Related ArticlesStructural effects of hydration: studies of lysozyme by 13C solids NMR.
Biopolymers. 1990 Dec;29(14):1801-6
Authors: Kennedy SD, Bryant RG
13C-nmr spectra of lysozyme obtained at 50.3 MHz using both static and magic-angle-spinning-cross-polarization methods are reported at several water contents. The line widths and consequent resolution in the hydrated material is substantially improved over that in the lyophilized protein. The line narrowing is not commensurate with loss of a proton-carbon dipole-dipole coupling or dramatic changes in the relaxation parameters characterizing magnetization transfer from protons to carbon in the Hartmann-Hahn cross-polarization experiment. We interpret these data in terms of the water inducing a decrease in the distribution of local conformations sampled by the protein, although the magnitude of the conformational reorientations required to account for the data are not necessarily large nor do they imply a major unfolding of the protein on dehydration.
NMR studies of alkali metal ions in organic and biological solids
NMR studies of alkali metal ions in organic and biological solids
Publication year: 2011
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 13 June 2011</br>
Gang, Wu , Jianfeng, Zhu</br>
*Highlights:*? Comprehensive review of NMR of alkali metal ions in organic/biological solids ? Experimental solid-state NMR techniques and computational methods ? Survey of experimental alkali metal NMR data for organic/biological solids</br></br>
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The effects of anticalcification treatments and hydration on the molecular dynamics of bovine pericardium collagen as revealed by 13C solid-state NMR.
The effects of anticalcification treatments and hydration on the molecular dynamics of bovine pericardium collagen as revealed by 13C solid-state NMR.
The effects of anticalcification treatments and hydration on the molecular dynamics of bovine pericardium collagen as revealed by 13C solid-state NMR.
Magn Reson Chem. 2010 Sep;48(9):704-11
Authors: deAzevedo ER, Ayrosa AM, Faria GC, Cervantes HJ, Huster D, Bonagamba TJ, Pitombo RN, Rabbani SR
This article describes a solid-state NMR (SSNMR) investigation of the influence of hydration and chemical...
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01-21-2011 12:00 PM
[NMR paper] Secondary structural effects on protein NMR chemical shifts.
Secondary structural effects on protein NMR chemical shifts.
Related Articles Secondary structural effects on protein NMR chemical shifts.
J Biomol NMR. 2004 Nov;30(3):233-44
Authors: Wang Y
For an amino acid in protein, its chemical shift, delta(phi, psi)(s), is expressed as a function of its backbone torsion angles (phi and psi) and secondary state (s): delta(phi, psi)(s=deltaphi, psi)_coil+Deltadelta(phi, psi)_s), where delta(phi, psi)(coil) represents its chemical shift at coil state (s=coil); Delta delta(phi, psi)(s) (s=sheet or helix) is...
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[NMR paper] Solid-state NMR studies of pharmaceutical solids in polymer matrices.
Solid-state NMR studies of pharmaceutical solids in polymer matrices.
Related Articles Solid-state NMR studies of pharmaceutical solids in polymer matrices.
Anal Bioanal Chem. 2004 Mar;378(6):1504-10
Authors: Lubach JW, Padden BE, Winslow SL, Salsbury JS, Masters DB, Topp EM, Munson EJ
Biodegradable drug-delivery systems can be formulated to release drug for hours to years and have been used for the controlled release of medications in animals and humans. An important consideration in developing a drug-delivery matrix is knowledge of the...
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11-24-2010 09:25 PM
[NMR paper] Solid-state (13)C NMR reveals effects of temperature and hydration on elastin.
Solid-state (13)C NMR reveals effects of temperature and hydration on elastin.
Related Articles Solid-state (13)C NMR reveals effects of temperature and hydration on elastin.
Biophys J. 2002 Feb;82(2):1086-95
Authors: Perry A, Stypa MP, Tenn BK, Kumashiro KK
Elastin is the principal protein component of the elastic fiber in vertebrate tissue. The waters of hydration in the elastic fiber are believed to play a critical role in the structure and function of this largely hydrophobic, amorphous protein. (13)C CPMAS NMR spectra are acquired for...
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[NMR paper] NMR studies of protein hydration.
NMR studies of protein hydration.
Related Articles NMR studies of protein hydration.
Prog Biophys Mol Biol. 1994;61(1):61-79
Authors: Belton PS
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[NMR paper] NMR studies of protein hydration.
NMR studies of protein hydration.
Related Articles NMR studies of protein hydration.
Prog Biophys Mol Biol. 1994;61(1):61-79
Authors: Belton PS
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[NMR paper] The influence of hydration on the conformation of lysozyme studied by solid-state 13C
The influence of hydration on the conformation of lysozyme studied by solid-state 13C-NMR spectroscopy.
Related Articles The influence of hydration on the conformation of lysozyme studied by solid-state 13C-NMR spectroscopy.
Biopolymers. 1993 Apr;33(4):513-9
Authors: Gregory RB, Gangoda M, Gilpin RK, Su W
13C proton decoupled cross-polarization magic-angle spinning nmr spectra of lysozyme are reported as a function of hydration. Increases in hydration level enhance the resolution of the spectra, particularly in the aliphatic region, but has no...
Structural effects of hydration: studies of lysozyme by 13C solids NMR.
Linear Mode
