Straightforward, effective calibration of SPINAL-64 decoupling results in the enhancement of sensitivity and resolution of biomolecular solid-state NMR.

		BioNMR > Educational resources > Journal club
Straightforward, effective calibration of SPINAL-64 decoupling results in the enhancement of sensitivity and resolution of biomolecular solid-state NMR.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-08-2011, 06:28 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,780

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Straightforward, effective calibration of SPINAL-64 decoupling results in the enhancement of sensitivity and resolution of biomolecular solid-state NMR.

Straightforward, effective calibration of SPINAL-64 decoupling results in the enhancement of sensitivity and resolution of biomolecular solid-state NMR.

Straightforward, effective calibration of SPINAL-64 decoupling results in the enhancement of sensitivity and resolution of biomolecular solid-state NMR.

J Magn Reson. 2010 Dec 31;

Authors: Comellas G, Lopez JJ, Nieuwkoop AJ, Lemkau LR, Rienstra CM

We describe a simple yet highly effective optimization strategy for SPINAL-64 (1)H decoupling conditions for magic-angle spinning solid-state NMR. With adjustment of the phase angles in a coupled manner, the optimal conditions resulting from three parameter optimizations can be determined with adjustment of a single phase. Notably, echo T(2) relaxation times for (13)C and (15)N show significant enhancement (up to 64%), relative to the previous described SPINAL-64 conditions, under the moderate (1)H decoupling levels (60-100kHz) and MAS rate (13.3kHz) commonly employed for high-resolution SSNMR spectroscopy of proteins. Additionally, we also investigated the effect at higher spinning rate (33.3kHz) and compared the results with other (1)H decoupling schemes (TPPM, XiX), as well as SPINAL-64 with the originally reported optimal values.

PMID: 21296014 [PubMed - as supplied by publisher]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Signal enhancement for the sensitivity-limited solid state NMR experiments using a continuous, non-uniform acquisition scheme. Signal enhancement for the sensitivity-limited solid state NMR experiments using a continuous, non-uniform acquisition scheme. Signal enhancement for the sensitivity-limited solid state NMR experiments using a continuous, non-uniform acquisition scheme. J Magn Reson. 2011 Aug 30; Authors: Qiang W Abstract We describe a sampling scheme for the two-dimensional (2D) solid state NMR experiments, which can be readily applied to the sensitivity-limited samples. The sampling scheme utilizes continuous, non-uniform sampling profile for the...	nmrlearner	Journal club	0	09-21-2011 03:31 PM
Solid-State NMR of a Large Membrane Protein by Paramagnetic Relaxation Enhancement. Solid-State NMR of a Large Membrane Protein by Paramagnetic Relaxation Enhancement. Solid-State NMR of a Large Membrane Protein by Paramagnetic Relaxation Enhancement. J Phys Chem Lett. 2011 Jul 21;2(14):1836-1841 Authors: Tang M, Berthold DA, Rienstra CM Membrane proteins play an important role in many biological functions. Solid-state NMR spectroscopy is uniquely suited for studying structure and dynamics of membrane proteins in a membranous environment. The major challenge to obtain high quality solid-state NMR spectra of membrane proteins is...	nmrlearner	Journal club	0	08-16-2011 01:19 PM
(1)H-Detected (13)C Photo-CIDNP as a Sensitivity Enhancement Tool in Solution NMR. (1)H-Detected (13)C Photo-CIDNP as a Sensitivity Enhancement Tool in Solution NMR. (1)H-Detected (13)C Photo-CIDNP as a Sensitivity Enhancement Tool in Solution NMR. J Am Chem Soc. 2011 May 6; Authors: Lee JH, Sekhar A, Cavagnero S NMR is a powerful yet intrinsically insensitive technique. The applicability of NMR to chemical and biological systems would be substantially extended by new approaches going beyond current signal-to-noise capabilities. Here, we exploit the large enhancements arising from (13)C photochemically induced dynamic nuclear...	nmrlearner	Journal club	0	05-10-2011 05:11 PM
1H-Detected 13C Photo-CIDNP as a Sensitivity Enhancement Tool in Solution NMR 1H-Detected 13C Photo-CIDNP as a Sensitivity Enhancement Tool in Solution NMR Jung Ho Lee, Ashok Sekhar and Silvia Cavagnero http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja111613c/aop/images/medium/ja-2010-11613c_0005.gif Journal of the American Chemical Society DOI: 10.1021/ja111613c http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/nDw3hSNxR80	nmrlearner	Journal club	0	05-06-2011 05:57 PM
Straightforward, effective calibration of SPINAL-64 decoupling results in the enhancement of sensitivity and resolution of biomolecular solid-state NMR Straightforward, effective calibration of SPINAL-64 decoupling results in the enhancement of sensitivity and resolution of biomolecular solid-state NMR Publication year: 2010 Source: Journal of Magnetic Resonance, In Press, Accepted Manuscript, Available online 31 December 2010</br> Gemma, Comellas , Jakob J., Lopez , Andrew J., Nieuwkoop , Luisel R., Lemkau , Chad M., Rienstra</br> We describe a simple yet highly effective optimization strategy for SPINAL-64 1H decoupling conditions for magic-angle spinning solid-state NMR. With adjustment of the phase angles in a coupled manner,...	nmrlearner	Journal club	0	01-01-2011 08:57 AM
[NMR paper] Sensitivity enhancement in two-dimensional solid-state NMR spectroscopy by transverse Sensitivity enhancement in two-dimensional solid-state NMR spectroscopy by transverse mixing. Related Articles Sensitivity enhancement in two-dimensional solid-state NMR spectroscopy by transverse mixing. Chemphyschem. 2004 Jun 21;5(6):863-8 Authors: Tycko R The sensitivity of two-dimensional (2D) 13C-13C solid-state NMR spectroscopy under magic-angle spinning (MAS) is shown to be enhanced by the use of transverse polarization transfer in place of the conventional longitudinal polarization transfer. Experimental results are reported for 2D...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] Resolution enhancement in multidimensional solid-state NMR spectroscopy of proteins u Resolution enhancement in multidimensional solid-state NMR spectroscopy of proteins using spin-state selection. Related Articles Resolution enhancement in multidimensional solid-state NMR spectroscopy of proteins using spin-state selection. J Am Chem Soc. 2003 Oct 1;125(39):11816-7 Authors: Duma L, Hediger S, Brutscher B, Böckmann A, Emsley L We show that the resolution of homonuclear multidimensional solid-state NMR correlation experiments can be significantly improved using transition selection and spin-state-selective polarization transfer...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
Solid-state NMR paramagnetic relaxation enhancement immersion depth studies in phosph Solid-state NMR paramagnetic relaxation enhancement immersion depth studies in phospholipid bilayers. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Solid-state NMR paramagnetic relaxation enhancement immersion depth studies in phospholipid bilayers. J Magn Reson. 2010 Aug 24; Authors: Chu S, Maltsev S, Emwas AH, Lorigan GA A new approach for determining the membrane immersion depth of a spin-labeled probe has been developed using paramagnetic relaxation enhancement (PRE)...	nmrlearner	Journal club	0	09-22-2010 05:27 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off