Solution NMR studies of polytopic ?-helical membrane proteins.

		BioNMR > Educational resources > Journal club
Solution NMR studies of polytopic ?-helical membrane proteins.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-23-2011, 08:54 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Solution NMR studies of polytopic ?-helical membrane proteins.

Solution NMR studies of polytopic ?-helical membrane proteins.

Solution NMR studies of polytopic ?-helical membrane proteins.

Curr Opin Struct Biol. 2011 Jul 18;

Authors: Nietlispach D, Gautier A

NMR spectroscopy has established itself as one of the main techniques for the structural study of integral membrane proteins. Remarkably, over the last few years, substantial progress has been achieved in the structure determination of increasingly complex polytopical ?-helical membrane proteins, with their size approaching ~100kDa. Such advances are the result of significant improvements in NMR methodology, sample preparation and powerful selective isotope labelling schemes. We review the requirements facilitating such work based on the more recent solution NMR studies of ?-helical proteins. While the majority of such studies still use detergent-solubilized proteins, alternative more native-like lipid-based media are emerging. Recent interaction, dynamics and conformational studies are discussed that cast a promising light on the future role of NMR in this important and exciting area.

PMID: 21775128 [PubMed - as supplied by publisher]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Solution NMR Approaches for Establishing Specificity of Weak Heterodimerization of Membrane Proteins Solution NMR Approaches for Establishing Specificity of Weak Heterodimerization of Membrane Proteins Tiandi Zhuang, Bing K. Jap and Charles R. Sanders http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja208972h/aop/images/medium/ja-2011-08972h_0009.gif Journal of the American Chemical Society DOI: 10.1021/ja208972h http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/J2oj2lBVCo4	nmrlearner	Journal club	0	11-30-2011 10:45 PM
NMR structures of polytopic integral membrane proteins. NMR structures of polytopic integral membrane proteins. NMR structures of polytopic integral membrane proteins. Mol Membr Biol. 2011 Aug 2; Authors: Patching SG Abstract Membrane proteins represent up to 30% of the proteins in all organisms, they are involved in many biological processes and are the molecular targets for around 50% of validated drugs. Despite this, membrane proteins represent less than 1% of all high-resolution protein structures due to various challenges associated with applying the main biophysical techniques used for...	nmrlearner	Journal club	0	08-04-2011 11:41 AM
Solution NMR study of integral membrane proteins. Solution NMR study of integral membrane proteins. Solution NMR study of integral membrane proteins. Curr Opin Chem Biol. 2011 Jun 18; Authors: Kang C, Li Q Signals between a cell and its environment are often transmitted through membrane proteins; therefore, many membrane proteins, including G protein-coupled receptors (GPCRs) and ion channels, are important drug targets. Structural information about membrane proteins remains limited owing to challenges in protein expression, purification and the selection of membrane-mimicking systems that will...	nmrlearner	Journal club	0	06-21-2011 01:50 PM
[NMR paper] How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. Related Articles How to prepare membrane proteins for solid-state NMR: A case study on the alpha-helical integral membrane protein diacylglycerol kinase from E. coli. Chembiochem. 2005 Sep;6(9):1693-700 Authors: Lorch M, Faham S, Kaiser C, Weber I, Mason AJ, Bowie JU, Glaubitz C Several studies have demonstrated that it is viable to use microcrystalline preparations of water-soluble proteins as...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] An evaluation of detergents for NMR structural studies of membrane proteins. An evaluation of detergents for NMR structural studies of membrane proteins. Related Articles An evaluation of detergents for NMR structural studies of membrane proteins. J Biomol NMR. 2004 Jan;28(1):43-57 Authors: Krueger-Koplin RD, Sorgen PL, Krueger-Koplin ST, Rivera-Torres IO, Cahill SM, Hicks DB, Grinius L, Krulwich TA, Girvin ME Structural information on membrane proteins lags far behind that on soluble proteins, in large part due to difficulties producing homogeneous, stable, structurally relevant samples in a membrane-like environment....	nmrlearner	Journal club	0	11-24-2010 09:25 PM
[NMR paper] NMR solution structure determination of membrane proteins reconstituted in detergent NMR solution structure determination of membrane proteins reconstituted in detergent micelles. Related Articles NMR solution structure determination of membrane proteins reconstituted in detergent micelles. FEBS Lett. 2003 Nov 27;555(1):144-50 Authors: Fernández C, Wüthrich K As an alternative to X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy in solution can be used for three-dimensional structure determination of small membrane proteins, preferably proteins with beta-barrel fold. This paper reviews recent achievements as...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Solution NMR studies of the integral membrane proteins OmpX and OmpA from Escherichia Solution NMR studies of the integral membrane proteins OmpX and OmpA from Escherichia coli. Related Articles Solution NMR studies of the integral membrane proteins OmpX and OmpA from Escherichia coli. FEBS Lett. 2001 Aug 31;504(3):173-8 Authors: Fernández C, Hilty C, Bonjour S, Adeishvili K, Pervushin K, Wüthrich K Membrane proteins are usually solubilized in polar solvents by incorporation into micelles. Even for small membrane proteins these mixed micelles have rather large molecular masses, typically beyond 50000 Da. The NMR technique TROSY...	nmrlearner	Journal club	0	11-19-2010 08:44 PM
[NMR paper] On choosing a detergent for solution NMR studies of membrane proteins. On choosing a detergent for solution NMR studies of membrane proteins. Related Articles On choosing a detergent for solution NMR studies of membrane proteins. J Biomol NMR. 1998 May;11(4):381-6 Authors: Vinogradova O, Sönnichsen F, Sanders CR Translational diffusion coefficients and catalytic activities were measured for the integral membrane protein diacylglycerol kinase (DAGK) in a variety of types of detergent micelles. Despite the structural diversity of the detergents examined, the translational diffusion coefficients observed for DAGK...	nmrlearner	Journal club	0	11-17-2010 11:06 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off