Spectral resolution remains one of the most significant limitations in the NMR study of biomolecules. We present the srNOESY (super resolution nuclear overhauser effect spectroscopy) experiment, which enhances the resolution of NOESY cross-peaks at the expense of the diagonal peak line-width. We studied two proteins, ubiquitin and the influenza hemagglutinin fusion peptide in bicelles, and we achieved average resolution enhancements of 21â??47% and individual peak enhancements as large as ca. 450%. New peaks were observed over the conventional NOESY experiment in both proteins as a result of these improvements, and the final structures generated from the calculated restraints matched published models. We discuss the impact of the experimental parameters, spin diffusion and the information content of the srNOESY lineshape.
[NMR paper] Evaluation of the reliability of the maximum entropy method for reconstructing 3D and 4D NOESY-type NMR spectra of proteins.
Evaluation of the reliability of the maximum entropy method for reconstructing 3D and 4D NOESY-type NMR spectra of proteins.
Evaluation of the reliability of the maximum entropy method for reconstructing 3D and 4D NOESY-type NMR spectra of proteins.
Biochem Biophys Res Commun. 2014 Dec 26;
Authors: Shigemitsu Y, Ikeya T, Yamamoto A, Tsuchie Y, Mishima M, Smith BO, Güntert P, Ito Y
Abstract
Despite their advantages in analysis, 4D NMR experiments are still infrequently used as a routine tool in protein NMR projects due to the...
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12-30-2014 03:28 PM
Evaluation of the reliability of the maximum entropy method for reconstructing 3D and 4D NOESY-type NMR spectra of proteins
Evaluation of the reliability of the maximum entropy method for reconstructing 3D and 4D NOESY-type NMR spectra of proteins
Publication date: Available online 26 December 2014
Source:Biochemical and Biophysical Research Communications</br>
Author(s): Yoshiki Shigemitsu , Teppei Ikeya , Akihiro Yamamoto , Yuusuke Tsuchie , Masaki Mishima , Brian O. Smith , Peter Güntert , Yutaka Ito</br>
Despite their advantages in analysis, 4D NMR experiments are still infrequently used as a routine tool in protein NMR projects due to the long duration of the measurement and...
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12-27-2014 03:04 AM
A 3D time-shared NOESY experiment designed to provide optimal resolution for accurate assignment of NMR distance restraints in large proteins
A 3D time-shared NOESY experiment designed to provide optimal resolution for accurate assignment of NMR distance restraints in large proteins
Abstract
Structure determination of proteins by solution NMR has become an established method, but challenges increase steeply with the size of proteins. Notably, spectral crowding and signal overlap impair the analysis of cross-peaks in NOESY spectra that provide distance restraints for structural models. An optimal spectral resolution can alleviate overlap but requires prohibitively long experimental time...
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11-11-2014 11:57 AM
[NMR paper] A 3D time-shared NOESY experiment designed to provide optimal resolution for accurate assignment of NMR distance restraints in large proteins.
A 3D time-shared NOESY experiment designed to provide optimal resolution for accurate assignment of NMR distance restraints in large proteins.
Related Articles A 3D time-shared NOESY experiment designed to provide optimal resolution for accurate assignment of NMR distance restraints in large proteins.
J Biomol NMR. 2014 Nov 9;
Authors: Mishra SH, Harden BJ, Frueh DP
Abstract
Structure determination of proteins by solution NMR has become an established method, but challenges increase steeply with the size of proteins. Notably,...
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11-10-2014 10:59 PM
[NMR paper] Modeling Proteins Using a Super-Secondary Structure Library and NMR Chemical Shift Information.
Modeling Proteins Using a Super-Secondary Structure Library and NMR Chemical Shift Information.
Related Articles Modeling Proteins Using a Super-Secondary Structure Library and NMR Chemical Shift Information.
Structure. 2013 May 14;
Authors: Menon V, Vallat BK, Dybas JM, Fiser A
Abstract
A remaining challenge in protein modeling is to predict structures for sequences with no sequence similarity to any experimentally solved structure. Based on earlier observations, the library of protein backbone supersecondary structure motifs (Smotifs)...
Compressed sensing reconstruction of undersampled 3D NOESY spectra: application to large membrane proteins
Compressed sensing reconstruction of undersampled 3D NOESY spectra: application to large membrane proteins
Abstract Central to structural studies of biomolecules are multidimensional experiments. These are lengthy to record due to the requirement to sample the full Nyquist grid. Time savings can be achieved through undersampling the indirectly-detected dimensions combined with non-Fourier Transform (FT) processing, provided the experimental signal-to-noise ratio is sufficient. Alternatively, resolution and signal-to-noise can be improved within a given experiment time. However, non-FT...
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07-30-2012 07:42 AM
Efficient Acquisition of High-Resolution 4-D Diagonal-Suppressed Methyl-Methyl NOESY for Large Proteins
Efficient Acquisition of High-Resolution 4-D Diagonal-Suppressed Methyl-Methyl NOESY for Large Proteins
Publication year: 2012
Source:Journal of Magnetic Resonance</br>
Jie Wen, Jihui Wu, Pei Zhou</br>
The methyl-methyl NOESYexperimentplays an important role in determiningthe global folds of large proteins. Despite the high sensitivity of this experiment, the analysisof methyl-methyl NOEs is frequently hindered by the limited chemical shift dispersion of methyl groups, particularly methyl protons. Thismakes it difficult to unambiguously assign all of the methyl-methyl...
Super resolution NOESY spectra of proteins
Linear Mode
