Publication date: April 2015 Source:Journal of Magnetic Resonance, Volume 253
Author(s): Anja Böckmann , Matthias Ernst , Beat H. Meier
Magic-angle spinning (MAS) is a technique that is a prerequisite for high-resolution solid-state NMR spectroscopy of proteins and other biomolecules. Recently, the 100kHz limit for the rotation frequency has been broken, arguably making MAS rotors the man-made objects with the highest rotation frequency. This development is expected to have a significant impact on biomolecular NMR as it facilitates proton detection, which allows to partially compensate the loss in overall sensitivity associated with the small sample amounts that fit into MAS rotors with less than 1mm outer diameter. Under these conditions, the mass-normalized sensitivity of a small rotor becomes much higher than that of larger-volume rotor. Graphical abstract
Recent advances in magic angle spinning solid state NMR of membrane proteins
From The DNP-NMR Blog:
Recent advances in magic angle spinning solid state NMR of membrane proteins
Wang, S. and V. Ladizhansky, Recent advances in magic angle spinning solid state NMR of membrane proteins. Prog. NMR. Spec., 2014. 82(0): p. 1-26.
http://www.sciencedirect.com/science/article/pii/S0079656514000478
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08-29-2014 05:36 PM
[NMR paper] Rapid proton-detected NMR assignment for proteins with fast magic angle spinning.
Rapid proton-detected NMR assignment for proteins with fast magic angle spinning.
Rapid proton-detected NMR assignment for proteins with fast magic angle spinning.
J Am Chem Soc. 2014 Aug 7;
Authors: Barbet-Massin E, Pell AJ, Retel J, Andreas LB, Jaudzems K, Franks WT, Nieuwkoop AJ, Hiller M, Higman VA, Guerry P, Bertarello A, Knight MJ, Felletti M, Le Marchand T, Kotelovica S, Akopjana I, Tars K, Stoppini M, Bellotti V, Bolognesi M, Ricagno S, Chou JJ, Griffin RG, Oschkinat H, Lesage A, Emsley L, Herrmann T, Pintacuda G
Abstract
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08-08-2014 01:45 PM
Recent advances in magic angle spinning solid state NMR of membrane proteins
Recent advances in magic angle spinning solid state NMR of membrane proteins
Publication date: Available online 26 July 2014
Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br>
Author(s): Shenlin Wang , Vladimir Ladizhansky</br>
Membrane proteins mediate many critical functions in cells. Determining their three-dimensional structures in the native lipid environment has been one of the main objectives in structural biology. There are two major NMR methodologies that allow this objective to be accomplished. Oriented sample NMR, which can be applied to...
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07-27-2014 01:05 AM
[NMR paper] Solid-state NMR Spectra of lipid-anchored Proteins under Magic Angle Spinning.
Solid-state NMR Spectra of lipid-anchored Proteins under Magic Angle Spinning.
Related Articles Solid-state NMR Spectra of lipid-anchored Proteins under Magic Angle Spinning.
J Phys Chem B. 2014 Feb 11;
Authors: Nomura K, Harada E, Sugase K, Shimamoto K
Abstract
Solid-state NMR is a promising tool for elucidating membrane-related biological phenomena. We achieved the measurement of high-resolution solid-state NMR spectra for a lipid-anchored protein embedded in lipid bilayers under magic angle spinning (MAS). To date, solid-state NMR...
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02-13-2014 03:35 PM
[NMR paper] Quadruple-Resonance Magic-Angle Spinning NMR Spectroscopy of Deuterated Solid Proteins.
Quadruple-Resonance Magic-Angle Spinning NMR Spectroscopy of Deuterated Solid Proteins.
Related Articles Quadruple-Resonance Magic-Angle Spinning NMR Spectroscopy of Deuterated Solid Proteins.
Angew Chem Int Ed Engl. 2014 Jan 29;
Authors: Akbey U, Nieuwkoop AJ, Wegner S, Voreck A, Kunert B, Bandara P, Engelke F, Nielsen NC, Oschkinat H
Abstract
(1) H-detected magic-angle spinning NMR experiments facilitate structural biology of solid proteins, which requires using deuterated proteins. However, often amide protons cannot be back-exchanged...
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01-30-2014 05:38 PM
[NMR paper] Magic Angle Spinning NMR Structure Determination of Proteins from Pseudocontact Shifts.
Magic Angle Spinning NMR Structure Determination of Proteins from Pseudocontact Shifts.
Related Articles Magic Angle Spinning NMR Structure Determination of Proteins from Pseudocontact Shifts.
J Am Chem Soc. 2013 May 6;
Authors: Li J, Pilla KB, Li Q, Zhang Z, Su X, Huber T, Yang J
Abstract
Magic angle spinning solid-state NMR is a unique technique to study atomic-resolution structure of biomacromolecules which resist crystallization or are too large to study by solution NMR techniques. However, difficulties in obtaining sufficient number...
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05-08-2013 02:49 PM
[NMR paper] Magic Angle Spinning NMR of Paramagnetic Proteins.
Magic Angle Spinning NMR of Paramagnetic Proteins.
Related Articles Magic Angle Spinning NMR of Paramagnetic Proteins.
Acc Chem Res. 2013 Mar 18;
Authors: Knight MJ, Felli IC, Pierattelli R, Emsley L, Pintacuda G
Abstract
Metal ions are ubiquitous in biochemical and cellular processes. Since many metal ions are paramagnetic due to the presence of unpaired electrons, paramagnetic molecules are an important class of targets for research in structural biology and related fields. Today, NMR spectroscopy plays a central role in the...
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03-20-2013 01:47 PM
[NMR software blog] Faster Faster Faster
Faster Faster Faster
Our machines, even when hitting an apparent performance peak, only run at one small fraction of their true potential speed. I feel that today's computers and their software are OK for routine spectra. I couldn't ask for more. Other spectra are quite large, however, and I must wait a few seconds during processing. Without going into the third dimension, consider these novel experiments to measure long range heteronuclear Js. Each row contains at least 4096 points. Quite likely we are going to see larger rows in the next few years. The time required to compute the FFT is...
Spinning proteins, the faster, the better?
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