NMR spectroscopy is emerging as a powerful tool in molecular biology and biotechnology; one aspect of which is the use of one- and two-dimensional NMR methodologies to investigate the interactions of proteins with DNA. The dynamic and structural information which NMR can provide, on the changes in conformation and molecular flexibility, complements X-ray crystallography data and enables mechanistic models of DNA-protein interactions to be formulated.
[NMR paper] Photo-CIDNP NMR methods for studying protein folding.
Photo-CIDNP NMR methods for studying protein folding.
Related Articles Photo-CIDNP NMR methods for studying protein folding.
Methods. 2004 Sep;34(1):75-87
Authors: Mok KH, Hore PJ
Chemically induced dynamic nuclear polarization (CIDNP) is a nuclear magnetic resonance phenomenon that can be used to probe the solvent-accessibility of tryptophan, tyrosine, and histidine residues in proteins by means of laser-induced photochemical reactions, resulting in significant enhancement of NMR signals. CIDNP offers good sensitivity as a surface probe of...
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11-24-2010 10:01 PM
[NMR paper] NMR structure of Desulfovibrio gigas rubredoxin: a model for studying protein stabili
NMR structure of Desulfovibrio gigas rubredoxin: a model for studying protein stabilization by compatible solutes.
Related Articles NMR structure of Desulfovibrio gigas rubredoxin: a model for studying protein stabilization by compatible solutes.
Extremophiles. 2001 Oct;5(5):303-11
Authors: Lamosa P, Brennan L, Vis H, Turner DL, Santos H
Rubredoxins are small, soluble proteins that display a wide variation in thermostability, despite having a high degree of sequence similarity They also vary in the extent to which they are stabilized by...
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11-19-2010 08:44 PM
Ph.D. student in solid-state NMR: Studying protein folding and assembly at atomic sca
Ph.D. student in solid-state NMR: Studying protein folding and assembly at atomic scale
Description of the Faculty / Research group
The Faculty of Science consists of six departments: Biology, Pharmaceutical Sciences, Information and Computing Sciences, Physics and Astronomy, Mathematics and Chemistry. The Faculty is home to 3500 students and nearly 2000 staff and is internationally renowned for the quality of its research.
The NMR Research Group is part of the Chemistry Department (Universiteit Utrecht fac. Scheikunde) and belongs to the Bijvoet Center for Biomolecular...
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09-11-2010 07:15 PM
The STINT-NMR Method for Studying In-cell Protein-Protein Interactions.
The STINT-NMR Method for Studying In-cell Protein-Protein Interactions.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles The STINT-NMR Method for Studying In-cell Protein-Protein Interactions.
Curr Protoc Protein Sci. 2010 Aug;Chapter 17:Unit17.11
Authors: Burz DS, Shekhtman A
This unit describes critical components and considerations required to study protein-protein structural interactions inside a living cell by using NMR...
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09-05-2010 05:53 AM
[NMR paper] Solid-state NMR approaches for studying membrane protein structure.
Solid-state NMR approaches for studying membrane protein structure.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--arjournals.annualreviews.org-images-AnnualReviews100x25.gif Related Articles Solid-state NMR approaches for studying membrane protein structure.
Annu Rev Biophys Biomol Struct. 1992;21:25-47
Authors: Smith SO, Peersen OB
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08-21-2010 11:41 PM
Application of the random coil index to studying protein flexibility
Application of the random coil index to studying protein flexibility
Mark V. Berjanskii and David S. Wishart
Journal of Biomolecular NMR; 2008; 40(1); pp 31-48
Abstract:
Protein flexibility lies at the heart of many protein–ligand binding events and enzymatic activities. However, the experimental measurement of protein motions is often difficult, tedious and error-prone. As a result, there is a considerable interest in developing simpler and faster ways of quantifying protein flexibility. Recently, we described a method, called Random Coil Index (RCI), which appears to be able to...
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08-14-2008 01:03 AM
Postdoctoral position to study Protein-Protein Interactions and their role in Mechanisms of Signal Transduction using protein solution NMR/x-ray crystallography
Spincore.com are advertising a postdoc NMR position. It sounds pretty interesting.
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Postdoctoral position to study Protein-Protein Interactions and their role in Mechanisms of Signal Transduction using protein solution NMR /x-ray crystallography
Case Medical School, Cleveland, Ohio, USA
How are signaling events transmitted from one protein to another? To answer this question we are looking to add a postdoctoral co-workers to our interdisciplinary team. Our interest is to understand protein-protein interactions, protein structure and dynamics in the context of cell signaling...