Related ArticlesStructural characterization of the molten globule and native states of ovalbumin: a 1H NMR study.
J Pept Res. 1997 Dec;50(6):465-74
Authors: Sogami M, Era S, Koseki T, Nagai N
Molecular characteristics of ovalbumin (OVA) in the acidic (pD 3.08, the E-form) and neutral [pD 7.29, the N-form (native form)] regions were studied by measuring effective radii, 1H NMR spectra, spin-echo 1H NMR spectra and cross-relaxation times (TIS) from irradiated to observed protein protons which are particularly sensitive for detection of the mobile segments and/or structural looseness in proteins. 1H NMR spectra did not show significant differences between the N- and E-forms except for the spectral lines in the CH3, epsilon CH2 and aromatic regions. Effective radii and TIS values for main- and side-chains showed 1.08 and 1.5- to 2.0-fold increases on going from the N- to E-forms, respectively. The elongation of TIS values might indicate the appearance of the fluctuating tertiary structure in the E-form. Molecular characteristics of the E-form, inferred from reported far ultraviolet-circular dichroism (UV-CD) spectra in the peptide region, near UV-CD spectra in the aromatic region [Koseki et al. (1988) J. Biochem. 103, 425-430], effective radii and especially elongation of TIS values might indicate that the E-form could be in the molten globule state. The onset of denaturation of OVA using TIS measurements was also studied.
[NMR paper] Water-protein interactions in the molten-globule state of carbonic anhydrase b: an NM
Water-protein interactions in the molten-globule state of carbonic anhydrase b: an NMR spin-diffusion study.
Related Articles Water-protein interactions in the molten-globule state of carbonic anhydrase b: an NMR spin-diffusion study.
Protein Sci. 2000 Aug;9(8):1540-7
Authors: Kutyshenko VP, Cortijo M
We have used the homonuclear Overhauser effect (NOE) to characterize a model protein: carbonic anhydrase B. We have obtained NOE difference spectra for this protein, centering the on-resonance signals either at the methyl-proton or at the...
nmrlearner
Journal club
0
11-19-2010 08:29 PM
[NMR paper] Side chain accessibility and dynamics in the molten globule state of alpha-lactalbumi
Side chain accessibility and dynamics in the molten globule state of alpha-lactalbumin: a (19)F-NMR study.
Related Articles Side chain accessibility and dynamics in the molten globule state of alpha-lactalbumin: a (19)F-NMR study.
Biochemistry. 2000 Jan 18;39(2):372-80
Authors: Bai P, Luo L, Peng Z
The molten globule state of alpha-lactalbumin (alpha-LA) has been considered a prototype of partially folded proteins. Despite the importance of molten globules in understanding the mechanisms of protein folding and its relevance to some biological...
nmrlearner
Journal club
0
11-18-2010 09:15 PM
[NMR paper] Populating the equilibrium molten globule state of apomyoglobin under conditions suit
Populating the equilibrium molten globule state of apomyoglobin under conditions suitable for structural characterization by NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Populating the equilibrium molten globule state of apomyoglobin under conditions suitable for structural characterization by NMR.
FEBS Lett. 1997 Nov 3;417(1):92-6
Authors: Eliezer D, Jennings PA, Dyson HJ, Wright PE
Conditions have been determined under which the equilibrium molten globule...
nmrlearner
Journal club
0
08-22-2010 05:08 PM
[NMR paper] A residue-specific NMR view of the non-cooperative unfolding of a molten globule.
A residue-specific NMR view of the non-cooperative unfolding of a molten globule.
Related Articles A residue-specific NMR view of the non-cooperative unfolding of a molten globule.
Nat Struct Biol. 1997 Aug;4(8):630-4
Authors: Schulman BA, Kim PS, Dobson CM, Redfield C
Molten globules are partially folded forms of proteins that are thought to be general intermediates in protein folding. Nonetheless, there is limited structural information about such species because they possess conformational heterogeneity and complex dynamical properties that...
nmrlearner
Journal club
0
08-22-2010 05:08 PM
[NMR paper] Structural analysis of non-native states of proteins by NMR methods.
Structural analysis of non-native states of proteins by NMR methods.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Structural analysis of non-native states of proteins by NMR methods.
Curr Opin Struct Biol. 1996 Feb;6(1):24-30
Authors: Shortle DR
Established NMR methods are increasingly being applied to the non-native states of proteins. For small denatured proteins, full assignment of proton, 15N and 13C resonances is often straightforward. Sensitive methods exist...
nmrlearner
Journal club
0
08-22-2010 02:27 PM
[NMR paper] Is apomyoglobin a molten globule? Structural characterization by NMR.
Is apomyoglobin a molten globule? Structural characterization by NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Is apomyoglobin a molten globule? Structural characterization by NMR.
J Mol Biol. 1996 Nov 8;263(4):531-8
Authors: Eliezer D, Wright PE
Multi-dimensional heteronuclear NMR spectroscopy has been used to obtain structural information on isotopically labeled recombinant sperm whale apomyoglobin in the native state at pH 6.1. Assignments for backbone...
nmrlearner
Journal club
0
08-22-2010 02:20 PM
[NMR paper] Stability of alpha-helices in a molten globule state of cytochrome c by hydrogen-deut
Stability of alpha-helices in a molten globule state of cytochrome c by hydrogen-deuterium exchange and two-dimensional NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Stability of alpha-helices in a molten globule state of cytochrome c by hydrogen-deuterium exchange and two-dimensional NMR spectroscopy.
J Mol Biol. 1995 Apr 7;247(4):682-8
Authors: Kuroda Y, Endo S, Nagayama K, Wada A
To distinguish between intrinsically stable helices and those...
nmrlearner
Journal club
0
08-22-2010 03:41 AM
[NMR paper] Structure and dynamics of the acid-denatured molten globule state of alpha-lactalbumi
Structure and dynamics of the acid-denatured molten globule state of alpha-lactalbumin: a two-dimensional NMR study.
Related Articles Structure and dynamics of the acid-denatured molten globule state of alpha-lactalbumin: a two-dimensional NMR study.
Biochemistry. 1993 Feb 23;32(7):1707-18
Authors: Alexandrescu AT, Evans PA, Pitkeathly M, Baum J, Dobson CM
Two-dimensional 1H-NMR spectroscopy has been used to study the acid-denatured molten globule (A-state) of alpha-lactalbumin. The NMR spectra show that chemical shift dispersion is limited...