Protein motions over various time scales are crucial for protein function. NMR relaxation dispersion experiments play a key role in explaining these motions. However, the study of slow conformational changes with lowly populated states remained elusive. The recently developed exchange-mediated saturation transfer experiments allow the detection and characterization of such motions, but require extensive measurement time. Here we show that, by making use of Fourier transform, the total acquisition time required to measure an exchange-mediated saturation transfer profile can be reduced by twofold in case that one applies linear prediction. In addition, we demonstrate that the analytical solution for R1Ï? experiments can be used for fitting the exchange-mediated saturation transfer profile. Furthermore, we show that simultaneous analysis of exchange-mediated saturation transfer profiles with two different radio-frequency field strengths is required for accurate and precise characterization of the exchange process and the exchanging states.
[NMR paper] Comparison of the sensitivities of WaterLOGSY and saturation transfer difference NMR experiments.
Comparison of the sensitivities of WaterLOGSY and saturation transfer difference NMR experiments.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Comparison of the sensitivities of WaterLOGSY and saturation transfer difference NMR experiments.
J Biomol NMR. 2014 Sep;60(1):37-44
Authors: Antanasijevic A, Ramirez B, Caffrey M
Abstract
The WaterLOGSY (WL) and saturation transfer difference (STD) NMR experiments have proven...
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05-13-2015 02:01 PM
Measuring hydrogen exchange in proteins by selective water saturation in 1Hâ??15N SOFAST/BEST-type experiments: advantages and limitations
Measuring hydrogen exchange in proteins by selective water saturation in 1Hâ??15N SOFAST/BEST-type experiments: advantages and limitations
Abstract
HETex-SOFAST NMR (Schanda et al. in J Biomol NMR 33:199â??211, 2006) has been proposed some years ago as a fast and sensitive method for semi-quantitative measurement of site-specific amide-water hydrogen exchange effects along the backbone of proteins. Here we extend this concept to BEST readout sequences that provide a better resolution at the expense of some loss in sensitivity. We discuss the...
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08-30-2014 11:00 PM
Comparison of the sensitivities of WaterLOGSY and saturation transfer difference NMR experiments
Comparison of the sensitivities of WaterLOGSY and saturation transfer difference NMR experiments
Abstract
The WaterLOGSY (WL) and saturation transfer difference (STD) NMR experiments have proven to be extremely useful techniques to characterize interactions between small molecules and large biomolecules. In this work we compare the relative sensitivities of WL and STD NMR using 3 experimental systems: ketoprofen (KET)â??bovine serum albumin (BSA), tert-butyl hydroquinone (TBHQ)â??hemagglutinin (HA), and chloramphenicol (CAM)â??ribosome (70S). In all...
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07-12-2014 06:07 PM
[NMR paper] Probing Slow Chemical Exchange at Carbonyl Sites in Proteins by Chemical Exchange Saturation Transfer NMR Spectroscopy.
Probing Slow Chemical Exchange at Carbonyl Sites in Proteins by Chemical Exchange Saturation Transfer NMR Spectroscopy.
Probing Slow Chemical Exchange at Carbonyl Sites in Proteins by Chemical Exchange Saturation Transfer NMR Spectroscopy.
Angew Chem Int Ed Engl. 2013 Feb 28;
Authors: Vallurupalli P, Kay LE
Abstract
Seeing the invisible: A 13 CO NMR chemical exchange saturation transfer (CEST) experiment for the study of "invisible" excited protein states with lifetimes on the order of 5-50 ms has been developed. The 13 CO chemical...
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03-02-2013 11:45 AM
[CNS Yahoo group] Re: Fourier Transform in CNS
Re: Fourier Transform in CNS
Please read my paper in Acta Cryst A which describes the method implemented in both X-PLOR and CNS. A. T. Brunger, A memory-efficient fast Fourier
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05-31-2012 03:59 AM
[CNS Yahoo group] Fourier Transform in CNS
Fourier Transform in CNS
Hi, I was wandering how the Fourier transform function is defined in CNS. When 'ft' is used, is it a normal fast-fourier-transform call or is there more to it?
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05-31-2012 03:59 AM
[U. of Ottawa NMR Facility Blog] Saturation Transfer and Exchange
Saturation Transfer and Exchange
Exchange processes that occur on the NMR time scale affect the NMR line shapes and can be studied by line shape analysis. If the exchange process is slow on the NMR time scale, one can employ EXSY or inversion transfer methods to study the exchange. An alternative to these is the saturation transfer technique. In this method, one of the slowly exchanging resonances (A) is saturated with low power CW irradiation and the effect on the intensity of the resonance of the exchange partner (B) is monitored. If there is exchange between A and B during the period of...
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08-03-2011 01:00 AM
[NMR paper] Saturation transfer difference (STD) 1H-NMR experiments and in silico docking experim
Saturation transfer difference (STD) 1H-NMR experiments and in silico docking experiments to probe the binding of N-acetylneuraminic acid and derivatives to Vibrio cholerae sialidase.
Related Articles Saturation transfer difference (STD) 1H-NMR experiments and in silico docking experiments to probe the binding of N-acetylneuraminic acid and derivatives to Vibrio cholerae sialidase.
Proteins. 2004 Aug 1;56(2):346-53
Authors: Haselhorst T, Wilson JC, Thomson RJ, McAtamney S, Menting JG, Coppel RL, von Itzstein M
Saturation transfer difference...
Speeding-up exchange-mediated saturation transfer experiments by Fourier transform
Linear Mode
