PURPOSE: Antisense oligonucleotide (ASO) therapeutics are an emerging class of biopharmaceuticals to treat and prevent diseases, particularly those involving "undruggable" protein targets. Impurities generated throughout the ASO drug manufacturing and formulation pipeline can be detrimental to drug safety and efficacy. Therefore, analytical techniques are needed to rigorously characterize these molecules for quality assurance purposes.
[NMR paper] GPCR structural characterization by NMR spectroscopy in solution
GPCR structural characterization by NMR spectroscopy in solution
In the human proteome, 826 G-protein-coupled receptors (GPCRs) interact with extracellular stimuli to initiate cascades of intracellular signaling. Determining conformational dynamics and intermolecular interactions are key to understand GPCR function as a basis for drug design. X-ray crystallography and cryo-electron microscopy (cryo-EM) contribute molecular architectures of GPCRs and GPCR-signaling complexes. NMR spectroscopy is complementary by providing information on the dynamics of GPCR...
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[NMR paper] High Resolution NMR Spectroscopy as a Structural and Analytical Tool for Unsaturated Lipids in Solution.
High Resolution NMR Spectroscopy as a Structural and Analytical Tool for Unsaturated Lipids in Solution.
High Resolution NMR Spectroscopy as a Structural and Analytical Tool for Unsaturated Lipids in Solution.
Molecules. 2017 Oct 05;22(10):
Authors: Alexandri E, Ahmed R, Siddiqui H, Choudhary MI, Tsiafoulis CG, Gerothanassis IP
Abstract
Mono- and polyunsaturated lipids are widely distributed in Nature, and are structurally and functionally a diverse class of molecules with a variety of physicochemical, biological, medicinal and...
[NMR paper] On the Analytical Superiority of 1D NMR for Fingerprinting the Higher Order Structure of Protein Therapeutics Compared to Multidimensional NMR Methods.
On the Analytical Superiority of 1D NMR for Fingerprinting the Higher Order Structure of Protein Therapeutics Compared to Multidimensional NMR Methods.
Related Articles On the Analytical Superiority of 1D NMR for Fingerprinting the Higher Order Structure of Protein Therapeutics Compared to Multidimensional NMR Methods.
Anal Chem. 2015 Apr 30;
Authors: Poppe L, Jordan JB, Rogers G, Schnier PD
Abstract
An important aspect in the analytical characterization of protein therapeutics is the comprehensive characterization of higher...
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[NMR paper] NMR Characterization of an Oligonucleotide Model of the MiR-21 Pre-Element.
NMR Characterization of an Oligonucleotide Model of the MiR-21 Pre-Element.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.plosone.org-images-pone_120x30.png Related Articles NMR Characterization of an Oligonucleotide Model of the MiR-21 Pre-Element.
PLoS One. 2014;9(9):e108231
Authors: Chirayil S, Wu Q, Amezcua C, Luebke KJ
Abstract
We have used NMR spectroscopy to characterize an oligonucleotide stem loop structure based on the pre-element of an oncogenic microRNA, miR-21. This predicted stem-loop...
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09-25-2014 11:57 PM
[NMR paper] High-resolution NMR of an antisense DNA x RNA hybrid containing alternating chirally
High-resolution NMR of an antisense DNA x RNA hybrid containing alternating chirally pure Rp methylphosphonates in the DNA backbone.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles High-resolution NMR of an antisense DNA x RNA hybrid containing alternating chirally pure Rp methylphosphonates in the DNA backbone.
Biochemistry. 1997 Mar 4;36(9):2371-9
Authors: Mujeeb A, Reynolds MA, James TL
A high-resolution proton NMR study has been performed on a hybrid duplex formed by a...
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08-22-2010 03:31 PM
[NMR paper] High-resolution NMR of an antisense DNA x RNA hybrid containing alternating chirally
High-resolution NMR of an antisense DNA x RNA hybrid containing alternating chirally pure Rp methylphosphonates in the DNA backbone.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles High-resolution NMR of an antisense DNA x RNA hybrid containing alternating chirally pure Rp methylphosphonates in the DNA backbone.
Biochemistry. 1997 Mar 4;36(9):2371-9
Authors: Mujeeb A, Reynolds MA, James TL
A high-resolution proton NMR study has been performed on a hybrid duplex formed by a...
Structural Fingerprinting of Antisense Oligonucleotide Therapeutics by Solution NMR Spectroscopy
Linear Mode
