Publication date: Available online 20 June 2016 Source:Progress in Nuclear Magnetic Resonance Spectroscopy
Author(s): Frank H.T. Nelissen, Marco Tessari, Sybren S. Wijmenga, Hans A. Heus
NMR is a powerful method for studying proteins and nucleic acids in solution. The study of nucleic acids by NMR is far more challenging than for proteins, which is mainly due to the limited number of building blocks and unfavorable spectral properties. For NMR studies of DNA molecules, (site specific) isotope enrichment is required to facilitate specific NMR experiments and applications. Here, we provide a comprehensive review of isotope-labeling strategies for obtaining stable isotope labeled DNA as well as specifically stable isotope labeled building blocks required for enzymatic DNA synthesis. Graphical abstract
New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm - Digital Journal
New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm - Digital Journal
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New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm
Digital Journal
Recently, labeling of proteins and peptides with stable heavy isotopes (deuterium, carbon-13, nitrogen-15, and oxygen-18) has been widely used in quantitative proteomics. In fact, such labeling has long been a staple of NMR spectroscopy studies. It can ...
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04-20-2016 04:54 AM
New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm - Press Release Rocket
New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm - Press Release Rocket
<img alt="" height="1" width="1">
New Stable Isotope Labeling from Creative Peptides Accelerates the Development of Protein Quantitative Algorithm
Press Release Rocket
Recently, labeling of proteins and peptides with stable heavy isotopes (deuterium, carbon-13, nitrogen-15, and oxygen-18) has been widely used in quantitative proteomics. In fact, such labeling has long been a staple of NMR spectroscopy studies. It can ...
and more »
Read here
nmrlearner
Online News
0
04-19-2016 04:52 AM
Stable isotope labeling strategy based on coding theory
Stable isotope labeling strategy based on coding theory
Abstract
We describe a strategy for stable isotope-aided protein nuclear magnetic resonance (NMR) analysis, called stable isotope encoding. The basic idea of this strategy is that amino-acid selective labeling can be considered as â??encoding and decodingâ?? processes, in which the information of amino acid type is encoded by the stable isotope labeling ratio of the corresponding residue and it is decoded by analyzing NMR spectra. According to the idea, the strategy can diminish the...
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08-21-2015 09:52 AM
Stable isotope labeling of glycoprotein expressed in silkworms using immunoglobulin G as a test molecule
Stable isotope labeling of glycoprotein expressed in silkworms using immunoglobulin G as a test molecule
Abstract
Silkworms serve as promising bioreactors for the production of recombinant proteins, including glycoproteins and membrane proteins, for structural and functional protein analyses. However, lack of methodology for stable isotope labeling has been a major deterrent to using this expression system for nuclear magnetic resonance (NMR) structural biology. Here we developed a metabolic isotope labeling technique using commercially...
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04-23-2015 04:37 AM
NMR-based stable isotope resolved metabolomics in systems biochemistry
NMR-based stable isotope resolved metabolomics in systems biochemistry
Abstract An important goal of metabolomics is to characterize the changes in metabolic networks in cells or various tissues of an organism in response to external perturbations or pathologies. The profiling of metabolites and their steady state concentrations does not directly provide information regarding the architecture and fluxes through metabolic networks. This requires tracer approaches. NMR is especially powerful as it can be used not only to identify and quantify metabolites in an unfractionated mixture such...
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03-03-2011 02:06 AM
Stable-isotope-assisted NMR approaches to glycoproteins using immunoglobulin G as a m
Stable-isotope-assisted NMR approaches to glycoproteins using immunoglobulin G as a model system.
Related Articles Stable-isotope-assisted NMR approaches to glycoproteins using immunoglobulin G as a model system.
Prog Nucl Magn Reson Spectrosc. 2010 May;56(4):346-59
Authors: Kato K, Yamaguchi Y, Arata Y
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10-19-2010 04:51 PM
[NMR paper] Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for
Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis.
Related Articles Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis.
J Biomol NMR. 1995 Sep;6(2):129-34
Authors: Kigawa T, Muto Y, Yokoyama S
For the application of multidimensional NMR spectroscopy to larger proteins, it would be useful to perform selective labeling of one of the 20 amino acids. For some amino acids, however, amino acid metabolism drastically reduces the efficiency and selectivity...
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08-22-2010 03:50 AM
Stable-isotope-assisted NMR approaches to glycoproteins using immunoglobulin G as a m
Stable-isotope-assisted NMR approaches to glycoproteins using immunoglobulin G as a model system
Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 19 March 2010</br>
Koichi, Kato , Yoshiki, Yamaguchi , Yoji, Arata</br>
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Stable isotope labeling methods for DNA
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