[NMR paper] New Views of Functionally Dynamic Proteins by Solution NMR Spectroscopy.
New Views of Functionally Dynamic Proteins by Solution NMR Spectroscopy.
Related Articles New Views of Functionally Dynamic Proteins by Solution NMR Spectroscopy.
J Mol Biol. 2015 Dec 18;
Authors: Kay LE
Abstract
In the past several decades solution NMR spectroscopy has emerged as a powerful technique for the study of the structure and dynamics of proteins, providing detailed insights into biomolecular function. Herein I provide a summary of two important areas of application, focusing on NMR studies of (i) supra-molecular...
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12-29-2015 08:05 PM
New Views of Functionally Dynamic Proteins by Solution NMR Spectroscopy
New Views of Functionally Dynamic Proteins by Solution NMR Spectroscopy
Publication date: Available online 19 December 2015
Source:Journal of Molecular Biology</br>
Author(s): Lewis E. Kay</br>
In the past several decades solution NMR spectroscopy has emerged as a powerful technique for the study of the structure and dynamics of proteins, providing detailed insights into biomolecular function. Herein I provide a summary of two important areas of application, focusing on NMR studies of (i) supra-molecular systems with aggregate molecular masses in the hundreds of...
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12-28-2015 12:26 AM
[NMR paper] Solution NMR Spectroscopy Provides an Avenue for the Study of Functionally Dynamic Molecular Machines: The Example of Protein Disaggregation.
Solution NMR Spectroscopy Provides an Avenue for the Study of Functionally Dynamic Molecular Machines: The Example of Protein Disaggregation.
Solution NMR Spectroscopy Provides an Avenue for the Study of Functionally Dynamic Molecular Machines: The Example of Protein Disaggregation.
J Am Chem Soc. 2015 Dec 11;
Authors: Rosenzweig R, Kay LE
Abstract
Solution-based NMR spectroscopy has been an important tool for studying the structure and dynamics of relatively small proteins and protein complexes with aggregate molecular masses...
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12-15-2015 08:09 PM
[NMR paper] (13)CHD 2-CEST NMR spectroscopy provides an avenue for studies of conformational exchange in high molecular weight proteins.
(13)CHD 2-CEST NMR spectroscopy provides an avenue for studies of conformational exchange in high molecular weight proteins.
Related Articles (13)CHD 2-CEST NMR spectroscopy provides an avenue for studies of conformational exchange in high molecular weight proteins.
J Biomol NMR. 2015 Aug 14;
Authors: Rennella E, Huang R, Velyvis A, Kay LE
Abstract
An NMR experiment for quantifying slow (millisecond) time-scale exchange processes involving the interconversion between visible ground state and invisible, conformationally excited...
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08-15-2015 04:01 PM
13 CHD 2 â??CEST NMR spectroscopy provides an avenue for studies of conformational exchange in high molecular weight proteins
13 CHD 2 â??CEST NMR spectroscopy provides an avenue for studies of conformational exchange in high molecular weight proteins
Abstract
An NMR experiment for quantifying slow (millisecond) time-scale exchange processes involving the interconversion between visible ground state and invisible, conformationally excited state conformers is presented. The approach exploits chemical exchange saturation transfer (CEST) and makes use of 13CHD2 methyl group probes that can be readily incorporated into otherwise highly deuterated proteins. The methodology is...
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08-14-2015 10:12 PM
[NMR paper] Bringing Dynamic Molecular Machines into Focus by Methyl-TROSY NMR.
Bringing Dynamic Molecular Machines into Focus by Methyl-TROSY NMR.
Related Articles Bringing Dynamic Molecular Machines into Focus by Methyl-TROSY NMR.
Annu Rev Biochem. 2014 Jun 2;83:291-315
Authors: Rosenzweig R, Kay LE
Abstract
Large macromolecular assemblies, so-called molecular machines, are critical to ensuring proper cellular function. Understanding how proper function is achieved at the atomic level is crucial to advancing multiple avenues of biomedical research. Biophysical studies often include X-ray diffraction and...
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06-07-2014 07:12 PM
Big Data Tackles Tiny Molecular Machines - Science 2.0
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Big Data Tackles Tiny Molecular Machines
Science 2.0
"Structural techniques like X-ray crystallography and nuclear magnetic resonance have worked quite well to help us understand how smaller proteins function," Onuchic said. X-rays only take snapshots of constantly moving proteins, he said, "but ...
Big Data Tackles Tiny Molecular Machines - Science 2.0
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03-16-2014 02:03 AM
[NMR paper] Structural Investigations of Molecular Machines by Solid-State NMR.
Structural Investigations of Molecular Machines by Solid-State NMR.
Related Articles Structural Investigations of Molecular Machines by Solid-State NMR.
Acc Chem Res. 2013 Mar 15;
Authors: Loquet A, Habenstein B, Lange A
Abstract
Essential biological processes such as cell motion, signaling,protein synthesis, and pathogen-host interactions rely on multifunctional molecular machines containing supramolecular assemblies, that is, noncovalently assembled protein subunits. Scientists would like to acquire a detailed atomic view of the complete...
SolutionNMR Spectroscopy Provides an Avenue for theStudy of Functionally Dynamic Molecular Machines: The Example of ProteinDisaggregation
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