The protein ?-syn adopts a wide variety of conformations including an intrinsically disordered monomeric form and an ?-helical rich membrane-associated form that is thought to play an important role in cellular membrane processes. However, despite the high affinity of ?-syn for membranes, evidence that the ?-helical form of ?-syn is adopted inside cells has thus far been indirect. In cell DNP-assisted solid state NMR on frozen samples has the potential to report directly on the entire...
[NMR paper] Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR
Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR
Liquid-Liquid phase separation has emerged as fundamental process underlying the formation of biomolecular condensates. Insights into the composition and structure of biomolecular condensates is, however, complicated by their molecular complexity and dynamics. Here, we introduce an improved spatially-resolved NMR experiment that enables quantitative analysis of the physico-chemical composition of multi-component biomolecular condensates in equilibrium and label-free. Application of...
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02-28-2023 05:29 AM
[ASAP] In-Cell Sensitivity-Enhanced NMR of Intact Viable Mammalian Cells
In-Cell Sensitivity-Enhanced NMR of Intact Viable Mammalian Cells
Rupam Ghosh, Yiling Xiao, Jaka Kragelj, and Kendra K. Frederick
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.1c06680/20211101/images/medium/ja1c06680_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.1c06680
http://feeds.feedburner.com/~r/acs/jacsat/~4/Px8tejOwLAA
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11-02-2021 07:28 AM
[NMR paper] In-Cell Sensitivity-Enhanced NMR of Intact Viable Mammalian Cells
In-Cell Sensitivity-Enhanced NMR of Intact Viable Mammalian Cells
NMR has the resolution and specificity to determine atomic-level protein structures of isotopically labeled proteins in complex environments, and with the sensitivity gains conferred by dynamic nuclear polarization (DNP), NMR has the sensitivity to detect proteins at their endogenous concentrations. However, DNP sensitivity enhancements are critically dependent on experimental conditions and sample composition. While some of these conditions are theoretically compatible with cellular viability,...
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11-02-2021 07:28 AM
Spatially resolved NMR spectroscopy of heterogeneous gas phase hydrogenation of 1,3-butadiene with para-hydrogen
From The DNP-NMR Blog:
Spatially resolved NMR spectroscopy of heterogeneous gas phase hydrogenation of 1,3-butadiene with para-hydrogen
Svyatova, Alexandra, Elizaveta S. Kononenko, Kirill V. Kovtunov, Dmitry Lebedev, Evgeniy Yu. Gerasimov, Andrey V. Bukhtiyarov, Igor P. Prosvirin, et al. “Spatially Resolved NMR Spectroscopy of Heterogeneous Gas Phase Hydrogenation of 1,3-Butadiene with Para-Hydrogen.” Catalysis Science & Technology 10, no. 1 (2020): 99–104.
https://doi.org/10.1039/C9CY02100K.
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08-17-2020 11:58 PM
Spatially resolved NMR spectroscopy of heterogeneous gas phase hydrogenation of 1,3-butadiene with para-hydrogen
From The DNP-NMR Blog:
Spatially resolved NMR spectroscopy of heterogeneous gas phase hydrogenation of 1,3-butadiene with para-hydrogen
Svyatova, Alexandra, Elizaveta S. Kononenko, Kirill V. Kovtunov, Dmitry Lebedev, Evgeniy Yu. Gerasimov, Andrey V. Bukhtiyarov, Igor P. Prosvirin, et al. “Spatially Resolved NMR Spectroscopy of Heterogeneous Gas Phase Hydrogenation of 1,3-Butadiene with Para Hydrogen.” Catalysis Science & Technology 10, no. 1 (2020): 99–104
https://doi.org/10.1039/C9CY02100K.
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08-07-2020 08:10 PM
Journal Highlight: Mutual diffusion driven NMR: a new approach for the analysis of mixtures by spatially resolved NMR spectroscopy
Journal Highlight: Mutual diffusion driven NMR: a new approach for the analysis of mixtures by spatially resolved NMR spectroscopy
http://www.spectroscopynow.com/common/images/thumbnails/15c4eade5e6.jpgA new approach has been established for resolving the NMR spectra of mixtures, relying on the mutual diffusion of dissolved species when a concentration gradient is established within the NMR tube.
Read the rest at Spectroscopynow.com
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05-29-2017 09:59 AM
[NMR paper] Mapping long-range interactions in alpha-synuclein using spin-label NMR and ensemble
Mapping long-range interactions in alpha-synuclein using spin-label NMR and ensemble molecular dynamics simulations.
Related Articles Mapping long-range interactions in alpha-synuclein using spin-label NMR and ensemble molecular dynamics simulations.
J Am Chem Soc. 2005 Jan 19;127(2):476-7
Authors: Dedmon MM, Lindorff-Larsen K, Christodoulou J, Vendruscolo M, Dobson CM
The intrinsically disordered protein alpha-synuclein plays a key role in the pathogenesis of Parkinson's disease (PD). We show here that the native state of alpha-synuclein...
Spatially resolved DNP-assisted NMR illuminates the conformational ensemble of ?-synuclein in intact viable cells
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