Infectious proteins or prions are a remarkable class of pathogens, where pathogenicity and infectious state correspond to conformational transition of a protein fold. The conformational change translates into the formation by the protein of insoluble amyloid aggregates, associated in humans with various neurodegenerative disorders and systemic protein-deposition diseases. The prion principle, however, is not limited to pathogenicity. While pathological amyloids (and prions) emerge from protein...
[NMR paper] Robust Cell-Free Expression of Sub-Pathological and Pathological Huntingtin Exon-1 for NMR Studies. General Approaches for the Isotopic Labeling of Low-Complexity Proteins.
Robust Cell-Free Expression of Sub-Pathological and Pathological Huntingtin Exon-1 for NMR Studies. General Approaches for the Isotopic Labeling of Low-Complexity Proteins.
Robust Cell-Free Expression of Sub-Pathological and Pathological Huntingtin Exon-1 for NMR Studies. General Approaches for the Isotopic Labeling of Low-Complexity Proteins.
Biomolecules. 2020 Oct 19;10(10):
Authors: Morató A, Elena-Real CA, Popovic M, Fournet A, Zhang K, Allemand F, Sibille N, Urbanek A, Bernadó P
Abstract
The high-resolution structural...
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[NMR paper] Conformations and Intermolecular Interactions in Cellulose/Silk Fibroin Blend Films: A Solid-State NMR Perspective.
Conformations and Intermolecular Interactions in Cellulose/Silk Fibroin Blend Films: A Solid-State NMR Perspective.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Conformations and Intermolecular Interactions in Cellulose/Silk Fibroin Blend Films: A Solid-State NMR Perspective.
J Phys Chem B. 2017 06 29;121(25):6108-6116
Authors: Tian D, Li T, Zhang R, Wu Q, Chen T, Sun P, Ramamoorthy A
Abstract
Fabricating materials with excellent mechanical...
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[NMR paper] Membrane solid-state NMR in Canada: A historical perspective.
Membrane solid-state NMR in Canada: A historical perspective.
Related Articles Membrane solid-state NMR in Canada: A historical perspective.
Biochim Biophys Acta. 2017 Jun 23;:
Authors: Auger M
Abstract
This manuscript presents an overview of more than 40years of membrane solid-state nuclear magnetic resonance (NMR) research in Canada. This technique is a method of choice for the study of the structure and dynamics of lipid bilayers; bilayer interactions with a variety of molecules such as membrane peptides, membrane proteins and...
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[NMR paper] Multidimensional Solid-State NMR of a Functional Multiprotein Chemoreceptor Array.
Multidimensional Solid-State NMR of a Functional Multiprotein Chemoreceptor Array.
Related Articles Multidimensional Solid-State NMR of a Functional Multiprotein Chemoreceptor Array.
Biochemistry. 2016 Jun 13;
Authors: Harris MJ, Struppe JO, Wylie BJ, McDermott AE, Thompson LK
Abstract
The bacterial chemoreceptor complex governs signal detection and the upstream elements of chemotactic behavior, but the detailed molecular mechanism is still unclear. We have assembled native-like functional arrays of an aspartate receptor...
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Interview with Robert Tycko: On amyloids, Alzheimer disease, and solid-state NMR
From The DNP-NMR Blog:
Interview with Robert Tycko: On amyloids, Alzheimer disease, and solid-state NMR
Besides his contributions to solid-state NMR spectroscopy and it's application to study bio-macromolecular systems, Rob Tycko is also very active in DNP research.
Skrynnikov, N.R. and R. Tycko, Interview with Robert Tycko: On amyloids, Alzheimer disease, and solid-state NMR. Concepts in Magnetic Resonance Part A, 2015. 44(4): p. 182-189.
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[NMR paper] Proteins Dynamics in the Solid-state from (2)H NMR Lineshape Analysis: a Consistent Perspective.
Proteins Dynamics in the Solid-state from (2)H NMR Lineshape Analysis: a Consistent Perspective.
Related Articles Proteins Dynamics in the Solid-state from (2)H NMR Lineshape Analysis: a Consistent Perspective.
J Phys Chem B. 2015 Jan 16;
Authors: Meirovitch E, Liang Z, Freed JH
Abstract
Deuterium lineshape analysis of CD3 groups emerged as a particularly useful tool for studying ?s - ms protein motions in the solid-state. The models devised so far consist of several independently conceived simple jump-type motions. They are...
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[NMR paper] Automated solid-state NMR resonance assignment of protein microcrystals and amyloids.
Automated solid-state NMR resonance assignment of protein microcrystals and amyloids.
Related Articles Automated solid-state NMR resonance assignment of protein microcrystals and amyloids.
J Biomol NMR. 2013 May 21;
Authors: Schmidt E, Gath J, Habenstein B, Ravotti F, Székely K, Huber M, Buchner L, Böckmann A, Meier BH, Güntert P
Abstract
Solid-state NMR is an emerging structure determination technique for crystalline and non-crystalline protein assemblies, e.g., amyloids. Resonance assignment constitutes the first and often very...
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05-22-2013 04:43 PM
Heat management strategies for solid-state NMR of functional proteins
Heat management strategies for solid-state NMR of functional proteins
September 2012
Publication year: 2012
Source:Journal of Magnetic Resonance, Volume 222</br>
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Modern solid-state NMR methods can acquire high-resolution protein spectra for structure determination. However, these methods use rapid sample spinning and intense decoupling fields that can heat and denature the protein being studied. Here we present a strategy to avoid destroying valuable samples. We advocate first creating a sacrificial sample, which contains unlabeled protein (or no protein) in buffer...
Structures of Pathological and Functional Amyloids and Prions, a Solid-State NMR Perspective
Linear Mode
