Related ArticlesSolution NMR Studies of Anesthetic Interactions with Ion Channels.
Methods Enzymol. 2018;603:49-66
Authors: Bondarenko V, Wells M, Xu Y, Tang P
Abstract
NMR spectroscopy is one of the major tools to provide atomic resolution protein structural information. It has been used to elucidate the molecular details of interactions between anesthetics and ion channels, to identify anesthetic binding sites, and to characterize channel dynamics and changes introduced by anesthetics. In this chapter, we present solution NMR methods essential for investigating interactions between ion channels and general anesthetics, including both volatile and intravenous anesthetics. Case studies are provided with a focus on pentameric ligand-gated ion channels and the voltage-gated sodium channel NaChBac.
[NMR paper] A suite of pulse sequences based on multiple sequential acquisitions at one and two radiofrequency channels for solid-state magic-angle spinning NMR studies of proteins.
A suite of pulse sequences based on multiple sequential acquisitions at one and two radiofrequency channels for solid-state magic-angle spinning NMR studies of proteins.
A suite of pulse sequences based on multiple sequential acquisitions at one and two radiofrequency channels for solid-state magic-angle spinning NMR studies of proteins.
J Biomol NMR. 2016 Jun 30;
Authors: Sharma K, Madhu PK, Mote KR
Abstract
One of the fundamental challenges in the application of solid-state NMR is its limited sensitivity, yet a majority of...
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A suite of pulse sequences based on multiple sequential acquisitions at one and two radiofrequency channels for solid-state magic-angle spinning NMR studies of proteins
A suite of pulse sequences based on multiple sequential acquisitions at one and two radiofrequency channels for solid-state magic-angle spinning NMR studies of proteins
Abstract
One of the fundamental challenges in the application of solid-state NMR is its limited sensitivity, yet a majority of experiments do not make efficient use of the limited polarization available. The loss in polarization in a single acquisition experiment is mandated by the need to select out a single coherence pathway. In contrast, sequential acquisition strategies can encode...
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[NMR paper] (19)F-Modified Proteins and (19)F-Containing Ligands as Tools in Solution NMR Studies of Protein Interactions.
(19)F-Modified Proteins and (19)F-Containing Ligands as Tools in Solution NMR Studies of Protein Interactions.
Related Articles (19)F-Modified Proteins and (19)F-Containing Ligands as Tools in Solution NMR Studies of Protein Interactions.
Methods Enzymol. 2015;565:67-95
Authors: Sharaf NG, Gronenborn AM
Abstract
(19)F solution NMR is a powerful and versatile tool to study protein structure and protein-ligand interactions due to the favorable NMR characteristics of the (19)F atom, its absence in naturally occurring...
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[NMR paper] The present and future of solution NMR in investigating the structure and dynamics of channels and transporters.
The present and future of solution NMR in investigating the structure and dynamics of channels and transporters.
The present and future of solution NMR in investigating the structure and dynamics of channels and transporters.
Curr Opin Struct Biol. 2013 Apr 26;
Authors: Oxenoid K, Chou JJ
Abstract
Membrane channels, transporters and receptors constitute essential means for cells to maintain homeostasis and communicate with the surroundings. Investigation of their molecular architecture and the dynamic process of transporting substrate or...
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05-01-2013 11:46 AM
[NMR paper] Insight into the modulation of Shaw2 Kv channels by general anesthetics: structural and functional studies of S4-S5 linker and S6 C-terminal peptides in micelles by NMR.
Insight into the modulation of Shaw2 Kv channels by general anesthetics: structural and functional studies of S4-S5 linker and S6 C-terminal peptides in micelles by NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Insight into the modulation of Shaw2 Kv channels by general anesthetics: structural and functional studies of S4-S5 linker and S6 C-terminal peptides in micelles by NMR.
Biochim Biophys Acta. 2013 Feb;1828(2):595-601
Authors: Zhang J, Qu X, Covarrubias M,...
[NMR paper] Application of the 19F NMR technique to observe binding of the general anesthetic hal
Application of the 19F NMR technique to observe binding of the general anesthetic halothane to human serum albumin.
Related Articles Application of the 19F NMR technique to observe binding of the general anesthetic halothane to human serum albumin.
Anal Sci. 2004 Oct;20(10):1475-7
Authors: Shikii K, Sakurai S, Utsumi H, Seki H, Tashiro M
19F NMR techniques were employed to characterize the binding property of the widely used general anesthetic halothane with human serum albumin (HSA). It was found that 19F(1H) NOE and 2D 1H-19F HOESY...
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[NMR paper] NMR study of volatile anesthetic binding to nicotinic acetylcholine receptors.
NMR study of volatile anesthetic binding to nicotinic acetylcholine receptors.
Related Articles NMR study of volatile anesthetic binding to nicotinic acetylcholine receptors.
Biophys J. 2000 Feb;78(2):746-51
Authors: Xu Y, Seto T, Tang P, Firestone L
New lines of evidence suggest that volatile anesthetics interact specifically with proteins. Direct binding analysis, however, has been largely limited to soluble proteins. In this study, specific interaction was investigated between isoflurane, a clinically important volatile anesthetic, and...
Solution NMR Studies of Anesthetic Interactions with Ion Channels.
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