Strategy for complete NMR assignment of disordered proteins with highly repetitive se

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Strategy for complete NMR assignment of disordered proteins with highly repetitive se

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

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Pseudocontact shifts:

Protein geomtery:

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MestReS

V-NMR

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Backbone S2

Methyl S2

B-factor

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Gromacs

Amber

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From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

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MAXOCC

Format conversion & validation:

CCPN

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Validate NMR-STAR 3.1

NMR sample preparation:

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DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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10-05-2010, 12:11 PM

nmrlearner

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Strategy for complete NMR assignment of disordered proteins with highly repetitive se

Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments.

Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments.

J Biomol NMR. 2010 Oct 2;

Authors: MotÃ¡Ä?kovÃ¡ V, NovÃ¡Ä?ek J, Zawadzka-Kazimierczuk A, Kazimierczuk K, ZÃ*dek L, SanderovÃ¡ H, KrÃ¡snÃ½ L, KoÅºmiÅ?ski W, SklenÃ¡Å? V

A strategy for complete backbone and side-chain resonance assignment of disordered proteins with highly repetitive sequence is presented. The protocol is based on three resolution-enhanced NMR experiments: 5D HN(CA)CONH provides sequential connectivity, 5D HabCabCONH is utilized to identify amino acid types, and 5D HC(CC-TOCSY)CONH is used to assign the side-chain resonances. The improved resolution was achieved by a combination of high dimensionality and long evolution times, allowed by non-uniform sampling in the indirect dimensions. Random distribution of the data points and Sparse Multidimensional Fourier Transform processing were used. Successful application of the assignment procedure to a particularly difficult protein, Î´ subunit of RNA polymerase from Bacillus subtilis, is shown to prove the efficiency of the strategy. The studied protein contains a disordered C-terminal region of 81 amino acids with a highly repetitive sequence. While the conventional assignment methods completely failed due to a very small differences in chemical shifts, the presented strategy provided a complete backbone and side-chain assignment.

PMID: 20890634 [PubMed - as supplied by publisher]

Source: PubMed

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