Solution-state methyl NMR spectroscopy of large non-deuterated proteins enabled by deep neural networks
 

Solution-state methyl NMR spectroscopy of large non-deuterated proteins enabled by deep neural networks

Methyl-TROSY nuclear magnetic resonance (NMR) spectroscopy is a powerful technique for characterising large biomolecules in solution. However, preparing samples for these experiments is demanding and entails deuteration, limiting its use. Here we demonstrate that NMR spectra recorded on protonated, uniformly ^(13)C labelled samples can be processed using deep neural networks to yield spectra that are of similar quality to typical deuterated methyl-TROSY spectra, potentially providing information...

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