Related ArticlesStructures of the platelet calcium- and integrin-binding protein and the alphaIIb-integrin cytoplasmic domain suggest a mechanism for calcium-regulated recognition; homology modelling and NMR studies.
J Mol Recognit. 2000 Mar-Apr;13(2):83-92
Authors: Hwang PM, Vogel HJ
Calcium- and integrin-binding protein (CIB) binds to the 20-residue alphaIIb cytoplasmic domain of platelet alphaIIbbeta3 integrin. Amino acid sequence similarities with calmodulin (CaM) and calcineurin B (CnB) allowed the construction of homology-based models of calcium-saturated CIB as well as apo-CIB. In addition, the solution structure of the alphaIIb cytoplasmic domain in 45% aqueous trifluoroethanol was solved by conventional two-dimensional NMR methods. The models indicate that the N-terminal domain of CIB possesses a number of positively charged residues in its binding site that could interact with the acidic carboxy-terminal LEEDDEEGE sequence of alphaIIb. The C-terminal domain of CIB seems well-suited to bind the sequence WKVGFFKR, which forms a well-structured alpha helix; this is analogous to calmodulin and calcineurin B, which also bind alpha helices. Similarities between the C-terminal domains of CIB and calmodulin suggest that binding of CIB to the cytoplasmic domain of alphaIIb may be affected by fluctuations in the intracellular calcium concentration.
Exploring NMR ensembles of calcium binding proteins: perspectives to design inhibitors of protein-protein interactions.
Exploring NMR ensembles of calcium binding proteins: perspectives to design inhibitors of protein-protein interactions.
Exploring NMR ensembles of calcium binding proteins: perspectives to design inhibitors of protein-protein interactions.
BMC Struct Biol. 2011 May 12;11(1):24
Authors: Isvoran A, Badel A, Craescu CT, Miron S, Miteva MA
ABSTRACT: BACKGROUND: Disrupting protein-protein interactions by small organic molecules is nowadays a promising strategy employed to block protein targets involved in different pathologies. However, structural...
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05-17-2011 06:21 PM
Calcium binding environments probed by (43)Ca NMR spectroscopy.
Calcium binding environments probed by (43)Ca NMR spectroscopy.
Calcium binding environments probed by (43)Ca NMR spectroscopy.
Dalton Trans. 2010 Oct 7;39(37):8593-602
Authors: Bryce DL
Calcium is an important component of materials, metalloproteins, minerals, glasses, and small inorganic and organic complexes. However, NMR spectroscopy of the quadrupolar (43)Ca nuclide remains difficult primarily due to its low natural abundance and low resonance frequency. In this Perspective, experimental challenges and recent successes in the field are...
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12-16-2010 09:21 PM
NMR Structure and Calcium-Binding Properties of the Tellurite Resistance Protein TerD from Klebsiella pneumoniae.
NMR Structure and Calcium-Binding Properties of the Tellurite Resistance Protein TerD from Klebsiella pneumoniae.
NMR Structure and Calcium-Binding Properties of the Tellurite Resistance Protein TerD from Klebsiella pneumoniae.
J Mol Biol. 2010 Nov 25;
Authors: Pan YR, Lou YC, Seven AB, Rizo J, Chen C
The tellurium oxyanion, TeO(3)(2-), has been used in the treatment of infectious diseases caused by mycobacteria. However, many pathogenic bacteria show tellurite resistance. Several tellurite resistance genes have been identified, and these genes...
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11-30-2010 08:14 PM
[NMR paper] NMR solution structure of calerythrin, an EF-hand calcium-binding protein from Saccha
NMR solution structure of calerythrin, an EF-hand calcium-binding protein from Saccharopolyspora erythraea.
Related Articles NMR solution structure of calerythrin, an EF-hand calcium-binding protein from Saccharopolyspora erythraea.
Eur J Biochem. 2003 Jun;270(11):2505-12
Authors: Tossavainen H, Permi P, Annila A, Kilpeläinen I, Drakenberg T
The structure of calerythrin, a prokaryotic 20 kDa calcium-binding protein has been determined by solution NMR spectroscopy. Distance, dihedral angle, J coupling, secondary chemical shift, residual dipolar...
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11-24-2010 09:01 PM
[NMR paper] Shape and dynamics of a calcium-binding protein investigated by nitrogen-15 NMR relax
Shape and dynamics of a calcium-binding protein investigated by nitrogen-15 NMR relaxation.
Related Articles Shape and dynamics of a calcium-binding protein investigated by nitrogen-15 NMR relaxation.
Methods Mol Biol. 2002;173:285-300
Authors: Werner JM, Campbell ID, Downing AK
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11-24-2010 08:49 PM
[NMR paper] NMR derived solution structure of an EF-hand calcium-binding protein from Entamoeba H
NMR derived solution structure of an EF-hand calcium-binding protein from Entamoeba Histolytica.
Related Articles NMR derived solution structure of an EF-hand calcium-binding protein from Entamoeba Histolytica.
Biochemistry. 2001 Dec 4;40(48):14392-403
Authors: Atreya HS, Sahu SC, Bhattacharya A, Chary KV, Govil G
We present the three-dimensional (3D) solution structure of a calcium-binding protein from Entamoeba histolytica (EhCaBP), an etiologic agent of amoebiasis affecting millions worldwide. EhCaBP is a 14.7 kDa (134 residues) monomeric...
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11-19-2010 08:44 PM
[NMR paper] Heparin binding to platelet factor-4. An NMR and site-directed mutagenesis study: arg
Heparin binding to platelet factor-4. An NMR and site-directed mutagenesis study: arginine residues are crucial for binding.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Heparin binding to platelet factor-4. An NMR and site-directed mutagenesis study: arginine residues are crucial for binding.
Biochem J. 1995 Dec 1;312 ( Pt 2):357-65
Authors: Mayo KH, Ilyina E, Roongta V, Dundas M, Joseph J, Lai CK, Maione T, Daly TJ
Native platelet factor-4 (PF4) is an...
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08-22-2010 03:50 AM
[KPWU blog] [IDP] RTX calcium-binding motifs
RTX calcium-binding motifs
Title:RTX calcium-binding motifs are intrinsically disordered in the absence of calcium: Implication for protein secretion Authors: Alexandre Chenal, J. Iņaki Guijarro, Bertrand Raynal, Muriel Delepierre, and Daniel Ladant Journal: J. Biol. Chem, 10.1074/jbc.M807312200 (online publish first) Note: Not an NMR-based study.http://stats.wordpress.com/b.gif?host=kpwu.wordpress.com&blog=76132&post=194&subd=kpwu&ref=&feed=1
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Structures of the platelet calcium- and integrin-binding protein and the alphaIIb-int
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