The cyclodepsipeptide FR900359 (FR) and its analogs are able to selectively inhibit the class of G(q) proteins by blocking GDP/GTP exchange. The inhibitor binding site of G(q) has been characterized by X-ray crystallography, and various binding and functional studies have determined binding kinetics and mode of inhibition. Here we investigate isotope-labeled FR bound to the membrane-anchored G protein heterotrimer by solid-state nuclear magnetic resonance (ssNMR) and in solution by liquid-state...
[NMR paper] Escherichia coli topoisomerase IV E subunit and an inhibitor binding mode revealed by NMR spectroscopy.
Escherichia coli topoisomerase IV E subunit and an inhibitor binding mode revealed by NMR spectroscopy.
Escherichia coli topoisomerase IV E subunit and an inhibitor binding mode revealed by NMR spectroscopy.
J Biol Chem. 2016 Jun 30;
Authors: Li Y, Wong YL, Ng FM, Liu B, Wong YX, Poh ZY, Liu S, Then SW, Lee MY, Ng HQ, Huang Q, Hung AW, Cherian J, Hill J, Keller TH, Kang C
Abstract
Bacterial topoisomerases are attractive antibacterial drug targets due to their importance in bacterial growth and low homology with other human...
[NMR paper] Lipid binding protein response to a bile acid library: a combined NMR and statistical approach.
Lipid binding protein response to a bile acid library: a combined NMR and statistical approach.
Related Articles Lipid binding protein response to a bile acid library: a combined NMR and statistical approach.
FEBS J. 2015 Aug 11;
Authors: Tomaselli S, Pagano K, Boulton S, Zanzoni S, Melacini G, Molinari H, Ragona L
Abstract
Primary bile acids, differing in the hydroxylation pattern, are synthesized from cholesterol in the liver and, once formed, can undergo extensive enzyme-catalyzed glycine/taurine conjugation, giving rise to a...
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[NMR paper] A novel 2-oxoindolinylidene inhibitor of bacterial MurD ligase: Enzyme kinetics, protein-inhibitor binding by NMR and a molecular dynamics study.
A novel 2-oxoindolinylidene inhibitor of bacterial MurD ligase: Enzyme kinetics, protein-inhibitor binding by NMR and a molecular dynamics study.
Related Articles A novel 2-oxoindolinylidene inhibitor of bacterial MurD ligase: Enzyme kinetics, protein-inhibitor binding by NMR and a molecular dynamics study.
Eur J Med Chem. 2014 Jun 11;83C:92-101
Authors: Sim?i? M, Pureber K, Kristan K, Urleb U, Kocjan D, Grdadolnik SG
Abstract
N-(5-(5-nitro-2-oxo-1,2-dihydro-3H-indol-3-ylidene)4-oxo-2-thioxo-1,3-thiazolidin-3-yl)nicotinamide,...
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A Novel 2-oxoindolinylidene Inhibitor of Bacterial MurD Ligase: Enzyme Kinetics, Protein-Inhibitor Binding by NMR and a Molecular Dynamics Study
A Novel 2-oxoindolinylidene Inhibitor of Bacterial MurD Ligase: Enzyme Kinetics, Protein-Inhibitor Binding by NMR and a Molecular Dynamics Study
Publication date: Available online 11 June 2014
Source:European Journal of Medicinal Chemistry</br>
Author(s): Mihael Sim?i? , Kaja Pureber , Katja Kristan , Uroš Urleb , Darko Kocjan , Simona Goli? Grdadolnik</br>
N-(5-(5-nitro-2-oxo-1,2-dihydro-3H-indol-3-ylidene)4-oxo-2-thioxo-1,3-thiazolidin-3-yl)nicotinamide, an 2-oxoindolinylidene derivative with novel structure scaffold, was evaluated for inhibition potency...
Calcium binding environments probed by (43)Ca NMR spectroscopy.
Calcium binding environments probed by (43)Ca NMR spectroscopy.
Calcium binding environments probed by (43)Ca NMR spectroscopy.
Dalton Trans. 2010 Oct 7;39(37):8593-602
Authors: Bryce DL
Calcium is an important component of materials, metalloproteins, minerals, glasses, and small inorganic and organic complexes. However, NMR spectroscopy of the quadrupolar (43)Ca nuclide remains difficult primarily due to its low natural abundance and low resonance frequency. In this Perspective, experimental challenges and recent successes in the field are...
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[NMR paper] The insulin-like growth factor (IGF)binding protein 1 binding epitope on IGF-I probed
The insulin-like growth factor (IGF)binding protein 1 binding epitope on IGF-I probed by heteronuclear NMR spectroscopy and mutational analysis.
Related Articles The insulin-like growth factor (IGF)binding protein 1 binding epitope on IGF-I probed by heteronuclear NMR spectroscopy and mutational analysis.
J Biol Chem. 1998 Sep 18;273(38):24701-7
Authors: Jansson M, Andersson G, Uhlén M, Nilsson B, Kördel J
NMR spectroscopy studies and biosensor interaction analysis of native and site-directed mutants of insulin-like growth factor I (IGF-I) was...
Structural response of G protein binding to the cyclodepsipeptide inhibitor FR900359 probed by NMR spectroscopy
Linear Mode
