Statistical removal of background signals from high-throughput (1)H NMR line-broadening ligand-affinity screens.
J Biomol NMR. 2015 Jul 9;
Authors: Worley B, Sisco NJ, Powers R
Abstract
NMR ligand-affinity screens are vital to drug discovery, are routinely used to screen fragment-based libraries, and used to verify chemical leads from high-throughput assays and virtual screens. NMR ligand-affinity screens are also a highly informative first step towards identifying functional epitopes of unknown proteins, as well as elucidating the biochemical functions of protein-ligand interaction at their binding interfaces. While simple one-dimensional (1)H NMR experiments are capable of indicating binding through a change in ligand line shape, they are plagued by broad, ill-defined background signals from protein (1)H resonances. We present an uncomplicated method for subtraction of protein background in high-throughput ligand-based affinity screens, and show that its performance is maximized when phase-scatter correction is applied prior to subtraction.
PMID: 26156049 [PubMed - as supplied by publisher]
Statistical removal of background signals from high-throughput 1 H NMR line-broadening ligand-affinity screens
Statistical removal of background signals from high-throughput 1 H NMR line-broadening ligand-affinity screens
Abstract
NMR ligand-affinity screens are vital to drug discovery, are routinely used to screen fragment-based libraries, and used to verify chemical leads from high-throughput assays and virtual screens. NMR ligand-affinity screens are also a highly informative first step towards identifying functional epitopes of unknown proteins, as well as elucidating the biochemical functions of proteinā??ligand interaction at their binding interfaces....
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07-08-2015 11:11 PM
[U. of Ottawa NMR Facility Blog] Exponential Line Broadening - Video Tutorial
Exponential Line Broadening - Video Tutorial
Exponential line broadening is an important NMR data processing tool. It involves multiplying the time domain signal by a decaying exponential function prior to Fourier transforming the data into the frequency domain. It is used to improve the signal-to-noise ratio and is more fully described in a previous post. The following short tutorial video demonstrates its use.
http://4.bp.blogspot.com/-CozKHXyqcTA/UUdrJzrbyqI/AAAAAAAABG8/0FLtL9-4af0/s400/lb_vid.jpg
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03-19-2013 12:58 AM
[Question from NMRWiki Q&A forum] residue information in case of line broadening (intensity drop ) of HSQC titration
residue information in case of line broadening (intensity drop ) of HSQC titration
Dear Friends
we have done HSQC gradient titration with targeted proteins , most of cross peaks we observed intensity drop , 30 out of 95 peaks ( 70 percent intensity drop of each cross peak ) , other cross peaks also we observe intensity drop less than 70 percent , we did n"t observe any chemical shift change .( May be it is due to intermediate exchange regime of complex ) .ITC experiment showing 12uM binding constant .AUC experiment result is complex size 33 kd .
is their any method to extract...
nmrlearner
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05-11-2012 10:36 AM
[Question from NMRWiki Q&A forum] How to extract residue information in case of line broadening (intensity drop ) of HSQC titration of protein - protein interaction data ?
How to extract residue information in case of line broadening (intensity drop ) of HSQC titration of protein - protein interaction data ?
Dear Friends
we have done HSQC gradient titration with targeted proteins , most of cross peaks we observed intensity drop , 30 out of 95 peaks ( 70 percent intensity drop of each cross peak ) , other cross peaks also we observe intensity drop less than 70 percent , we did n"t observe any chemical shift change .( May be it is due to intermediate exchange regime of complex ) .ITC experiment showing 12uM binding constant .AUC experiment result is...
nmrlearner
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05-09-2012 07:38 PM
[Question from NMRWiki Q&A forum] Line broadening in nmr
Line broadening in nmr
Hello can some body help me in understanding the line broadening effect in NMR? I am working with protein and small molecules and I observed severe broadening at a protein to ligand ratio 1:20. I am pretty sure that its not aggregation since i have the free ligand spectrum without the protein which didnt showed any broadening effect.
Hope somebody will guide me..
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
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10-04-2011 08:47 PM
[NMR paper] Determination of protein-ligand binding affinity by NMR: observations from serum albu
Determination of protein-ligand binding affinity by NMR: observations from serum albumin model systems.
Related Articles Determination of protein-ligand binding affinity by NMR: observations from serum albumin model systems.
Magn Reson Chem. 2005 Jun;43(6):463-70
Authors: Fielding L, Rutherford S, Fletcher D
The usefulness of bovine serum albumin (BSA) as a model protein for testing NMR methods for the study of protein-ligand interactions is discussed. Isothermal titration calorimetry established the binding affinity and stoichiometry of the...
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11-25-2010 08:21 PM
[NMR paper] Low 13C-background for NMR-based studies of ligand binding using 13C-depleted glucose
Low 13C-background for NMR-based studies of ligand binding using 13C-depleted glucose as carbon source for microbial growth: 13C-labeled glucose and 13C-forskolin binding to the galactose-H+ symport protein GalP in Escherichia coli.
Related Articles Low 13C-background for NMR-based studies of ligand binding using 13C-depleted glucose as carbon source for microbial growth: 13C-labeled glucose and 13C-forskolin binding to the galactose-H+ symport protein GalP in Escherichia coli.
J Am Chem Soc. 2004 Jan 14;126(1):86-7
Authors: Patching SG, Herbert RB,...
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11-24-2010 09:25 PM
[NMR paper] NMR exchange broadening arising from specific low affinity protein self-association:
NMR exchange broadening arising from specific low affinity protein self-association: analysis of nitrogen-15 nuclear relaxation for rat CD2 domain 1.
Related Articles NMR exchange broadening arising from specific low affinity protein self-association: analysis of nitrogen-15 nuclear relaxation for rat CD2 domain 1.
J Biomol NMR. 1999 Aug;14(4):307-20
Authors: Pfuhl M, Chen HA, Kristensen SM, Driscoll PC
Nuclear spin relaxation monitored by heteronuclear NMR provides a useful method to probe the overall and internal molecular motion for...
Statistical removal of background signals from high-throughput (1)H NMR line-broadening ligand-affinity screens.
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