Solution NMR Structures of the C-Terminal Domain of Tetrahymena Cytoskeletal Protein TCB2 Reveal Distinct Calcium-Induced Structural Rearrangements

		BioNMR > Educational resources > Journal club
Solution NMR Structures of the C-Terminal Domain of Tetrahymena Cytoskeletal Protein TCB2 Reveal Distinct Calcium-Induced Structural Rearrangements

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

02-03-2017, 09:55 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Solution NMR Structures of the C-Terminal Domain of Tetrahymena Cytoskeletal Protein TCB2 Reveal Distinct Calcium-Induced Structural Rearrangements

Solution NMR Structures of the C-Terminal Domain of Tetrahymena Cytoskeletal Protein TCB2 Reveal Distinct Calcium-Induced Structural Rearrangements

Publication date: 3 February 2017
Source:Biophysical Journal, Volume 112, Issue 3, Supplement 1

Author(s): Adina M. Kilpatrick, Jerry E. Honts, Heidi M. Sleister, C. Andrew Fowler

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Solution NMR Structures of the C-domain of Tetrahymena Cytoskeletal Protein Tcb2 Reveal Distinct Calcium-Induced Structural Rearrangements. Solution NMR Structures of the C-domain of Tetrahymena Cytoskeletal Protein Tcb2 Reveal Distinct Calcium-Induced Structural Rearrangements. Related Articles Solution NMR Structures of the C-domain of Tetrahymena Cytoskeletal Protein Tcb2 Reveal Distinct Calcium-Induced Structural Rearrangements. Proteins. 2016 Aug 2; Authors: Kilpatrick AM, Honts JE, Sleister HM, Fowler CA Abstract Tcb2 is a calcium-binding protein that localizes to the membrane-associated skeleton of the ciliated protozoan Tetrahymena thermophila with...	nmrlearner	Journal club	0	08-05-2016 12:26 PM
NMR Structural Studies of the C-Domain of Tcb2, A Calcium Binding Protein from Tetrahymena Thermophila NMR Structural Studies of the C-Domain of Tcb2, A Calcium Binding Protein from Tetrahymena Thermophila Publication date: 16 February 2016 Source:Biophysical Journal, Volume 110, Issue 3, Supplement 1</br> Author(s): Adina M. Kilpatrick, C. Andrew Fowler, Theodore Gurrola, Jerry E. Honts</br> </br></br> </br></br> More...	nmrlearner	Journal club	0	02-17-2016 07:50 PM
[NMR paper] Structural insights into the calcium-mediated allosteric transition in the C-terminal domain of calmodulin from NMR measurements. Structural insights into the calcium-mediated allosteric transition in the C-terminal domain of calmodulin from NMR measurements. Related Articles Structural insights into the calcium-mediated allosteric transition in the C-terminal domain of calmodulin from NMR measurements. Biochemistry. 2015 Nov 30; Authors: Kukic P, Lundström P, Camilloni C, Evenäs J, Akke M, Vendruscolo M Abstract Calmodulin is a two-domain signalling protein that becomes activated upon binding cooperatively two pairs of calcium ions, leading to large-scale...	nmrlearner	Journal club	0	12-01-2015 11:22 PM
Fluorescence Microscopy and Solution NMR Studies of Cytoskeletal Proteins from Tetrahymena Fluorescence Microscopy and Solution NMR Studies of Cytoskeletal Proteins from Tetrahymena Publication date: 27 January 2015 Source:Biophysical Journal, Volume 108, Issue 2, Supplement 1</br> Author(s): Robert Sterner , Jerry Honts , Adina Kilpatrick</br> </br></br> </br></br> More...	nmrlearner	Journal club	0	01-28-2015 05:28 PM
[NMR paper] Solution NMR structure of CD1104B from pathogenic Clostridium difficile reveals a distinct ?-helical architecture and provides first structural representative of protein domain family PF14203. Solution NMR structure of CD1104B from pathogenic Clostridium difficile reveals a distinct ?-helical architecture and provides first structural representative of protein domain family PF14203. Related Articles Solution NMR structure of CD1104B from pathogenic Clostridium difficile reveals a distinct ?-helical architecture and provides first structural representative of protein domain family PF14203. J Struct Funct Genomics. 2013 Sep 19; Authors: Pulavarti SV, Eletsky A, Lee HW, Acton TB, Xiao R, Everett JK, Prestegard JH, Montelione GT, Szyperski T ...	nmrlearner	Journal club	0	09-21-2013 06:50 PM
[NMR paper] Solution NMR structures provide first structural coverage of the large protein domain family PF08369 and complementary structural coverage of dark operative protochlorophyllide oxidoreductase complexes. Solution NMR structures provide first structural coverage of the large protein domain family PF08369 and complementary structural coverage of dark operative protochlorophyllide oxidoreductase complexes. Solution NMR structures provide first structural coverage of the large protein domain family PF08369 and complementary structural coverage of dark operative protochlorophyllide oxidoreductase complexes. J Struct Funct Genomics. 2013 Aug 21; Authors: Pulavarti SV, He Y, Feldmann EA, Eletsky A, Acton TB, Xiao R, Everett JK, Montelione GT, Kennedy MA, Szyperski...	nmrlearner	Journal club	0	08-23-2013 01:07 AM
Solution NMR and X-ray crystal structures of membrane-associated Lipoprotein-17 domain reveal a novel fold. Solution NMR and X-ray crystal structures of membrane-associated Lipoprotein-17 domain reveal a novel fold. Solution NMR and X-ray crystal structures of membrane-associated Lipoprotein-17 domain reveal a novel fold. J Struct Funct Genomics. 2010 Dec 14; Authors: Mani R, Vorobiev S, Swapna GV, Neely H, Janjua H, Ciccosanti C, Xiao R, Acton TB, Everett JK, Hunt J, Montelione GT The conserved Lipoprotein-17 domain of membrane-associated protein Q9PRA0_UREPA from Ureaplasma parvum was selected for structure determination by the Northeast Structural...	nmrlearner	Journal club	0	12-15-2010 12:03 PM
[NMR paper] Calcium-induced peptide association to form an intact protein domain: 1H NMR structur Calcium-induced peptide association to form an intact protein domain: 1H NMR structural evidence. Related Articles Calcium-induced peptide association to form an intact protein domain: 1H NMR structural evidence. Science. 1990 Jul 20;249(4966):280-3 Authors: Shaw GS, Hodges RS, Sykes BD The 70-residue carboxyl-terminal domain of the muscle contractile protein troponin-C contains two helix-loop-helix calcium (Ca)-binding sites that are related to each other by approximate twofold rotational symmetry. Hydrophobic residues from the helices and a...	nmrlearner	Journal club	0	08-21-2010 11:04 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off