Related ArticlesStructure of a malaria parasite antigenic determinant displayed on filamentous bacteriophage determined by NMR spectroscopy: implications for the structure of continuous peptide epitopes of proteins.
The NANP repeating sequence of the circumsporozoite protein of Plasmodium falciparum was displayed on the surface of fd filamentous bacteriophage as a 12-residue insert (NANP)(3) in the N-terminal region of the major coat protein (pVIII). The structure of the epitope determined by multidimensional solution NMR spectroscopy of the modified pVIII protein in lipid micelles was shown to be a twofold repeat of an extended and non-hydrogen-bonded loop based on the sequence NPNA, demonstrating that the repeating sequence is NPNA, not NANP. Further, high resolution solid-state NMR spectra of intact hybrid virions containing the modified pVIII proteins demonstrate that the peptides displayed on the surface of the virion adopt a single, stable conformation; this is consistent with their pronounced immunogenicity as well as their ability to mimic the antigenicity of their native parent proteins.
Consensus structure of Pf1 filamentous bacteriophage from X-ray fibre diffraction and solid-state NMR.
Consensus structure of Pf1 filamentous bacteriophage from X-ray fibre diffraction and solid-state NMR.
Consensus structure of Pf1 filamentous bacteriophage from X-ray fibre diffraction and solid-state NMR.
Eur Biophys J. 2011 Mar;40(3):221-34
Authors: Straus SK, Scott WR, Schwieters CD, Marvin DA
Filamentous bacteriophages (filamentous bacterial viruses or Inovirus) are simple and well-characterised macromolecular assemblies that are widely used in molecular biology and biophysics, both as paradigms for studying basic biological questions and as...
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06-15-2011 01:15 PM
[NMR paper] Precise epitope mapping of malaria parasite inhibitory antibodies by TROSY NMR cross-
Precise epitope mapping of malaria parasite inhibitory antibodies by TROSY NMR cross-saturation.
Related Articles Precise epitope mapping of malaria parasite inhibitory antibodies by TROSY NMR cross-saturation.
Biochemistry. 2005 Jan 18;44(2):518-23
Authors: Morgan WD, Frenkiel TA, Lock MJ, Grainger M, Holder AA
We have applied NMR cross-saturation with TROSY detection to the problem of precisely mapping conformational epitopes on complete protein antigen molecules. We have investigated complexes of the Fab fragments of two antibodies that...
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[NMR paper] Malaria parasite-inhibitory antibody epitopes on Plasmodium falciparum merozoite surf
Malaria parasite-inhibitory antibody epitopes on Plasmodium falciparum merozoite surface protein-1(19) mapped by TROSY NMR.
Related Articles Malaria parasite-inhibitory antibody epitopes on Plasmodium falciparum merozoite surface protein-1(19) mapped by TROSY NMR.
Mol Biochem Parasitol. 2004 Nov;138(1):29-36
Authors: Morgan WD, Lock MJ, Frenkiel TA, Grainger M, Holder AA
Plasmodium falciparum merozoite surface protein 1 (MSP1)(19), the C-terminal fragment of merozoite surface protein 1, is a leading candidate antigen for development of a...
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11-24-2010 10:03 PM
[NMR paper] Structure of the coat protein in fd filamentous bacteriophage particles determined by
Structure of the coat protein in fd filamentous bacteriophage particles determined by solid-state NMR spectroscopy.
Related Articles Structure of the coat protein in fd filamentous bacteriophage particles determined by solid-state NMR spectroscopy.
Proc Natl Acad Sci U S A. 2003 May 27;100(11):6458-63
Authors: Zeri AC, Mesleh MF, Nevzorov AA, Opella SJ
The atomic resolution structure of fd coat protein determined by solid-state NMR spectroscopy of magnetically aligned filamentous bacteriophage particles differs from that previously determined...
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11-24-2010 09:01 PM
[NMR paper] NMR structure of Plasmodium falciparum malaria peptide correlates with protective imm
NMR structure of Plasmodium falciparum malaria peptide correlates with protective immunity.
Related Articles NMR structure of Plasmodium falciparum malaria peptide correlates with protective immunity.
Biochim Biophys Acta. 2002 May 10;1571(1):27-33
Authors: Purmova J, Salazar LM, Espejo F, Torres MH, Cubillos M, Torres E, Lopez Y, Rodríguez R, Patarroyo ME
Apical membrane antigen-1 is an integral Plasmodium falciparum malaria parasite membrane protein. High activity binding peptides (HABPs) to human red blood cells (RBCs) have been identified...
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11-24-2010 08:49 PM
[NMR paper] NMR structure of the principal neutralizing determinant of HIV-1 displayed in filamen
NMR structure of the principal neutralizing determinant of HIV-1 displayed in filamentous bacteriophage coat protein.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR structure of the principal neutralizing determinant of HIV-1 displayed in filamentous bacteriophage coat protein.
J Mol Biol. 1997 Mar 7;266(4):649-55
Authors: Jelinek R, Terry TD, Gesell JJ, Malik P, Perham RN, Opella SJ
An NMR approach for structure determination of short peptides displayed on the...
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08-22-2010 03:31 PM
[NMR paper] NMR structure of the principal neutralizing determinant of HIV-1 displayed in filamen
NMR structure of the principal neutralizing determinant of HIV-1 displayed in filamentous bacteriophage coat protein.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR structure of the principal neutralizing determinant of HIV-1 displayed in filamentous bacteriophage coat protein.
J Mol Biol. 1997 Mar 7;266(4):649-55
Authors: Jelinek R, Terry TD, Gesell JJ, Malik P, Perham RN, Opella SJ
An NMR approach for structure determination of short peptides displayed on the...
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08-22-2010 03:03 PM
[NMR paper] Secondary structure of the single-stranded DNA binding protein encoded by filamentous
Secondary structure of the single-stranded DNA binding protein encoded by filamentous phage Pf3 as determined by NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Secondary structure of the single-stranded DNA binding protein encoded by filamentous phage Pf3 as determined by NMR.
Eur J Biochem. 1994 Sep 1;224(2):663-76
Authors: Folmer RH, Folkers PJ, Kaan A, Jonker AJ, Aelen JM, Konings RN, Hilbers CW
Nuclear magnetic resonance...