NMR paper STD NMR Epitope Perturbation by Mutation Unveils the Mechanism of YM155 as an Arginine-Glycosyltransferases Inhibitor Effective in Treating Enteropathogenic Diseases

		BioNMR > Educational resources > Journal club
[NMR paper] STD NMR Epitope Perturbation by Mutation Unveils the Mechanism of YM155 as an Arginine-Glycosyltransferases Inhibitor Effective in Treating Enteropathogenic Diseases

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-28-2025, 10:20 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,983

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

STD NMR Epitope Perturbation by Mutation Unveils the Mechanism of YM155 as an Arginine-Glycosyltransferases Inhibitor Effective in Treating Enteropathogenic Diseases

STD NMR Epitope Perturbation by Mutation Unveils the Mechanism of YM155 as an Arginine-Glycosyltransferases Inhibitor Effective in Treating Enteropathogenic Diseases

Enteropathogenic arginine-glycosyltransferases (Arg-GTs) alter higher eukaryotic proteins by attaching a GlcNAc residue to arginine acceptor sites, disrupting essential pathways such as NF-?B signaling, which promotes bacterial survival. These enzymes are potential drug targets for treating related diseases. In this study, we present a novel STD NMR Epitope Perturbation by Mutation spectroscopic approach that, in combination with hydrogen-deuterium exchange mass spectrometry (HDX-MS), and...

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] NMR structure of emfourin, a novel protein metalloprotease inhibitor: insights into the mechanism of action NMR structure of emfourin, a novel protein metalloprotease inhibitor: insights into the mechanism of action Emfourin (M4in) is a protein metalloprotease inhibitor recently discovered in the bacterium Serratia proteamaculans and the prototype of a new family of protein protease inhibitors with an unknown mechanism of action. Protealysin-like proteases (PLPs) of the thermolysin family are natural targets of emfourin-like inhibitors (ELIs) widespread in bacteria and known in archaea. The available data indicate the involvement of PLPs in interbacterial interaction as well as bacterial...	nmrlearner	Journal club	0	03-09-2023 09:10 PM
Protein Discovery Reveals Mechanism of Fatal Prion Brain Diseases - Technology Networks Protein Discovery Reveals Mechanism of Fatal Prion Brain Diseases - Technology Networks Protein Discovery Reveals Mechanism of Fatal Prion Brain Diseases Technology Networks Read here	nmrlearner	Online News	0	07-03-2021 08:12 PM
[ASAP] Comparative Perturbation-Based Modeling of the SARS-CoV-2 Spike Protein Binding with Host Receptor and Neutralizing Antibodies: Structurally Adaptable Allosteric Communication Hotspots Define Spike Sites Targeted by Global Circulating Mutation Comparative Perturbation-Based Modeling of the SARS-CoV-2 Spike Protein Binding with Host Receptor and Neutralizing Antibodies: Structurally Adaptable Allosteric Communication Hotspots Define Spike Sites Targeted by Global Circulating Mutations https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.1c00139/20210426/images/medium/bi1c00139_0013.gif Biochemistry DOI: 10.1021/acs.biochem.1c00139 http://feeds.feedburner.com/~r/acs/bichaw/~4/2WX5bA-38FE More...	nmrlearner	Journal club	0	04-27-2021 10:40 AM
"Ghost" Protein Discovery Reveals Mechanism of Fatal Prion Brain Diseases - Technology Networks "Ghost" Protein Discovery Reveals Mechanism of Fatal Prion Brain Diseases - Technology Networks "Ghost" Protein Discovery Reveals Mechanism of Fatal Prion Brain Diseases Technology Networks Read here	nmrlearner	Online News	0	03-16-2021 06:18 AM
[ASAP] Characterization of the Kinetic Mechanism of Human Protein Arginine Methyltransferase 5 Characterization of the Kinetic Mechanism of Human Protein Arginine Methyltransferase 5 https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00554/20201204-01/images/medium/bi0c00554_0006.gif Biochemistry DOI: 10.1021/acs.biochem.0c00554 http://feeds.feedburner.com/~r/acs/bichaw/~4/CVBS2o_PcdY More...	nmrlearner	Journal club	0	12-05-2020 07:44 AM
Researchers discover potential mechanism to combat haemorrhagic diseases - News-Medical.net http://www.bionmr.com//t3.gstatic.com/images?q=tbn:ANd9GcTyM1uXTIaIw5q1INc3zkifPECmN3ZbTF8KFbDsA_fbY3BMFAuOMi8jvzcjz3gft3z24W0TdB4a News-Medical.net <img alt="" height="1" width="1"> Researchers discover potential mechanism to combat haemorrhagic diseases News-Medical.net "Our group used Nuclear Magnetic Resonance (NMR) spectroscopy studies to investigate the structural properties of an important viral protein required for virulence of the Rift Valley fever virus, a virus that causes infections in both humans and ... and more » Researchers discover potential mechanism to...	nmrlearner	Online News	0	05-11-2015 08:17 AM
Toward the Molecular Basis of Inherited Prion Diseases: NMR Structure of the Human Prion Protein with V210I Mutation. Toward the Molecular Basis of Inherited Prion Diseases: NMR Structure of the Human Prion Protein with V210I Mutation. Toward the Molecular Basis of Inherited Prion Diseases: NMR Structure of the Human Prion Protein with V210I Mutation. J Mol Biol. 2011 Aug 4; Authors: Biljan I, Ilc G, Giachin G, Raspadori A, Zhukov I, Plavec J, Legname G The development of transmissible spongiform encephalopathies (TSEs) is associated with the conversion of the cellular prion protein (PrP(C)) into a misfolded, pathogenic isoform (PrP(Sc)). Spontaneous generation...	nmrlearner	Journal club	0	08-16-2011 01:19 PM
Arginine interactions with anatase TiO(2) (100) surface and the perturbation of (49)T Arginine interactions with anatase TiO(2) (100) surface and the perturbation of (49)Ti NMR chemical shifts - a DFT investigation: relevance to Renu-Seeram bio solar cell. Related Articles Arginine interactions with anatase TiO(2) (100) surface and the perturbation of (49)Ti NMR chemical shifts - a DFT investigation: relevance to Renu-Seeram bio solar cell. J Mol Model. 2010 Sep 21; Authors: Koch R, Lipton AS, Filipek S, Renugopalakrishnan V Density functional theoretical calculations have been utilized to investigate the interaction of the amino...	nmrlearner	Journal club	0	09-22-2010 05:27 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off