Related ArticlesStructural Analysis of Human Cofilin 2/Filamentous Actin Assemblies: Atomic-Resolution Insights from Magic Angle Spinning NMR Spectroscopy.
Sci Rep. 2017 Mar 17;7:44506
Authors: Yehl J, Kudryashova E, Reisler E, Kudryashov D, Polenova T
Abstract
Cellular actin dynamics is an essential element of numerous cellular processes, such as cell motility, cell division and endocytosis. Actin's involvement in these processes is mediated by many actin-binding proteins, among which the cofilin family plays unique and essential role in accelerating actin treadmilling in filamentous actin (F-actin) in a nucleotide-state dependent manner. Cofilin preferentially interacts with older filaments by recognizing time-dependent changes in F-actin structure associated with the hydrolysis of ATP and release of inorganic phosphate (Pi) from the nucleotide cleft of actin. The structure of cofilin on F-actin and the details of the intermolecular interface remain poorly understood at atomic resolution. Here we report atomic-level characterization by magic angle spinning (MAS) NMR of the muscle isoform of human cofilin 2 (CFL2) bound to F-actin. We demonstrate that resonance assignments for the majority of atoms are readily accomplished and we derive the intermolecular interface between CFL2 and F-actin. The MAS NMR approach reported here establishes the foundation for atomic-resolution characterization of a broad range of actin-associated proteins bound to F-actin.
[NMR paper] Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.
Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.
Related Articles Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.
Q Rev Biophys. 2017 Jan;50:e1
Authors: Quinn CM, Polenova T
Abstract
In recent years, exciting developments in instrument technology and experimental methodology have advanced the field of magic-angle spinning (MAS) nuclear magnetic resonance (NMR) to new heights. Contemporary MAS NMR yields atomic-level insights into structure and...
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01-19-2017 08:56 AM
[NMR paper] Atomic-resolution structure of the CAP-Gly domain of dynactin on polymeric microtubules determined by magic angle spinning NMR spectroscopy.
Atomic-resolution structure of the CAP-Gly domain of dynactin on polymeric microtubules determined by magic angle spinning NMR spectroscopy.
Atomic-resolution structure of the CAP-Gly domain of dynactin on polymeric microtubules determined by magic angle spinning NMR spectroscopy.
Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):14611-6
Authors: Yan S, Guo C, Hou G, Zhang H, Lu X, Williams JC, Polenova T
Abstract
Microtubules and their associated proteins perform a broad array of essential physiological functions, including...
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11-26-2015 12:22 PM
Magic Angle Spinning NMR Spectroscopy: A Versatile Technique for Structural and Dynamic Analysis of Solid-Phase Systems
From The DNP-NMR Blog:
Magic Angle Spinning NMR Spectroscopy: A Versatile Technique for Structural and Dynamic Analysis of Solid-Phase Systems
This review gives a comprehensive overview of the state-of-the-art of magic-angle spinning (MAS), solid-state NMR spectroscopy, including DNP-NMR spectroscopy.
1. Polenova, T., R. Gupta, and A. Goldbourt, Magic angle spinning NMR spectroscopy: a versatile technique for structural and dynamic analysis of solid-phase systems. Anal Chem, 2015. 87(11): p. 5458-69.
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06-29-2015 07:21 PM
[NMR paper] Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.
Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.
Correlating high-resolution magic angle spinning NMR spectroscopy and gene analysis in osteoarthritic cartilage.
NMR Biomed. 2015 Mar 12;
Authors: Tufts L, Shet Vishnudas K, Fu E, Kurhanewicz J, Ries M, Alliston T, Li X
Abstract
Osteoarthritis (OA) is a common multifactorial and heterogeneous degenerative joint disease, and biochemical changes in cartilage matrix occur during the early stages of OA before...
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03-13-2015 12:31 PM
[NMR paper] Magic Angle Spinning NMR Reveals Sequence-Dependent Structural Plasticity, Dynamics, and the Spacer Peptide 1 Conformation in HIV-1 Capsid Protein Assemblies.
Magic Angle Spinning NMR Reveals Sequence-Dependent Structural Plasticity, Dynamics, and the Spacer Peptide 1 Conformation in HIV-1 Capsid Protein Assemblies.
Magic Angle Spinning NMR Reveals Sequence-Dependent Structural Plasticity, Dynamics, and the Spacer Peptide 1 Conformation in HIV-1 Capsid Protein Assemblies.
J Am Chem Soc. 2013 Oct 28;
Authors: Han Y, Hou G, Suiter CL, Ahn J, Byeon IJ, Lipton AS, Burton SD, Hung I, Gor'kov PL, Gan Z, Brey WW, Rice D, Gronenborn AM, Polenova TE
Abstract
A key stage in HIV-1 maturation towards...
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10-30-2013 10:44 AM
[NMR paper] Broadband homonuclear correlation spectroscopy driven by combined R2n(v) sequences under fast magic angle spinning for NMR structural analysis of organic and biological solids.
Broadband homonuclear correlation spectroscopy driven by combined R2n(v) sequences under fast magic angle spinning for NMR structural analysis of organic and biological solids.
Related Articles Broadband homonuclear correlation spectroscopy driven by combined R2n(v) sequences under fast magic angle spinning for NMR structural analysis of organic and biological solids.
J Magn Reson. 2013 Apr 28;232C:18-30
Authors: Hou G, Yan S, Trébosc J, Amoureux JP, Polenova T
Abstract
We recently described a family of experiments for R2n(v) Driven Spin...
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05-21-2013 02:34 PM
[NMR paper] Probing Structure and Dynamics of Protein Assemblies by Magic Angle Spinning NMR Spectroscopy.
Probing Structure and Dynamics of Protein Assemblies by Magic Angle Spinning NMR Spectroscopy.
Probing Structure and Dynamics of Protein Assemblies by Magic Angle Spinning NMR Spectroscopy.
Acc Chem Res. 2013 Feb 13;
Authors: Yan S, Suiter CL, Hou G, Zhang H, Polenova T
Abstract
In living organisms, biological molecules often organize into multicomponent complexes. Such assemblies consist of various proteins and carry out essential functions, ranging from cell division, transport, and energy transduction to catalysis, signaling, and viral...
Structural Analysis of Human Cofilin 2/Filamentous Actin Assemblies: Atomic-Resolution Insights from Magic Angle Spinning NMR Spectroscopy.
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