NMR paper Sub-second 2D NMR spectroscopy at sub-millimolar concentrations.

		BioNMR > Educational resources > Journal club
[NMR paper] Sub-second 2D NMR spectroscopy at sub-millimolar concentrations.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 10:01 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,733

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Sub-second 2D NMR spectroscopy at sub-millimolar concentrations.

Sub-second 2D NMR spectroscopy at sub-millimolar concentrations.

Related Articles Sub-second 2D NMR spectroscopy at sub-millimolar concentrations.

J Am Chem Soc. 2004 Sep 29;126(38):11756-7

Authors: Shapira B, Morris E, Muszkat KA, Frydman L

A recently proposed protocol enables the acquisition of two-dimensional nuclear magnetic resonance (2D NMR) spectra within a single scan. A promising application opened up by this new data acquisition mode concerns its combination with active nuclear polarization methods, whereby spectroscopy is carried out on analytes whose spin magnetizations have been significantly enhanced over their Boltzmann thermal values. The present paper explores the potential of such combination, with the acquisition of peptide and protein 2D NMR 1H correlation spectra recorded after the samples had been subject to laser-driven chemically induced dynamic nuclear polarization (CIDNP). It is demonstrated that the speed and sensitivity enhancement afforded by these combined processes enables the acquisition of quality 2D NMR data sets within a fraction of a second, at analyte concentrations that are under 1 mM.

PMID: 15382886 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Question from NMRWiki Q&A forum] Which are better methods of water suppression at higher salt concentrations? Which are better methods of water suppression at higher salt concentrations? Hello, could anyone recommend better technique for water suppression for the samples having higher salt concentration? At what salt concentration is the method effective? Dr. Talluri mentioned in his post that water suppression using soft pulses (such as one using excitation sculpting) is not as friendly for high salt samples - what is the mechanism?	nmrlearner	News from other NMR forums	0	08-22-2010 02:30 AM
[NMR paper] 31P NMR studies of ATP concentrations and Pi-ATP exchange in the rat kidney in vivo: 31P NMR studies of ATP concentrations and Pi-ATP exchange in the rat kidney in vivo: effects of inhibiting and stimulating renal metabolism. Related Articles 31P NMR studies of ATP concentrations and Pi-ATP exchange in the rat kidney in vivo: effects of inhibiting and stimulating renal metabolism. Magn Reson Med. 1990 Jun;14(3):445-60 Authors: Shine N, Xuan A, Weiner MW Previous investigators found that cyanide (CN-) is a potent inhibitor of renal Na+ transport, while the uncoupling agent 2,4-dinitrophenol (DNP) and fructose (both which lower...	nmrlearner	Journal club	0	08-21-2010 10:48 PM
Lipoprotein size, concentrations associated with incident type 2 diabetes - Endocrine Lipoprotein size, concentrations associated with incident type 2 diabetes - Endocrine Today <img alt="" height="1" width="1" /> Lipoprotein size, concentrations associated with incident type 2 diabetes Endocrine Today Mora and colleagues used nuclear magnetic resonance spectroscopy on a small blood specimen to measure baseline lipoprotein particle size and concentration, ...	nmrlearner	Online News	0	08-15-2010 03:09 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off