Publication year: 2012 Source:Journal of Magnetic Resonance
Jonas Fredriksson, Wolfgang Bermel, Martin Billeter
We demonstrate that two projection experiments, a 15N-HSQC–NOESY–15N-HSQC and a 13C-HSQC–NOESY–15N-HSQC, recorded for a histone domain from yeast, contain enough information to support a structural characterisation of the protein. At the temperature used, 298K, the histone domain exhibits a very high extent of chemical shift degeneracy that is uncharacteristic for a fully folded domain. Nonetheless, a structured core of 67 residues, which is formed by three ?-helices and a two-stranded ?-sheet is defined by this NOESY data; this core structure was shown earlier to be present at lower temperature. The above two experiments, which together required 18 hours of instrument time, are part of a set of five projection experiments acquired during 2.5 days with the goal of complete characterisation of proteins, including full resonance assignment and structure. Graphical Abstract
Graphical abstract Highlights
? Protein structures from projection-decomposition data. ? Complete protein characterisation in 2.5 days of measurements. ? NOESY data for a highly flexible histone domain. ? Correlation between NOESY shapes and protein 3D structure.
Structural Characterisation of a Histone Domain by Projection-Decomposition
Structural Characterisation of a Histone Domain by Projection-Decomposition
Publication year: 2012
Source: Journal of Magnetic Resonance, Available online 23 February 2012</br>
Jonas*Fredriksson, Wolfgang*Bermel, Martin*Billeter</br>
We demonstrate that two projection experiments, aN-HSQC–NOESY–N-HSQC and aC-HSQC–NOESY–N-HSQC, recorded for a histone domain from yeast, contain enough information to support a structural characterisation of the protein. At the temperature used, 298K, the histone domain exhibits a very high extent of chemical shift degeneracy that is uncharacteristic for...
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Analysis of non-uniformly sampled spectra with Multi-Dimensional Decomposition
Analysis of non-uniformly sampled spectra with Multi-Dimensional Decomposition
Publication year: 2011
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 24 February 2011</br>
Vladislav Yu., Orekhov , Victor A., Jaravine</br>
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02-26-2011 01:07 PM
[NMR paper] Accuracy and robustness of three-way decomposition applied to NMR data.
Accuracy and robustness of three-way decomposition applied to NMR data.
Related Articles Accuracy and robustness of three-way decomposition applied to NMR data.
J Magn Reson. 2005 Jun;174(2):188-99
Authors: Luan T, Orekhov VY, Gutmanas A, Billeter M
Three-way decomposition is a very versatile analysis tool with applications in a variety of protein NMR fields. It has been used to extract structural data from 3D NOESYs, to determine relaxation rates in large proteins, to identify ligand binding in screening for lead compounds, and to complement...
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11-25-2010 08:21 PM
[NMR paper] Projection-reconstruction technique for speeding up multidimensional NMR spectroscopy
Projection-reconstruction technique for speeding up multidimensional NMR spectroscopy.
Related Articles Projection-reconstruction technique for speeding up multidimensional NMR spectroscopy.
J Am Chem Soc. 2004 May 26;126(20):6429-40
Authors: Kupce E, Freeman R
The acquisition of multidimensional NMR spectra can be speeded up by a large factor by a projection-reconstruction method related to a technique used in X-ray scanners. The information from a small number of plane projections is used to recreate the full multidimensional spectrum in the...
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11-24-2010 09:51 PM
[NMR paper] Projection-reconstruction of three-dimensional NMR spectra.
Projection-reconstruction of three-dimensional NMR spectra.
Related Articles Projection-reconstruction of three-dimensional NMR spectra.
J Am Chem Soc. 2003 Nov 19;125(46):13958-9
Authors: Kupce E, Freeman R
When three-dimensional NMR spectra are presented as two stereoscopic images, they create a convincing three-dimensional impression for the viewer. In an extension of this principle, we record plane projections of a three-dimensional spectrum at different angles, and use this limited information to reconstruct the entire spectrum....
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11-24-2010 09:16 PM
[NMR paper] Optimizing resolution in multidimensional NMR by three-way decomposition.
Optimizing resolution in multidimensional NMR by three-way decomposition.
Related Articles Optimizing resolution in multidimensional NMR by three-way decomposition.
J Biomol NMR. 2003 Oct;27(2):165-73
Authors: Orekhov VY, Ibraghimov I, Billeter M
Resolution depends on the number of points sampled in a FID; in indirectly detected dimensions it is an important determinant of the total experiment time. Based on the high redundancy present in NMR data, we propose the following timesaving scheme for three-dimensional spectra. An extensive grid of...
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11-24-2010 09:16 PM
[NMR paper] Structural characterisation of macromolecular organic material in air particulate mat
Structural characterisation of macromolecular organic material in air particulate matter using Py-GC-MS and solid state 13C-NMR.
Related Articles Structural characterisation of macromolecular organic material in air particulate matter using Py-GC-MS and solid state 13C-NMR.
J Environ Monit. 2000 Dec;2(6):561-5
Authors: Subbalakshmi Y, Patti AF, Lee GS, Hooper MA
Organic air particulate matter was analysed by applying the techniques of Py-GC-MS (pyrolysis-gas chromatography-mass spectrometry) and solid state 13C-NMR (nuclear magnetic...
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GFT projection NMR spectroscopy for proteins in the solid state.
GFT projection NMR spectroscopy for proteins in the solid state.
GFT projection NMR spectroscopy for proteins in the solid state.
J Biomol NMR. 2010 Oct 30;
Authors: Trent Franks W, Atreya HS, Szyperski T, Rienstra CM
Recording of four-dimensional (4D) spectra for proteins in the solid state has opened new avenues to obtain virtually complete resonance assignments and three-dimensional (3D) structures of proteins. As in solution state NMR, the sampling of three indirect dimensions leads per se to long minimal measurement time. Furthermore,...
Structural Characterisation of a Histone Domain by Projection-Decomposition
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