Protein-Specific Discovery Strategies - Genetic Engineering News
Protein-Specific Discovery Strategies - Genetic Engineering News
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Protein-Specific Discovery Strategies
Genetic Engineering News
â??The most challenging aspect is finding ways to express and prepare labeled G-protein-coupled receptors for NMR studies, so that we can learn about the timescales of conformational changes,â?? notes Dr. Kobilka. Structure-function relationship studies ...
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03-17-2014 07:23 PM
[NMR paper] Strategies for Protein NMR in Escherichia coli.
Strategies for Protein NMR in Escherichia coli.
Related Articles Strategies for Protein NMR in Escherichia coli.
Biochemistry. 2014 Mar 5;
Authors: Xu G, Ye Y, Liu X, Cao S, Wu Q, Cheng K, Liu M, Pielak GJ, Li C
Abstract
In-cell NMR spectroscopy provides insight into protein conformation, dynamics and function at atomic resolution in living cells. Systematic evaluation of isotopic labeling strategies is necessary to observe the target protein in the sea of other molecules in the cell. Here, we investigate the detectability, sensitivity and...
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03-08-2014 06:52 AM
[NMR paper] Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
Related Articles Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
J Biomol NMR. 2013 Dec 20;
Authors: Lu GJ, Opella SJ
Abstract
Oriented sample solid-state NMR spectroscopy can be used to determine the three-dimensional structures of membrane proteins in magnetically or mechanically aligned lipid bilayers. The...
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12-21-2013 03:15 PM
Lysine methylation strategies for characterizing protein conformations by NMR
Lysine methylation strategies for characterizing protein conformations by NMR
Abstract In the presence of formaldehyde and a mild reducing agent, reductive methylation is known to achieve near complete dimethylation of protein amino groups under non-denaturing conditions, in aqueous media. Amino methylation of proteins is employed in mass spectrometric, crystallographic, and NMR studies. Where biosynthetic labeling is prohibitive, amino 13C-methylation provides an attractive option for monitoring folding, kinetics, proteinâ??protein and protein-DNA interactions by NMR. Here, we...
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09-10-2012 01:48 AM
Isotope labeling strategies for NMR studies of RNA
Isotope labeling strategies for NMR studies of RNA
Abstract The known biological functions of RNA have expanded in recent years and now include gene regulation, maintenance of sub-cellular structure, and catalysis, in addition to propagation of genetic information. As for proteins, RNA function is tightly correlated with structure. Unlike proteins, structural information for larger, biologically functional RNAs is relatively limited. NMR signal degeneracy, relaxation problems, and a paucity of long-range 1Hâ??1H dipolar contacts have limited the utility of traditional NMR approaches....
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01-09-2011 12:46 PM
[NMR paper] Strategies for the NMR-based identification and optimization of allosteric protein kinase inhibitors.
Strategies for the NMR-based identification and optimization of allosteric protein kinase inhibitors.
Related Articles Strategies for the NMR-based identification and optimization of allosteric protein kinase inhibitors.
Chembiochem. 2005 Sep;6(9):1607-10
Authors: Jahnke W, Blommers MJ, Fernández C, Zwingelstein C, Amstutz R
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12-01-2010 06:56 PM
[NMR paper] Various strategies of using residual dipolar couplings in NMR-driven protein docking: application to Lys48-linked di-ubiquitin and validation against 15N-relaxation data.
Various strategies of using residual dipolar couplings in NMR-driven protein docking: application to Lys48-linked di-ubiquitin and validation against 15N-relaxation data.
Related Articles Various strategies of using residual dipolar couplings in NMR-driven protein docking: application to Lys48-linked di-ubiquitin and validation against 15N-relaxation data.
Proteins. 2005 Aug 15;60(3):367-81
Authors: van Dijk AD, Fushman D, Bonvin AM
When classical, Nuclear Overhauser Effect (NOE)-based approaches fail, it is possible, given high-resolution...
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12-01-2010 06:56 PM
[NMR paper] New developments in isotope labeling strategies for protein solution NMR spectroscopy
New developments in isotope labeling strategies for protein solution NMR spectroscopy.
Related Articles New developments in isotope labeling strategies for protein solution NMR spectroscopy.
Curr Opin Struct Biol. 2000 Oct;10(5):585-92
Authors: Goto NK, Kay LE
The development of novel isotope labeling strategies for proteins has facilitated the study of the structure and dynamics of these molecules. In addition, the recent emergence of alternative methods of bacterial expression for obtaining isotopically labeled proteins permits the study of...
Strategies for Protein NMR in Escherichiacoli
Linear Mode
