Publication year: 2011 Source: Journal of Magnetic Resonance, Available online 20 October 2011
Matthias*Huber, Oliver*With, Paul*Schanda, René*Verel, Matthias*Ernst, ...
Partial deuteration is a powerful tool to increase coherence life times and spectral resolution in proton solid-state NMR. The J coupling to deuterium needs, however, to be decoupled to maintain the good resolution in the (usually indirect)C dimension(s). We present a simple and reversible way to expand a commercial 1.3mm HCN MAS probe with aH channel with sufficient field strength for scalar deuterium decoupling, namely 2-3 kHz. The coil is placed at the outside of the stator and requires no significant modifications to the probe. The performance and the realizable gains in sensitivity and resolution are demonstrated using perdeuterated ubiquitin, with selectively CHD2-labeled methyl groups. Graphical abstract
Highlights
? Deuterium decoupling leads to enhanced resolution in perdeuterated proteins. ? We describe a reversible modification for a commercial probehead for 2H decoupling. ? Peak intensity increases by a factor of 2.4 in ubiquitin.
[Question from NMRWiki Q&A forum] adiabatic decoupling
adiabatic decoupling
Hi NMRwikiers, for measuring scalar couplings we use some HSQC pulse sequences modified for obtaining the coupling in F1-dimension as a splitting of doublets for CH2 groups. As high resolution is needed we use high folding in the acqusition, but as a result we obtain a lot of coupling artifacts in phenyl signals. We think that the use of adiabatic decoupling schemes should give a reasonably good spectrum of phenyls but I have no experience in adiabatic decoupling. Anyone can give me a hand for Bruker spectrometers?
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nmrlearner
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05-24-2011 10:02 PM
[Stan NMR blog] Commercial fMRI lie detection service
Commercial fMRI lie detection service
'No Lie MRI' - the very first commercial venture offering fMRI lie detection services
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01-12-2011 01:32 AM
Decoupling, what modultaion frequency to use ?
Hi all,
Perhaps a stupid question, but so far no one answered this one to me.
I want to find out what decoupler modulation frequency (dmf in Varian syntax) is needed to properly decouple observed couplings. Can anyone tell me ? I am looking for a practical answer.
I found a paper on decoupling, i.e. Shaka, A.J., Keeler, J. (1987): Progr. NMR Spectroscopy, 19, 47-129. Although this paper is formally correct, it is also very hard to read.
So let's make a practical example:
On an 800MHz machine, the carrier is on water (4.5 ppm), and there are imino protons resonating at 12-14 ppm....
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12-13-2010 06:56 AM
[Stan NMR blog] Commercial fMRI lie detection service
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08-27-2010 06:16 PM
[NMR paper] Removal of zero-quantum coherence in protein NMR spectra using SESAM decoupling and s
Removal of zero-quantum coherence in protein NMR spectra using SESAM decoupling and suppression of decoupling sidebands.
Related Articles Removal of zero-quantum coherence in protein NMR spectra using SESAM decoupling and suppression of decoupling sidebands.
J Magn Reson B. 1996 Feb;110(2):219-24
Authors: Weigelt J, Hammarstroem A, Bermel W, Otting G
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08-22-2010 02:27 PM
[U. of Ottawa NMR Facility Blog] Deuterium Decoupling
Deuterium Decoupling
Bruker NMR spectrometers equipped with a 2H TX board and 2H amplifier can be setup to carry out 2H observation and decoupling experiments while still maintaining a 2H lock. Two channel systems can use the 2H transmitter for 2H, 1H, 2H and X measurements. In addition to these, a three channel system can be set up for X measurements. The first figure below shows the 13C and 13C spectra collected for a mixture of deuterated solvents using two channels of an AVANCE spectrometer. The second figure shows the 13C, 13C, 13C and 13C spectra for the standard 13C sensitivity...
nmrlearner
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08-21-2010 08:15 PM
NMR Commercial Companies
Commercial Companies Norell, Inc - is dedicated to the advancement of NMR Spectroscopy by producing world-class NMR sample tubes. We conduct research and development, use state-of-the-art equipment and review our methods of operation in a never- ending effort to continually improve the quality of the products and services we offer.
Gilson 215 Liquid Handler
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GMW Associates
A supplier of magnet power supplies. These...
ale51
BioNMR Wiki
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12-15-2009 09:57 AM
2H decoupling in gNhsqc
hi, I had an insteresting problem with 2H decoupling on the Varian 600 machine for the gNhsqc experiment. I am doing locking and shimming without 2H decoupling. once you set dn3='H2' and submit the experiment, the lock level with drop to around half. and for the similar ghnca_P expriment, there is no such problem. the lock always stays the same level no matter decoupling on or off. Could anyone give me some suggestion? thanks.
A supplementary coil forH decoupling with commercial HCN MAS probes
Linear Mode
