Nuclear magnetic resonance (NMR) spectroscopy is well-established nowadays for the elucidation of the 3D structures of proteins and protein complexes, the evaluation of biomolecular dynamics with atomistic resolution across a range of time scales, the screening of drug candidates with site specificity, and for the quantitation of molecular translational diffusion. Lyotropic lipidic cubic phases (LCPs) are lipid bilayer-based materials with a complex geometry, formed via the spontaneous...
[NMR paper] NMR spectroscopy of lipidic cubic phases
NMR spectroscopy of lipidic cubic phases
Lipidic cubic phase (LCP) structures have been used for stabilisation and crystallisation of membrane proteins and show promising properties as drug carriers. In this mini-review, we present how NMR spectroscopy has played a major role in understanding the physico-chemical properties of LCPs and how recent advances in pulsed field gradient NMR techniques open new perspectives in characterising encapsulated molecules.
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03-28-2022 07:15 PM
[NMR paper] Physiochemical Characterization and Stability of Lipidic Cubic Phases by Solution NMR.
Physiochemical Characterization and Stability of Lipidic Cubic Phases by Solution NMR.
Related Articles Physiochemical Characterization and Stability of Lipidic Cubic Phases by Solution NMR.
Langmuir. 2020 May 16;:
Authors: Meikle T, Keizer DW, Babon JJ, Drummond CJ, Separovic F, Conn CE, Yao S
Abstract
Lipidic inverse bicontinuous cubic phases (LCPs), formed via the spontaneous self-assembly of lipids such as monoolein, have found increasing applications in the stabilization and crystallization of integral membrane proteins for...
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05-19-2020 09:51 PM
[NMR paper] Distinguishing Bicontinuous Lipid Cubic Phases from Isotropic Membrane Morphologies Using (31)P Solid-State NMR Spectroscopy.
Distinguishing Bicontinuous Lipid Cubic Phases from Isotropic Membrane Morphologies Using (31)P Solid-State NMR Spectroscopy.
Distinguishing Bicontinuous Lipid Cubic Phases from Isotropic Membrane Morphologies Using (31)P Solid-State NMR Spectroscopy.
J Phys Chem B. 2015 Mar 27;
Authors: Yang Y, Yao H, Hong M
Abstract
Nonlamellar lipid membranes are frequently induced by proteins that fuse, bend, and cut membranes. Understanding the mechanism of action of these proteins requires the elucidation of the membrane morphologies...
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03-31-2015 07:17 PM
A solution NMR view of protein dynamics in the biological membrane.
A solution NMR view of protein dynamics in the biological membrane.
A solution NMR view of protein dynamics in the biological membrane.
Curr Opin Struct Biol. 2011 Jul 30;
Authors: Chill JH, Naider F
Structure determination of membrane-associated proteins (MPs) represents a frontier of structural biology that is characterized by unique challenges in sample preparation and data acquisition. No less important is our ability to study the dynamics of MPs, since MP flexibility and characteristic motions often make sizeable contributions to their...
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08-03-2011 12:00 PM
Protein dynamics and allostery: an NMR view.
Protein dynamics and allostery: an NMR view.
Related Articles Protein dynamics and allostery: an NMR view.
Curr Opin Struct Biol. 2010 Nov 23;
Authors: Tzeng SR, Kalodimos CG
Allostery, the process by which distant sites within a protein system are energetically coupled, is an efficient and ubiquitous mechanism for activity regulation. A purely mechanical view of allostery invoking only structural changes has developed over the decades as the classical view of the phenomenon. However, a fast growing list of examples illustrate the intimate link...
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11-27-2010 02:45 PM
[NMR paper] Solution structure and dynamics of ras p21.GDP determined by heteronuclear three- and
Solution structure and dynamics of ras p21.GDP determined by heteronuclear three- and four-dimensional NMR spectroscopy.
Related Articles Solution structure and dynamics of ras p21.GDP determined by heteronuclear three- and four-dimensional NMR spectroscopy.
Biochemistry. 1994 Mar 29;33(12):3515-31
Authors: Kraulis PJ, Domaille PJ, Campbell-Burk SL, Van Aken T, Laue ED
A high-resolution solution structure of the GDP form of a truncated version of the ras p21 protein (residues 1-166) has been determined using NMR spectroscopy. Ras p21 is the...
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08-22-2010 03:33 AM
[NMR paper] Solution structure and dynamics of ras p21.GDP determined by heteronuclear three- and
Solution structure and dynamics of ras p21.GDP determined by heteronuclear three- and four-dimensional NMR spectroscopy.
Related Articles Solution structure and dynamics of ras p21.GDP determined by heteronuclear three- and four-dimensional NMR spectroscopy.
Biochemistry. 1994 Mar 29;33(12):3515-31
Authors: Kraulis PJ, Domaille PJ, Campbell-Burk SL, Van Aken T, Laue ED
A high-resolution solution structure of the GDP form of a truncated version of the ras p21 protein (residues 1-166) has been determined using NMR spectroscopy. Ras p21 is the...
A solution NMR view of lipidic cubic phases: Structure, dynamics, and beyond
Linear Mode
