Related ArticlesStructure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR.
J Vis Exp. 2013;(82)
Authors: Shoshan MS, Tshuva EY, Shalev DE
Abstract
Copper (I) binding by metallochaperone transport proteins prevents copper oxidation and release of the toxic ions that may participate in harmful redox reactions. The Cu (I) complex of the peptide model of a Cu (I) binding metallochaperone protein, which includes the sequence MTCSGCSRPG (underlined is conserved), was determined in solution under inert conditions by NMR spectroscopy. NMR is a widely accepted technique for the determination of solution structures of proteins and peptides. Due to difficulty in crystallization to provide single crystals suitable for X-ray crystallography, the NMR technique is extremely valuable, especially as it provides information on the solution state rather than the solid state. Herein we describe all steps that are required for full three-dimensional structure determinations by NMR. The protocol includes sample preparation in an NMR tube, 1D and 2D data collection and processing, peak assignment and integration, molecular mechanics calculations, and structure analysis. Importantly, the analysis was first conducted without any preset metal-ligand bonds, to assure a reliable structure determination in an unbiased manner.
[NMR paper] Insight into Magnesium Coordination Environments in Benzoate and Salicylate Complexes through (25)Mg Solid-State NMR Spectroscopy.
Insight into Magnesium Coordination Environments in Benzoate and Salicylate Complexes through (25)Mg Solid-State NMR Spectroscopy.
Insight into Magnesium Coordination Environments in Benzoate and Salicylate Complexes through (25)Mg Solid-State NMR Spectroscopy.
J Phys Chem A. 2013 Jul 8;
Authors: Burgess KM, Xu Y, Leclerc MC, Bryce DL
Abstract
We report on the 25Mg solid-state nuclear magnetic resonance (NMR) characterization of a series of magnesium complexes featuring Mg2+ ions in organic coordination environments. Six compounds have...
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[NMR paper] NMR studies on Cu(II)-peptide complexes: exchange kinetics and determination of struc
NMR studies on Cu(II)-peptide complexes: exchange kinetics and determination of structures in solution.
Related Articles NMR studies on Cu(II)-peptide complexes: exchange kinetics and determination of structures in solution.
Mol Biosyst. 2005 May;1(1):79-84
Authors: Gaggelli E, Kozlowski H, Valensin D, Valensin G
The interaction of copper(II) with histidine containing peptides has recently acquired renewed interest following the established link between abnormal protein behaviour in neurodegenerative processes and unpaired copper homeostasis....
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11-24-2010 11:14 PM
[NMR paper] Structure determination of protein/RNA complexes by NMR.
Structure determination of protein/RNA complexes by NMR.
Related Articles Structure determination of protein/RNA complexes by NMR.
Methods Enzymol. 2005;394:525-45
Authors: Wu H, Finger LD, Feigon J
Structure determination of protein?RNA complexes in solution provides unique insights into factors that are involved in protein/RNA recognition. Here, we review the methodology used in our laboratory to overcome the challenges of protein?RNA structure determination by nuclear magnetic resonance (NMR). We use as two examples complexes recently...
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[NMR paper] Determination of the electron relaxation rates in paramagnetic metal complexes: appli
Determination of the electron relaxation rates in paramagnetic metal complexes: applicability of available NMR methods.
Related Articles Determination of the electron relaxation rates in paramagnetic metal complexes: applicability of available NMR methods.
J Magn Reson. 2004 Apr;167(2):169-77
Authors: Jensen MR, Led JJ
Four different approaches for determining the electron relaxation rates in paramagnetic metallo-proteins are investigated, using a paramagnetic Ni2+ complex of a protein as an example. All four approaches rely on the...
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11-24-2010 09:51 PM
[NMR paper] Structure determination of protein complexes by NMR.
Structure determination of protein complexes by NMR.
Related Articles Structure determination of protein complexes by NMR.
Methods Mol Biol. 2004;278:255-88
Authors: Nietlispach D, Mott HR, Stott KM, Nielsen PR, Thiru A, Laue ED
This chapter describes nuclear magnetic resonance (NMR) methods that can be used to determine the structures of protein complexes. Many of these techniques are also applicable to other systems (e.g., protein-nucleic acid complexes). In the first section, we discuss methodologies for optimizing the sample conditions for...
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[NMR paper] NMR structure determination of proteins and protein complexes larger than 20 kDa.
NMR structure determination of proteins and protein complexes larger than 20 kDa.
Related Articles NMR structure determination of proteins and protein complexes larger than 20 kDa.
Curr Opin Chem Biol. 1998 Oct;2(5):564-70
Authors: Clore GM, Gronenborn AM
Recent advances in multidimensional nuclear magnetic resonance methodology to obtain 1H, 15N and 13C resonance assignments, interproton distance and torsion angle restraints, and restraints that characterize long-range order, coupled with new methods of structure refinement and novel methods...
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11-17-2010 11:15 PM
PCS-based structure determination of proteinâ??protein complexes
Abstract A simple and fast nuclear magnetic resonance method for docking proteins using pseudo-contact shift (PCS) and 1HN/15N chemical shift perturbation is presented. PCS is induced by a paramagnetic lanthanide ion that is attached to a target protein using a lanthanide binding peptide tag anchored at two points. PCS provides long-range (~40 Ã?) distance and angular restraints between the lanthanide ion and the observed nuclei, while the 1HN/15N chemical shift perturbation data provide loose contact-surface information. The usefulness of this method was demonstrated through the structure...
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Structure Determination of Protein Complexes by NMR
Structure Determination of Protein Complexes by NMR
D. Nietlispach, H.R. Mott, K.M. Stott, P.R. Nielsen, A. Thiru & E.D. Laue
The Department of Biochemistry, University of Cambridge
Introduction
As the structures of more proteins and domains are solved by structural genomics projects, the future of structural biology will be oriented more toward the study of macromolecular complexes. Since so many biological processes are mediated by interactions between proteins, it is important to study them at a molecular level. The study of protein-protein interactions also has applications in a...
Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR.
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