Related ArticlesStructural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy.
Bioorg Med Chem. 2017 Dec 15;25(24):6589-6596
Authors: Calvanese L, Focà A, Sandomenico A, Focà G, Caporale A, Doti N, Iaccarino E, Leonardi A, D'Auria G, Ruvo M, Falcigno L
Abstract
Nodal is a growth factor expressed during early embryonic development, but reactivated in several advanced-stage cancers. Targeting of Nodal signaling, which occurs via the binding to Cripto-1 co-receptor, results in inhibition of cell aggressiveness and reduced tumor growth. The Nodal binding region to Cripto-1 was identified and targeted with a high affinity monoclonal antibody (3D1). By STD-NMR technique, we investigated the interaction of Nodal fragments with 3D1 with the aim to elucidate at atomic level the interaction surface. Data indicate with high accuracy the antibody-antigen contact atoms and confirm the information previously obtained by immune-enzymatic methods. Main residues contacted by 3D1 are P46, V47, E49 and E50, which belong to the Nodal loop involved in the interaction with the co-receptor.
[NMR paper] Structural insight into the XTACC3/XMAP215 interaction from CD and NMR studies on model peptides.
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Biopolymers. 2017 Sep 18;:
Authors: Partida-Hanon A, Treviño MA, Mompeán M, Jiménez MÁ, Bruix M
Abstract
TACC3 is a centrosomal adaptor protein that plays important roles during mitotic spindle assembly. It interacts with chTOG/XMAP215, which catalyzes the addition of tubulin dimers during microtubule growth. A 3D...
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[NMR paper] Structural and Dynamic Insights of the Interaction between Tritrpticin and Micelles: An NMR Study.
Structural and Dynamic Insights of the Interaction between Tritrpticin and Micelles: An NMR Study.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif Related Articles Structural and Dynamic Insights of the Interaction between Tritrpticin and Micelles: An NMR Study.
Biophys J. 2016 Dec 20;111(12):2676-2688
Authors: Santos TL, Moraes A, Nakaie CR, Almeida FC, Schreier S, Valente AP
Abstract
A large number of antimicrobial peptides (AMPs) acts with high selectivity...
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[NMR paper] Investigating liquid-liquid phase separation of a monoclonal antibody using solution-state NMR spectroscopy: effect of Arg·Glu and Arg·HCl.
Investigating liquid-liquid phase separation of a monoclonal antibody using solution-state NMR spectroscopy: effect of Arg·Glu and Arg·HCl.
Investigating liquid-liquid phase separation of a monoclonal antibody using solution-state NMR spectroscopy: effect of Arg·Glu and Arg·HCl.
Mol Pharm. 2017 Jun 14;:
Authors: Kheddo P, Bramham JE, Dearman RJ, Uddin S, van der Walle CF, Golovanov AP
Abstract
Liquid-liquid phase separation (LLPS) of monoclonal antibody (mAb) formulations involves spontaneous separation into dense...
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[NMR paper] Characterizing monoclonal antibody formulations in arginine glutamate solutions using (1)H NMR spectroscopy.
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MAbs. 2016 Aug 11;:1-14
Authors: Kheddo P, Cliff MJ, Uddin S, van der Walle CF, Golovanov AP
Abstract
Assessing how excipients affect the self-association of monoclonal antibodies (mAbs) requires informative and direct in situ measurements for highly concentrated solutions, without sample dilution...
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09-03-2016 05:38 PM
[NMR paper] Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure.
Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure.
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J Pharm Biomed Anal. 2016 Jun 7;128:398-407
Authors: Chen K, Long DS, Lute SC, Levy MJ, Brorson KA, Keire DA
Abstract
Monoclonal antibody (mAb) drugs constitute the largest class of protein therapeutics currently on the market. Correctly folded protein higher order structure...
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06-28-2016 06:25 PM
[NMR paper] Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Mapping monoclonal antibody structure by 2D 13C NMR at natural abundance.
Anal Chem. 2015 Apr 7;87(7):3556-61
Authors: Arbogast LW, Brinson RG, Marino JP
Abstract
Monoclonal antibodies (mAbs) represent an important and rapidly growing class of biotherapeutics. Correct folding of a mAb is critical for drug efficacy, while misfolding...
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[NMR paper] Unambiguous characterization of N-glycans of monoclonal antibody cetuximab by integration of LC-MS/MS and ¹H NMR spectroscopy.
Unambiguous characterization of N-glycans of monoclonal antibody cetuximab by integration of LC-MS/MS and ¹H NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Unambiguous characterization of N-glycans of monoclonal antibody cetuximab by integration of LC-MS/MS and ¹H NMR spectroscopy.
Anal Chem. 2014 May 20;86(10):4807-14
Authors: Wiegandt A, Meyer B
Abstract
Monoclonal antibodies are most rapidly emerging as therapeutic drugs for...
Structural insights into the interaction of a monoclonal antibody and Nodal peptides by STD-NMR spectroscopy.
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