The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins for insect preys. In this respect, spiders as a highly diversified group of almost exclusive insect predators appear to possess infinite potential for the discovery of novel insect-selective toxins. In 2003, a group of toxins was isolated from the spider Macrothele gigas and the amino acid sequence was reported. We obtained, by molecular biology techniques in a heterologous system, one of these toxins. Purification process was optimized by chromatographic methods to determine the three-dimensional structure by nuclear magnetic resonance in solution, and, finally, their biological activity was tested. rMagi3 resulted to be a specific insect toxin with no effect on mice.
Successful refolding and NMR structure of rMagi3: a disulfide-rich insecticidal spider toxin
Successful refolding and NMR structure of rMagi3: a disulfide-rich insecticidal spider toxin
Abstract
The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins for insect preys. In this respect, spiders as a highly diversified group of almost exclusive insect predators appear to possess infinite potential for the discovery of novel insect-selective toxins. In 2003, a group of toxins was isolated from the spider Macrothele gigas and the amino acid sequence was reported. We obtained, by molecular...
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01-04-2018 08:45 AM
[NMR paper] Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin.
Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin.
Related Articles Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin.
Protein Sci. 2017 Dec 16;:
Authors: Titaux-Delgado G, Carrillo E, Mendoza A, Mayorga-Flores M, Escobedo-González FC, Cano-Sánchez P, López-Vera E, Corzo G, Del Rio-Portilla F
Abstract
The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly...
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12-19-2017 11:31 AM
Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin
Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin
Abstract
The need for molecules with high specificity against noxious insects leads the search towards spider venoms that have evolved highly selective toxins for insect preys. In this respect, spiders as a highly diversified group of almost exclusive insect predators appear to possess infinite potential for the discovery of novel insect-selective toxins. In 2003 a group of toxins was isolated from the spider Macrothele gigas and the amino acid sequence was reported. We obtained, by molecular...
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12-17-2017 10:34 AM
[NMR paper] Probing pattern and dynamics of disulfide bridges using synthesis and NMR of an ion channel blocker peptide toxin with multiple diselenide bonds.
Probing pattern and dynamics of disulfide bridges using synthesis and NMR of an ion channel blocker peptide toxin with multiple diselenide bonds.
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Chem Sci. 2016 Apr 21;7(4):2666-2673
Authors: Fehér K, Timári I, Rákosi K, Szolomájer J, Illyés TZ, Bartok A, Varga Z, Panyi G, Tóth GK, Kövér KE
Abstract
Anuroctoxin (AnTx), a 35-amino-acid scorpion toxin containing four...
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07-03-2017 02:31 AM
[NMR paper] NMR structure and function of Helicoverpa armigera sterol carrier protein-2, an important insecticidal target from the cotton bollworm.
NMR structure and function of Helicoverpa armigera sterol carrier protein-2, an important insecticidal target from the cotton bollworm.
NMR structure and function of Helicoverpa armigera sterol carrier protein-2, an important insecticidal target from the cotton bollworm.
Sci Rep. 2015;5:18186
Authors: Ma H, Ma Y, Liu X, Dyer DH, Xu P, Liu K, Lan Q, Hong H, Peng J, Peng R
Abstract
The cotton bollworm, Helicoverpa armigera, has developed strong resistance to many insecticides. Sterol Carrier Protein-2 (SCP-2) is an important...
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12-15-2015 08:09 PM
[NMR paper] Three-dimensional structure of the RGD-containing snake toxin albolabrin in solution,
Three-dimensional structure of the RGD-containing snake toxin albolabrin in solution, based on 1H NMR spectroscopy and simulated annealing calculations.
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Int J Pept Protein Res. 1996 Sep;48(3):220-8
Authors: Smith KJ, Jaseja M, Lu X, Williams JA, Hyde EI, Trayer IP
Albolabrin is a snake toxin that contains a RGD-(Arg-Gly-Asp) sequence motif and competes with fibrinogen to bind...
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08-22-2010 02:20 PM
[NMR paper] Primary and NMR three-dimensional structure determination of a novel crustacean toxin
Primary and NMR three-dimensional structure determination of a novel crustacean toxin from the venom of the scorpion Centruroides limpidus limpidus Karsch.
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Biochemistry. 1994 Sep 20;33(37):11135-49
Authors: Lebreton F, Delepierre M, RamĂ*rez AN, Balderas C, Possani LD
A crustacean-specific toxin from the Mexican scorpion Centruroides limpidus limpidus was purified, and its...
Successful refolding and NMR structure of rMagi3: A disulfide-rich insecticidal spider toxin
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