Related ArticlesStructural characterisation of macromolecular organic material in air particulate matter using Py-GC-MS and solid state 13C-NMR.
J Environ Monit. 2000 Dec;2(6):561-5
Authors: Subbalakshmi Y, Patti AF, Lee GS, Hooper MA
Organic air particulate matter was analysed by applying the techniques of Py-GC-MS (pyrolysis-gas chromatography-mass spectrometry) and solid state 13C-NMR (nuclear magnetic resonance). Particles dislodged from air particulate filters and humic acid extracted from these filters were studied for structural components. The structural components of the air particles and extracted humic acid consisted of compounds originating from biomacromolecules, namely, lignin, carbohydrates, protein and lipids. The main components identified for each class included: (1) methoxyphenols originating from lignin; (2) furans, aldehydes and ketones from carbohydrates; (3) pyrrole, indoles from protein; and (4) many hydrocarbons from lipid structures. Single ion monitoring (SIM) and tetramethyl ammonium hydroxide (TMAH) methylation were utilised for detection of aliphatic hydrocarbons and acidic components, respectively. Hydrocarbons ranging from C9 to C28 were detected by SIM analysis, while aliphatic acids ranged from C9 to C18. The majority of components analysed directly in the air particles were similar to those from the humic acid extracts. Many of the structural components of air particles were typical of humic substances of soil and aqueous systems and these were attributed to both biogenic and anthropogenic sources.
Structural Characterisation of a Histone Domain by Projection-Decomposition
Structural Characterisation of a Histone Domain by Projection-Decomposition
Publication year: 2012
Source: Journal of Magnetic Resonance, Available online 23 February 2012</br>
Jonas*Fredriksson, Wolfgang*Bermel, Martin*Billeter</br>
We demonstrate that two projection experiments, aN-HSQC–NOESY–N-HSQC and aC-HSQC–NOESY–N-HSQC, recorded for a histone domain from yeast, contain enough information to support a structural characterisation of the protein. At the temperature used, 298K, the histone domain exhibits a very high extent of chemical shift degeneracy that is uncharacteristic for...
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02-25-2012 04:20 PM
Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination.
Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination.
Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination.
FEBS J. 2011 Jan 7;
Authors: Bieri M, Kwan AH, Mobli M, King GF, Mackay JP, Gooley PR
A strength of NMR spectroscopy is its ability to monitor, on an atomic level, molecular changes and interactions. In this article, which is intended for non-spectroscopists, we describe major uses of NMR in protein science beyond...
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01-11-2011 11:27 PM
Evolution of organic matter during composting of different organic wastes assessed by
Evolution of organic matter during composting of different organic wastes assessed by CPMAS (13)C NMR spectroscopy.
Related Articles Evolution of organic matter during composting of different organic wastes assessed by CPMAS (13)C NMR spectroscopy.
Waste Manag. 2010 Oct 19;
Authors: Caricasole P, Provenzano MR, Hatcher PG, Senesi N
In this paper, the evolution of organic matter (OM) during composting of different mixtures of various organic wastes was assessed by means of chemical analyses and CPMAS (13)C NMR spectroscopy measured during...
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10-23-2010 05:48 PM
[BMNRC community] NMR material by Rezwave Technology Inc.
NMR material by Rezwave Technology Inc.
http://www.rezwave.com.tw/main-eng.htm
Go to BMNRC community to find more info about this topic.
nmrlearner
News from other NMR forums
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08-26-2010 06:37 AM
[NMR paper] Cold denaturation of barstar: 1H, 15N and 13C NMR assignment and characterisation of
Cold denaturation of barstar: 1H, 15N and 13C NMR assignment and characterisation of residual structure.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Cold denaturation of barstar: 1H, 15N and 13C NMR assignment and characterisation of residual structure.
J Mol Biol. 1996 Jun 21;259(4):805-18
Authors: Wong KB, Freund SM, Fersht AR
Detection of residual structure in denatured proteins is of interest because fleetingly structured regions may be initiation points of the...
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08-22-2010 02:27 PM
[NMR paper] Solution characterisation by NMR spectroscopy of two horseradish peroxidase isoenzyme
Solution characterisation by NMR spectroscopy of two horseradish peroxidase isoenzyme C mutants with alanine replacing either Phe142 or Phe143.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Solution characterisation by NMR spectroscopy of two horseradish peroxidase isoenzyme C mutants with alanine replacing either Phe142 or Phe143.
Eur J Biochem. 1995 Oct 15;233(2):650-8
Authors: Veitch NC, Williams RJ, Bone NM, Burke JF, Smith AT
...
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08-22-2010 03:50 AM
Michael Sattler's teaching material
The website of EMBL's Sattler Group contains links to a range of teaching materials:
http://www.embl-heidelberg.de/nmr/sattler/teaching/index.html
There's a lot of useful stuff on their website, but it's pretty difficult to navigate.
The website also has links to some of Claudia Muhle-Goll's biomolecular NMR lectures (PowerPoint):
Lecture 1: Phenomenon of NMR - why do we observe a signal?
Lecture 4: Labelling techniques and heteronuclear backbone assignment
Lecture 5: Product operator formalism
jen
Educational web pages
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09-02-2008 05:01 PM
NMR Spectroscopy Explained: Simplified Theory, Applications and Examples for Organic Chemistry and Structural Biology - Thomas L. James (2007)
NMR Spectroscopy Explained: Simplified Theory, Applications and Examples for Organic Chemistry and Structural Biology
Edited by Neil E. Jacobsen (2007)
Amazon book description
NMR Spectroscopy Explained : Simplified Theory, Applications and Examples for Organic Chemistry and Structural Biology provides a fresh, practical guide to NMR for both students and practitioners, in a clearly written and non-mathematical format. It gives the reader an intermediate level theoretical basis for understanding laboratory applications, developing concepts gradually within the context of examples and...
Structural characterisation of macromolecular organic material in air particulate mat
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