A Structure-BasedAssembly Screen of Protein CageLibraries in Living Cells: Experimentally Repacking a Protein–ProteinInterface To Recover Cage Formation in an Assembly-Frustrated Mutant
A Structure-BasedAssembly Screen of Protein CageLibraries in Living Cells: Experimentally Repacking a Protein–ProteinInterface To Recover Cage Formation in an Assembly-Frustrated Mutant
[NMR paper] 3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy.
Chem Commun (Camb). 2016 Jul 29;
Authors: Pan BB, Yang F, Ye Y, Wu Q, Li C, Huber T, Su XC
Abstract
Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of...
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Detection of RNA–Protein Interactions in Living Cells with SHAPE
Detection of RNA–Protein Interactions in Living Cells with SHAPE
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.5b00977/20151111/images/medium/bi-2015-00977x_0007.gif
Biochemistry
DOI: 10.1021/acs.biochem.5b00977
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/WwxMghExNKY
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[NMR paper] Protein dynamics in living cells studied by in-cell NMR spectroscopy.
Protein dynamics in living cells studied by in-cell NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein dynamics in living cells studied by in-cell NMR spectroscopy.
FEBS Lett. 2013 Jan 11;
Authors: Li C, Liu M
Abstract
Most proteins function in cells where protein concentrations can reach 400g/l. However, most quantitative studies of protein properties are performed in idealized, dilute conditions. Recently developed in-cell NMR techniques...
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02-03-2013 10:22 AM
Protein dynamics in living cells studied by in-cell NMR spectroscopy
Protein dynamics in living cells studied by in-cell NMR spectroscopy
Available online 11 January 2013
Publication year: 2013
Source:FEBS Letters</br>
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Most proteins function in cells where protein concentrations can reach 400g/l. However, most quantitative studies of protein properties are performed in idealized, dilute conditions. Recently developed in-cell NMR techniques can provide protein structure and other biophysical properties inside living cells at atomic resolution. Here we review how protein dynamics, including global and internal motions have been...
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02-03-2013 10:13 AM
Watching protein structure at work in living cells using NMR spectroscopy
Watching protein structure at work in living cells using NMR spectroscopy
December 2012
Publication year: 2012
Source:Current Opinion in Chemical Biology, Volume 16, Issues 5–6</br>
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Isotope-assisted multi-dimensional NMR spectroscopy can now be applied to proteins inside living cells. The technique, called in-cell NMR, aims to investigate the structures, interactions and dynamics of proteins under their native conditions, ideally at an atomic resolution. The application has begun with bacterial cells but has now expanded to mammalian cultured cells, such as HeLa...
A Structure-BasedAssembly Screen of Protein CageLibraries in Living Cells: Experimentally Repacking a Protein–ProteinInterface To Recover Cage Formation in an Assembly-Frustrated Mutant
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