Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis.
J Biomol NMR. 2017 Dec 09;:
Authors: Chen JL, Zhao Y, Gong YJ, Pan BB, Wang X, Su XC
Abstract
Organic synthesis of a ligand with high binding affinities for paramagnetic lanthanide ions is an effective way of generating paramagnetic effects on proteins. These paramagnetic effects manifested in high-resolution NMR spectroscopy are valuable dynamic and structural restraints of proteins and protein-ligand complexes. A paramagnetic tag generally contains a metal chelating moiety and a reactive group for protein modification. Herein we report two new DTPA-like tags, 4PS-PyDTTA and 4PS-6M-PyDTTA that can be site-specifically attached to a protein with a stable thioether bond. Both protein-tag adducts form stable lanthanide complexes, of which the binding affinities and paramagnetic tensors are tunable with respect to the 6-methyl group in pyridine. Paramagnetic relaxation enhancement (PRE) effects of Gd(III) complex on protein-tag adducts were evaluated in comparison with pseudocontact shift (PCS), and the results indicated that both 4PS-PyDTTA and 4PS-6M-PyDTTA tags are rigid and present high-quality PREs that are crucially important in elucidation of the dynamics and interactions of proteins and protein-ligand complexes. We also show that these two tags are suitable for in-situ protein NMR analysis.
PMID: 29224182 [PubMed - as supplied by publisher]
Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis
Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis
Abstract
Organic synthesis of a ligand with high binding affinities for paramagnetic lanthanide ions is an effective way of generating paramagnetic effects on proteins. These paramagnetic effects manifested in high-resolution NMR spectroscopy are valuable dynamic and structural restraints of proteins and proteinâ??ligand complexes. A paramagnetic tag generally contains a metal chelating moiety and a reactive group for protein modification. Herein we...
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12-10-2017 01:21 AM
[NMR paper] Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags.
Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags.
Related Articles Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags.
Prog Nucl Magn Reson Spectrosc. 2017 Feb;98-99:20-49
Authors: Nitsche C, Otting G
PMID: 28283085
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03-12-2017 12:32 PM
Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags
Pseudocontact shifts in biomolecular NMR using paramagnetic metal tags
Publication date: Available online 1 December 2016
Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br>
Author(s): Christoph Nitsche, Gottfried Otting</br>
Graphical abstract
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12-02-2016 07:45 AM
[NMR paper] (1) H-Detected Solid-State NMR Studies of Water-Inaccessible Proteins In Vitro and In Situ.
(1) H-Detected Solid-State NMR Studies of Water-Inaccessible Proteins In Vitro and In Situ.
Related Articles (1) H-Detected Solid-State NMR Studies of Water-Inaccessible Proteins In Vitro and In Situ.
Angew Chem Int Ed Engl. 2016 Sep 27;
Authors: Medeiros-Silva J, Mance D, Daniëls M, Jekhmane S, Houben K, Baldus M, Weingarth M
Abstract
(1) H detection can significantly improve solid-state NMR spectral sensitivity and thereby allows studying more complex proteins. However, the common prerequisite for (1) H detection is the...
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09-28-2016 06:22 PM
[NMR paper] Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis.
Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis.
Related Articles Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis.
J Biomol NMR. 2016 Jan 6;
Authors: Yang Y, Huang F, Huber T, Su XC
Abstract
Design of a paramagnetic metal binding motif in a protein is a valuable way for understanding the function, dynamics and interactions of a protein by paramagnetic...
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01-07-2016 11:10 PM
Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis
Site-specific tagging proteins with a rigid, small and stable transition metal chelator, 8-hydroxyquinoline, for paramagnetic NMR analysis
Abstract
Design of a paramagnetic metal binding motif in a protein is a valuable way for understanding the function, dynamics and interactions of a protein by paramagnetic NMR spectroscopy. Several strategies have been proposed to site-specifically tag proteins with paramagnetic lanthanide ions. Here we report a simple approach of engineering a transition metal binding motif via site-specific labelling of a...
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01-06-2016 09:48 AM
[NMR paper] Installation of a rigid EDTA-like motif into a protein ?-helix for paramagnetic NMR spectroscopy with Co(II) ions.
Installation of a rigid EDTA-like motif into a protein ?-helix for paramagnetic NMR spectroscopy with Co(II) ions.
Installation of a rigid EDTA-like motif into a protein ?-helix for paramagnetic NMR spectroscopy with Co(II) ions.
Chemistry. 2015 Dec 4;
Authors: Swarbrick J, Ung P, Dennis M, Lee M, Chhabra S, Graham B
Abstract
Coupling two copies of an iminodiacetic acid-cysteine hybrid ligand to a pair of cysteine residues positioned in an i, i + 4 arrangement within a protein ?-helix leads to generation of an EDTA-like metal...
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12-05-2015 01:26 PM
Engineering of a bis-chelator motif into a protein ?-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy.
Engineering of a bis-chelator motif into a protein ?-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy.
Engineering of a bis-chelator motif into a protein ?-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy.
Chem Commun (Camb). 2011 May 27;
Authors: Swarbrick JD, Ung P, Su XC, Maleckis A, Chhabra S, Huber T, Otting G, Graham B
Attachment of two nitrilotriacetic acid-based ligands to a protein ?-helix in an i, i + 4 configuration produces an octadentate chelating motif that is able to bind paramagnetic...
Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis.
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