balaban RS, Stadtman TC

An enzyme preparation from Salmonella typhimurium catalyzes the conversion of 5-methylaminomethyl-2-thiouridine in tRNAs to 5-methylaminomethyl-2-selenouridine when supplemented with selenide and ATP. Similar preparations from a Salmonella mutant strain carrying a defective selD gene fail to catalyze this selenium substitution reaction. However, supplementation of the deficient enzyme preparation with the purified selD gene product (SELD protein) restored synthesis of seleno-tRNAs. In the absence of the complementary enzyme(s), the SELD protein catalyzes the synthesis of a labile selenium donor compound from selenide and ATP. 31P NMR studies show that among the products of this reaction are AMP and a compound containing selenium bonded to phosphorus. The reaction is completely dependent on the addition of both selenide and magnesium. The dependence of reaction velocity on ATP concentration shows sigmoidal kinetics, whereas dependence on selenide concentration obeys Michaelis-Menten kinetics indicating a Km value of 46 microM for selenide.

PMID: 1557403 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Cell-free synthesis of 15N-labeled proteins for NMR studies. Cell-free synthesis of 15N-labeled proteins for NMR studies. Related Articles Cell-free synthesis of 15N-labeled proteins for NMR studies. IUBMB Life. 2005 Sep;57(9):615-22 Authors: Ozawa K, Dixon NE, Otting G Modern cell-free in vitro protein synthesis systems present powerful tools for the synthesis of isotope-labeled proteins in high yields. The production of selectively 15 N-labeled proteins from 15 N-labeled amino acids is particularly economic and yields are often sufficient to analyze the proteins very quickly by two-dimensional NMR...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Fatty acid synthesis in Xylella fastidiosa: correlations between genome studies, 13C Fatty acid synthesis in Xylella fastidiosa: correlations between genome studies, 13C NMR data, and molecular models. Related Articles Fatty acid synthesis in Xylella fastidiosa: correlations between genome studies, 13C NMR data, and molecular models. Biochem Biophys Res Commun. 2004 Oct 22;323(3):987-95 Authors: Osiro D, Muniz JR, Coleta Filho HD, de Sousa AA, Machado MA, Garratt RC, Colnago LA Xylella fastidiosa was the first plant pathogen to have its complete genome sequence elucidated. Routine database analyses suggested that two enzymes...	nmrlearner	Journal club	0	11-24-2010 10:01 PM
[NMR paper] NMR and modeling studies of protein-carbohydrate interactions: synthesis, three-dimen NMR and modeling studies of protein-carbohydrate interactions: synthesis, three-dimensional structure, and recognition properties of a minimum hevein domain with binding affinity for chitooligosaccharides. Related Articles NMR and modeling studies of protein-carbohydrate interactions: synthesis, three-dimensional structure, and recognition properties of a minimum hevein domain with binding affinity for chitooligosaccharides. Chembiochem. 2004 Sep 6;5(9):1245-55 Authors: Aboitiz N, Vila-Perelló M, Groves P, Asensio JL, Andreu D, Cañada FJ, Jiménez-Barbero...	nmrlearner	Journal club	0	11-24-2010 10:01 PM
[NMR paper] 15N NMR relaxation studies of calcium-loaded parvalbumin show tight dynamics compared 15N NMR relaxation studies of calcium-loaded parvalbumin show tight dynamics compared to those of other EF-hand proteins. Related Articles 15N NMR relaxation studies of calcium-loaded parvalbumin show tight dynamics compared to those of other EF-hand proteins. Biochemistry. 1998 Jul 14;37(28):9964-75 Authors: Baldellon C, Alattia JR, Strub MP, Pauls T, Berchtold MW, Cavé A, Padilla A Dynamics of the rat alpha-parvalbumin calcium-loaded form have been determined by measurement of 15N nuclear relaxation using proton-detected heteronuclear NMR...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] Efficient enzymatic synthesis of 13C,15N-labeled DNA for NMR studies. Efficient enzymatic synthesis of 13C,15N-labeled DNA for NMR studies. Related Articles Efficient enzymatic synthesis of 13C,15N-labeled DNA for NMR studies. J Biomol NMR. 1997 Oct;10(3):245-53 Authors: Smith DE, Su JY, Jucker FM The power of heteronuclear NMR spectroscopy to study macromolecules and their complexes has been amply demonstrated over the last decade. The obstacle to routinely applying these techniques to the study of DNA has been the synthesis of 13C,15N-labeled DNA. Here we present a simple and efficient method to generate...	nmrlearner	Journal club	0	08-22-2010 05:08 PM
[NMR paper] Synthesis of region-labelled proteins for NMR studies by in vitro translation of colu Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs. Biochimie. 1997 Jul;79(7):415-22 Authors: Pavlov MY, Freistroffer DV, Ehrenberg M A method to synthesise region-labelled proteins for structural studies with NMR is suggested. The technique is based on in vitro...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] Synthesis and interaction studies of 13C labeled lactone derivatives with a model pro Synthesis and interaction studies of 13C labeled lactone derivatives with a model protein using 13C NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Synthesis and interaction studies of 13C labeled lactone derivatives with a model protein using 13C NMR. Bioorg Med Chem. 1993 Nov;1(5):389-97 Authors: Franot C, Benezra C, Lepoittevin JP Two molecules 9 and 14, representatives of two series of electrophilic lactone derivatives, have been synthesized, and labeled with...	nmrlearner	Journal club	0	08-22-2010 03:01 AM
Cell-free protein synthesis of perdeuterated proteins for NMR studies Cell-free protein synthesis of perdeuterated proteins for NMR studies Touraj Etezady-Esfarjani, Sebastian Hiller, Cristina Villalba and Kurt Wüthrich Journal of Biomolecular NMR; 2007; 39(3); pp 229-238 Abstract: Cell-free protein synthesis protocols for uniformly deuterated proteins typically yield low, non-uniform deuteration levels. This paper introduces an E. coli cell-extract, D-S30, which enables efficient production of proteins with high deuteration levels for all non-labile hydrogen atom positions. Potential applications of the new protocol may include production of proteins...	Deano	Journal club	0	08-14-2008 10:01 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off