Hyperpolarization via the solid-state photochemically induced dynamic nuclear polarization (photo-CIDNP) effect can be detected in frozen solutions of electron transfer proteins generating a radical-pair upon illumination. The effect has been observed in various natural photosynthetic reaction centers and in light-oxygen-voltage (LOV) sensing domains incorporating a flavin mononucleotide (FMN) as chromophore. In LOV domains, where a highly conserved cysteine is mutated to a flavin to interrupt...
Solid-State Dynamic Nuclear Polarization at 9.4 and 18.8 T from 100 K to Room Temperature
From The DNP-NMR Blog:
Solid-State Dynamic Nuclear Polarization at 9.4 and 18.8 T from 100 K to Room Temperature
Lelli, M., et al., Solid-State Dynamic Nuclear Polarization at 9.4 and 18.8 T from 100 K to Room Temperature. J Am Chem Soc, 2015. 137(46): p. 14558-61.
http://www.ncbi.nlm.nih.gov/pubmed/26555676
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12-07-2015 06:38 PM
Dynamic nuclear polarization of a glassy matrix prepared by solid state mechanochemical amorphization of crystalline substances
From The DNP-NMR Blog:
Dynamic nuclear polarization of a glassy matrix prepared by solid state mechanochemical amorphization of crystalline substances
Elisei, E., et al., Dynamic nuclear polarization of a glassy matrix prepared by solid state mechanochemical amorphization of crystalline substances. Chem Commun (Camb), 2015. 51(11): p. 2080-3.
http://www.ncbi.nlm.nih.gov/pubmed/25534192
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04-09-2015 03:47 AM
Dynamic nuclear polarization of a glassy matrix prepared by solid state mechanochemical amorphization of crystalline substances
From The DNP-NMR Blog:
Dynamic nuclear polarization of a glassy matrix prepared by solid state mechanochemical amorphization of crystalline substances
This is a very interesting article describing the preparation of a sample for DNP by co-milling the analyte with the polarizing substrate. For a long time it was thought that just mechanically mixing the polarizing agent with the analyte will not result in a sample that is useful for DNP because the radical has to be mixed at an atomic level. Clearly this article demonstrate that this is not necessary. This will open up DNP to be used...
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02-09-2015 07:49 PM
Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments
From The DNP-NMR Blog:
Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments
Takahashi, H., et al., Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments. J Magn Reson, 2013. 239C(0): p. 91-99.
http://www.ncbi.nlm.nih.gov/pubmed/24480716
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02-10-2014 08:46 PM
Proton polarization in photo-excited aromatic molecule at room temperature enhanced by intense optical source and temperature control
From The DNP-NMR Blog:
Proton polarization in photo-excited aromatic molecule at room temperature enhanced by intense optical source and temperature control
Sakaguchi, S., et al., Proton polarization in photo-excited aromatic molecule at room temperature enhanced by intense optical source and temperature control. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2013. 317(0): p. 679-684.
http://www.sciencedirect.com/science/article/pii/S0168583X13008872
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01-23-2014 01:37 AM
Proton polarization in photo-excited aromatic molecule at room temperature enhanced by intense optical source and temperature control
From The DNP-NMR Blog:
Proton polarization in photo-excited aromatic molecule at room temperature enhanced by intense optical source and temperature control
Sakaguchi, S., et al., Proton polarization in photo-excited aromatic molecule at room temperature enhanced by intense optical source and temperature control. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 2013(0).
http://www.sciencedirect.com/science/article/pii/S0168583X13008872
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11-21-2013 01:14 AM
Bacteriopheophytin a in the active branch of the reaction center of rhodobacter sphaeroides is not disturbed by the protein matrix as shown by 13C photo-CIDNP MAS NMR
From The DNP-NMR Blog:
Bacteriopheophytin a in the active branch of the reaction center of rhodobacter sphaeroides is not disturbed by the protein matrix as shown by 13C photo-CIDNP MAS NMR
Sai Sankar Gupta, K.B., et al., Bacteriopheophytin a in the Active Branch of the Reaction Center of Rhodobacter sphaeroides Is Not Disturbed by the Protein Matrix as Shown by 13C Photo-CIDNP MAS NMR. The Journal of Physical Chemistry B, 2013. 117(12): p. 3287-3297.
http://www.ncbi.nlm.nih.gov/pubmed/23452037
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06-22-2013 01:40 AM
[NMR paper] Bacteriopheophytin a in the Active Branch of the Reaction Center of Rhodobacter Sphaeroides is Not Disturbed by the Protein Matrix as Shown by 13C Photo-CIDNP MAS NMR.
Bacteriopheophytin a in the Active Branch of the Reaction Center of Rhodobacter Sphaeroides is Not Disturbed by the Protein Matrix as Shown by 13C Photo-CIDNP MAS NMR.
Bacteriopheophytin a in the Active Branch of the Reaction Center of Rhodobacter Sphaeroides is Not Disturbed by the Protein Matrix as Shown by 13C Photo-CIDNP MAS NMR.
J Phys Chem B. 2013 Mar 1;
Authors: Sai Sankar Gupta KB, Alia A, Buda F, de Groot HJ, Matysik J
Abstract
The electronic structure of bacteriopheophytin a (BPhe a), the primary electron acceptor (?A) in...
Stabilization of a flavoprotein for solid-state photo-CIDNP MAS NMR at room temperature by embedding in a glassy sugar matrix
Linear Mode
