Strategy for NMR metabolomic analysis of urine in mouse models of obesity- from sample collection to interpretation of acquired data.
J Pharm Biomed Anal. 2015 Jul 17;115:225-235
Authors: Pelantová H, Bugá?ová M, Anýž J, Železná B, Maletínská L, Novák D, Haluzík M, Kuzma M
Abstract
The mouse model of monosodium glutamate induced obesity was used to examine and consequently optimize the strategy for analysis of urine samples by NMR spectroscopy. A set of nineteen easily detectable metabolites typical in obesity-related studies was selected. The impact of urine collection protocol, choice of (1)H NMR pulse sequence, and finally the impact of the normalization method on the detected concentration of selected metabolites were investigated. We demonstrated the crucial effect of food intake and diurnal rhythms resulting in the choice of a 24-hour fasting collection protocol as the most convenient for tracking obesity-induced increased sensitivity to fasting. It was shown that the Carr-Purcell-Meiboom-Gill (CPMG) experiment is a better alternative to one-dimensional nuclear Overhauser enhancement spectroscopy (1D-NOESY) for NMR analysis of mouse urine due to its ability to filter undesirable signals of proteins naturally present in rodent urine. Normalization to total spectral area provided comparable outcomes as did normalization to creatinine or probabilistic quotient normalization in the CPMG-based model. The optimized approach was found to be beneficial mainly for low abundant metabolites rarely monitored due to their overlap by strong protein signals.
PMID: 26263053 [PubMed - as supplied by publisher]
Metabolomic Study Uncovers Obesity-related Urine Signature - GenomeWeb
<img alt="" height="1" width="1">
Metabolomic Study Uncovers Obesity-related Urine Signature
GenomeWeb
As part of the International Study of Macro- and Micronutrients and Blood Pressure, or INTERMAP, the team used a combination of nuclear magnetic resonance spectroscopy and ion exchange chromatography to track metabolite patterns in urine samples ...
and more »
Metabolomic Study Uncovers Obesity-related Urine Signature - GenomeWeb
More...
nmrlearner
Online News
0
04-30-2015 07:41 AM
[NMR paper] Prediction of response of collagen-induced arthritis rats to methotrexate: an (1)H-NMR-based urine metabolomic analysis.
Prediction of response of collagen-induced arthritis rats to methotrexate: an (1)H-NMR-based urine metabolomic analysis.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Prediction of response of collagen-induced arthritis rats to methotrexate: an (1)H-NMR-based urine metabolomic analysis.
J Huazhong Univ Sci Technolog Med Sci. 2012 Jun;32(3):438-43
Authors: Chen Z, Tu S, Hu Y, Wang Y, Xia Y, Jiang Y
Abstract
Over one half the...
nmrlearner
Journal club
0
09-12-2014 06:37 PM
[NMR paper] Gender-specific metabolomic profiling of obesity in leptin-deficient ob/ob mice by 1H NMR spectroscopy.
Gender-specific metabolomic profiling of obesity in leptin-deficient ob/ob mice by 1H NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.plosone.org-images-pone_120x30.png http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Gender-specific metabolomic profiling of obesity in leptin-deficient ob/ob mice by 1H NMR spectroscopy.
PLoS One. 2013;8(10):e75998
Authors: Won EY, Yoon MK, Kim SW, Jung Y, Bae HW, Lee D,...
A metabolomic comparison of mouse models of the Neuronal Ceroid Lipofuscinoses
A metabolomic comparison of mouse models of the Neuronal Ceroid Lipofuscinoses
Abstract The Neuronal Ceroid Lipofuscinoses (NCL) are a group of fatal inherited neurodegenerative diseases in humans distinguished by a common clinical pathology, characterized by the accumulation of storage body material in cells and gross brain atrophy. In this study, metabolic changes in three NCL mouse models were examined looking for pathways correlated with neurodegeneration. Two mouse models; motor neuron degeneration (mnd) mouse and a variant model of late infantile NCL, termed the neuronal ceroid...
[NMR paper] High-resolution iterative frequency identification for NMR as a general strategy for multidimensional data collection.
High-resolution iterative frequency identification for NMR as a general strategy for multidimensional data collection.
Related Articles High-resolution iterative frequency identification for NMR as a general strategy for multidimensional data collection.
J Am Chem Soc. 2005 Sep 14;127(36):12528-36
Authors: Eghbalnia HR, Bahrami A, Tonelli M, Hallenga K, Markley JL
We describe a novel approach to the rapid collection and processing of multidimensional NMR data: "high-resolution iterative frequency identification for NMR" (HIFI-NMR). As with...
nmrlearner
Journal club
0
12-01-2010 06:56 PM
(1)H-NMR-based metabolomic study on resistance to diet-induced obesity in AHNAK knock
(1)H-NMR-based metabolomic study on resistance to diet-induced obesity in AHNAK knock-out mice.
(1)H-NMR-based metabolomic study on resistance to diet-induced obesity in AHNAK knock-out mice.
Biochem Biophys Res Commun. 2010 Nov 18;
Authors: Kim IY, Jung J, Jang M, Ahn YG, Shin JH, Choi JW, Sohn MR, Shin SM, Kang DG, Lee HS, Bae YS, Ryu DH, Seong JK, Hwang GS
AHNAK is a giant protein of approximately 700 kD identified in human neuroblastomas and skin epithelial cells. Recently, we found that AHNAK knock-out (AHNAK(-/-)) mice have a strong...
Strategy for NMR metabolomic analysis of urine in mouse models of obesity- from sample collection to interpretation of acquired data.
Linear Mode
